Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2208 and Psyr_2209 are separated by 60 nucleotides Psyr_2209 and Psyr_2210 are separated by 76 nucleotides
Psyr_2208: Psyr_2208 - Amidase, at 2,565,581 to 2,567,116
_2208
Psyr_2209: Psyr_2209 - ABC transporter, substrate-binding protein, aliphatic sulfonate, at 2,567,177 to 2,568,151
_2209
Psyr_2210: Psyr_2210 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 2,568,228 to 2,569,187
_2210
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2566.210 kb on + strand, within Psyr_2208 at 2566.214 kb on + strand, within Psyr_2208 at 2566.214 kb on + strand, within Psyr_2208 at 2566.229 kb on + strand, within Psyr_2208 at 2566.230 kb on - strand, within Psyr_2208 at 2566.643 kb on + strand, within Psyr_2208 at 2566.643 kb on + strand, within Psyr_2208 at 2566.644 kb on - strand, within Psyr_2208 at 2566.664 kb on + strand, within Psyr_2208 at 2566.665 kb on - strand, within Psyr_2208 at 2566.739 kb on + strand, within Psyr_2208 at 2566.767 kb on - strand, within Psyr_2208 at 2566.811 kb on + strand, within Psyr_2208 at 2566.846 kb on - strand, within Psyr_2208 at 2566.907 kb on + strand, within Psyr_2208 at 2566.907 kb on + strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2567.073 kb on - strand at 2567.302 kb on - strand, within Psyr_2209 at 2567.481 kb on + strand, within Psyr_2209 at 2567.482 kb on - strand, within Psyr_2209 at 2567.494 kb on - strand, within Psyr_2209 at 2567.691 kb on + strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.818 kb on - strand, within Psyr_2209 at 2568.364 kb on - strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.538 kb on - strand at 2568.977 kb on - strand, within Psyr_2210 at 2569.012 kb on + strand, within Psyr_2210 at 2569.013 kb on - strand, within Psyr_2210
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,566,210 + Psyr_2208 0.41 -0.9 2,566,214 + Psyr_2208 0.41 +2.4 2,566,214 + Psyr_2208 0.41 -1.0 2,566,229 + Psyr_2208 0.42 -0.1 2,566,230 - Psyr_2208 0.42 +0.2 2,566,643 + Psyr_2208 0.69 +3.4 2,566,643 + Psyr_2208 0.69 +0.9 2,566,644 - Psyr_2208 0.69 +2.9 2,566,664 + Psyr_2208 0.71 +0.6 2,566,665 - Psyr_2208 0.71 +2.7 2,566,739 + Psyr_2208 0.75 -2.0 2,566,767 - Psyr_2208 0.77 +1.9 2,566,811 + Psyr_2208 0.80 -3.2 2,566,846 - Psyr_2208 0.82 +3.6 2,566,907 + Psyr_2208 0.86 -2.7 2,566,907 + Psyr_2208 0.86 +1.4 2,566,908 - Psyr_2208 0.86 +4.0 2,566,908 - Psyr_2208 0.86 +2.4 2,566,908 - Psyr_2208 0.86 +1.4 2,567,073 - +3.0 2,567,302 - Psyr_2209 0.13 +1.8 2,567,481 + Psyr_2209 0.31 +3.6 2,567,482 - Psyr_2209 0.31 +3.8 2,567,494 - Psyr_2209 0.33 -1.5 2,567,691 + Psyr_2209 0.53 -0.2 2,567,745 - Psyr_2209 0.58 -3.6 2,567,745 - Psyr_2209 0.58 -3.4 2,567,818 - Psyr_2209 0.66 -1.3 2,568,364 - Psyr_2210 0.14 +2.6 2,568,537 + Psyr_2210 0.32 -2.8 2,568,537 + Psyr_2210 0.32 +2.6 2,568,537 + Psyr_2210 0.32 -0.9 2,568,538 - -2.3 2,568,977 - Psyr_2210 0.78 -1.1 2,569,012 + Psyr_2210 0.82 +4.1 2,569,013 - Psyr_2210 0.82 +3.3
Or see this region's nucleotide sequence