Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2209 and Psyr_2210 are separated by 76 nucleotides Psyr_2210 and Psyr_2211 overlap by 4 nucleotides
Psyr_2209: Psyr_2209 - ABC transporter, substrate-binding protein, aliphatic sulfonate, at 2,567,177 to 2,568,151
_2209
Psyr_2210: Psyr_2210 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 2,568,228 to 2,569,187
_2210
Psyr_2211: Psyr_2211 - monooxygenase, NtaA/SnaA/SoxA family, at 2,569,184 to 2,570,530
_2211
Position (kb)
2568
2569
2570 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2567.302 kb on - strand, within Psyr_2209 at 2567.481 kb on + strand, within Psyr_2209 at 2567.482 kb on - strand, within Psyr_2209 at 2567.494 kb on - strand, within Psyr_2209 at 2567.691 kb on + strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.818 kb on - strand, within Psyr_2209 at 2568.364 kb on - strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.538 kb on - strand at 2568.977 kb on - strand, within Psyr_2210 at 2569.012 kb on + strand, within Psyr_2210 at 2569.013 kb on - strand, within Psyr_2210 at 2569.282 kb on - strand at 2569.282 kb on - strand at 2569.338 kb on + strand, within Psyr_2211 at 2569.788 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.791 kb on + strand, within Psyr_2211 at 2569.859 kb on - strand, within Psyr_2211
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,567,302 - Psyr_2209 0.13 +1.8 2,567,481 + Psyr_2209 0.31 +3.6 2,567,482 - Psyr_2209 0.31 +3.8 2,567,494 - Psyr_2209 0.33 -1.5 2,567,691 + Psyr_2209 0.53 -0.2 2,567,745 - Psyr_2209 0.58 -3.6 2,567,745 - Psyr_2209 0.58 -3.4 2,567,818 - Psyr_2209 0.66 -1.3 2,568,364 - Psyr_2210 0.14 +2.6 2,568,537 + Psyr_2210 0.32 -2.8 2,568,537 + Psyr_2210 0.32 +2.6 2,568,537 + Psyr_2210 0.32 -0.9 2,568,538 - -2.3 2,568,977 - Psyr_2210 0.78 -1.1 2,569,012 + Psyr_2210 0.82 +4.1 2,569,013 - Psyr_2210 0.82 +3.3 2,569,282 - +2.4 2,569,282 - +1.4 2,569,338 + Psyr_2211 0.11 -1.2 2,569,788 + Psyr_2211 0.45 +1.1 2,569,791 + Psyr_2211 0.45 +2.6 2,569,791 + Psyr_2211 0.45 -3.4 2,569,791 + Psyr_2211 0.45 -3.7 2,569,859 - Psyr_2211 0.50 -1.3
Or see this region's nucleotide sequence