Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2210 and Psyr_2211 overlap by 4 nucleotides Psyr_2211 and Psyr_2212 are separated by 196 nucleotides
Psyr_2210: Psyr_2210 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 2,568,228 to 2,569,187
_2210
Psyr_2211: Psyr_2211 - monooxygenase, NtaA/SnaA/SoxA family, at 2,569,184 to 2,570,530
_2211
Psyr_2212: Psyr_2212 - Arginase/agmatinase/formiminoglutamase, at 2,570,727 to 2,571,617
_2212
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2568.364 kb on - strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.538 kb on - strand at 2568.977 kb on - strand, within Psyr_2210 at 2569.012 kb on + strand, within Psyr_2210 at 2569.013 kb on - strand, within Psyr_2210 at 2569.282 kb on - strand at 2569.282 kb on - strand at 2569.338 kb on + strand, within Psyr_2211 at 2569.788 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.859 kb on - strand, within Psyr_2211 at 2570.232 kb on + strand, within Psyr_2211 at 2570.415 kb on + strand at 2570.560 kb on + strand at 2570.560 kb on + strand at 2570.560 kb on + strand at 2570.560 kb on + strand at 2570.561 kb on - strand at 2570.561 kb on - strand at 2570.607 kb on - strand at 2570.721 kb on + strand at 2570.879 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.313 kb on + strand, within Psyr_2212
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,568,364 - Psyr_2210 0.14 +2.6 2,568,537 + Psyr_2210 0.32 -2.8 2,568,537 + Psyr_2210 0.32 +2.6 2,568,537 + Psyr_2210 0.32 -0.9 2,568,538 - -2.3 2,568,977 - Psyr_2210 0.78 -1.1 2,569,012 + Psyr_2210 0.82 +4.1 2,569,013 - Psyr_2210 0.82 +3.3 2,569,282 - +2.4 2,569,282 - +1.4 2,569,338 + Psyr_2211 0.11 -1.2 2,569,788 + Psyr_2211 0.45 +1.1 2,569,791 + Psyr_2211 0.45 +2.6 2,569,791 + Psyr_2211 0.45 -3.4 2,569,791 + Psyr_2211 0.45 -3.7 2,569,859 - Psyr_2211 0.50 -1.3 2,570,232 + Psyr_2211 0.78 +2.4 2,570,415 + +3.5 2,570,560 + +3.9 2,570,560 + +2.7 2,570,560 + -1.1 2,570,560 + -1.4 2,570,561 - +2.1 2,570,561 - +1.4 2,570,607 - +2.1 2,570,721 + -1.2 2,570,879 + Psyr_2212 0.17 +2.4 2,571,133 + Psyr_2212 0.46 -1.1 2,571,133 + Psyr_2212 0.46 -1.8 2,571,133 + Psyr_2212 0.46 -0.1 2,571,133 + Psyr_2212 0.46 +1.4 2,571,133 + Psyr_2212 0.46 -2.1 2,571,133 + Psyr_2212 0.46 -0.9 2,571,134 - Psyr_2212 0.46 +0.2 2,571,134 - Psyr_2212 0.46 +4.1 2,571,134 - Psyr_2212 0.46 +4.2 2,571,134 - Psyr_2212 0.46 -0.4 2,571,134 - Psyr_2212 0.46 -4.3 2,571,134 - Psyr_2212 0.46 +2.9 2,571,134 - Psyr_2212 0.46 +3.7 2,571,134 - Psyr_2212 0.46 +4.2 2,571,134 - Psyr_2212 0.46 +1.9 2,571,134 - Psyr_2212 0.46 +0.4 2,571,313 + Psyr_2212 0.66 +2.7
Or see this region's nucleotide sequence