Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2203 and Psyr_2204 are separated by 1 nucleotides Psyr_2204 and Psyr_2205 are separated by 66 nucleotides Psyr_2205 and Psyr_2206 are separated by 728 nucleotides
Psyr_2203: Psyr_2203 - amino acid/amide ABC transporter membrane protein 2, HAAT family, at 2,559,557 to 2,560,699
_2203
Psyr_2204: Psyr_2204 - amino acid/amide ABC transporter membrane protein 1, HAAT family, at 2,560,701 to 2,561,603
_2204
Psyr_2205: Psyr_2205 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 2,561,670 to 2,562,851
_2205
Psyr_2206: Psyr_2206 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 2,563,580 to 2,564,728
_2206
Position (kb)
2561
2562
2563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2560.805 kb on - strand, within Psyr_2204 at 2561.311 kb on + strand, within Psyr_2204 at 2561.311 kb on + strand, within Psyr_2204 at 2561.312 kb on - strand, within Psyr_2204 at 2561.343 kb on + strand, within Psyr_2204 at 2561.395 kb on + strand, within Psyr_2204 at 2561.396 kb on - strand, within Psyr_2204 at 2561.446 kb on + strand, within Psyr_2204 at 2561.446 kb on + strand, within Psyr_2204 at 2561.446 kb on + strand, within Psyr_2204 at 2561.446 kb on + strand, within Psyr_2204 at 2561.447 kb on - strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.455 kb on + strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.456 kb on - strand, within Psyr_2204 at 2561.584 kb on - strand at 2561.713 kb on + strand at 2561.713 kb on + strand at 2561.896 kb on + strand, within Psyr_2205 at 2561.896 kb on + strand, within Psyr_2205 at 2561.896 kb on + strand, within Psyr_2205 at 2561.897 kb on - strand, within Psyr_2205 at 2561.897 kb on - strand, within Psyr_2205 at 2561.908 kb on + strand, within Psyr_2205 at 2561.908 kb on + strand, within Psyr_2205 at 2561.909 kb on - strand, within Psyr_2205 at 2561.909 kb on - strand, within Psyr_2205 at 2561.909 kb on - strand, within Psyr_2205 at 2562.011 kb on - strand, within Psyr_2205 at 2562.011 kb on - strand, within Psyr_2205 at 2562.011 kb on - strand, within Psyr_2205 at 2562.136 kb on + strand, within Psyr_2205 at 2562.136 kb on + strand, within Psyr_2205 at 2562.144 kb on + strand, within Psyr_2205 at 2562.244 kb on + strand, within Psyr_2205 at 2562.322 kb on + strand, within Psyr_2205 at 2562.323 kb on - strand, within Psyr_2205 at 2562.340 kb on + strand, within Psyr_2205 at 2562.340 kb on + strand, within Psyr_2205 at 2562.420 kb on - strand, within Psyr_2205 at 2562.448 kb on + strand, within Psyr_2205 at 2562.449 kb on - strand, within Psyr_2205 at 2562.449 kb on - strand, within Psyr_2205 at 2562.449 kb on - strand, within Psyr_2205 at 2562.449 kb on - strand, within Psyr_2205 at 2562.463 kb on + strand, within Psyr_2205 at 2562.464 kb on - strand, within Psyr_2205 at 2562.857 kb on + strand at 2562.858 kb on - strand at 2563.034 kb on - strand at 2563.109 kb on - strand at 2563.158 kb on + strand at 2563.158 kb on + strand at 2563.158 kb on + strand at 2563.159 kb on - strand at 2563.201 kb on + strand at 2563.205 kb on + strand at 2563.205 kb on + strand at 2563.205 kb on + strand at 2563.206 kb on - strand at 2563.206 kb on - strand at 2563.215 kb on + strand at 2563.215 kb on + strand at 2563.216 kb on - strand at 2563.216 kb on - strand at 2563.216 kb on - strand at 2563.216 kb on - strand at 2563.225 kb on + strand at 2563.226 kb on - strand at 2563.575 kb on + strand at 2563.575 kb on + strand at 2563.576 kb on - strand at 2563.576 kb on - strand at 2563.602 kb on + strand at 2563.603 kb on - strand at 2563.603 kb on - strand at 2563.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,560,805 - Psyr_2204 0.12 +2.1 2,561,311 + Psyr_2204 0.68 +3.5 2,561,311 + Psyr_2204 0.68 -0.2 2,561,312 - Psyr_2204 0.68 +1.4 2,561,343 + Psyr_2204 0.71 -1.9 2,561,395 + Psyr_2204 0.77 +2.6 2,561,396 - Psyr_2204 0.77 +1.4 2,561,446 + Psyr_2204 0.83 +2.6 2,561,446 + Psyr_2204 0.83 +0.2 2,561,446 + Psyr_2204 0.83 +1.4 2,561,446 + Psyr_2204 0.83 +0.8 2,561,447 - Psyr_2204 0.83 -0.2 2,561,455 + Psyr_2204 0.83 +3.1 2,561,455 + Psyr_2204 0.83 -2.0 2,561,455 + Psyr_2204 0.83 -1.2 2,561,455 + Psyr_2204 0.83 +0.3 2,561,455 + Psyr_2204 0.83 -2.3 2,561,455 + Psyr_2204 0.83 -0.3 2,561,456 - Psyr_2204 0.84 -3.8 2,561,456 - Psyr_2204 0.84 +2.6 2,561,456 - Psyr_2204 0.84 +1.8 2,561,456 - Psyr_2204 0.84 +3.0 2,561,456 - Psyr_2204 0.84 +2.1 2,561,456 - Psyr_2204 0.84 +1.8 2,561,456 - Psyr_2204 0.84 -2.2 2,561,584 - +3.3 2,561,713 + +1.8 2,561,713 + +1.8 2,561,896 + Psyr_2205 0.19 +3.0 2,561,896 + Psyr_2205 0.19 +2.6 2,561,896 + Psyr_2205 0.19 +3.0 2,561,897 - Psyr_2205 0.19 +1.4 2,561,897 - Psyr_2205 0.19 +2.4 2,561,908 + Psyr_2205 0.20 -1.1 2,561,908 + Psyr_2205 0.20 +1.4 2,561,909 - Psyr_2205 0.20 +4.1 2,561,909 - Psyr_2205 0.20 +3.1 2,561,909 - Psyr_2205 0.20 +1.8 2,562,011 - Psyr_2205 0.29 -0.9 2,562,011 - Psyr_2205 0.29 -1.9 2,562,011 - Psyr_2205 0.29 +0.8 2,562,136 + Psyr_2205 0.39 +1.8 2,562,136 + Psyr_2205 0.39 -0.6 2,562,144 + Psyr_2205 0.40 -0.1 2,562,244 + Psyr_2205 0.49 +3.1 2,562,322 + Psyr_2205 0.55 -1.8 2,562,323 - Psyr_2205 0.55 +3.0 2,562,340 + Psyr_2205 0.57 +2.4 2,562,340 + Psyr_2205 0.57 +3.1 2,562,420 - Psyr_2205 0.63 -2.0 2,562,448 + Psyr_2205 0.66 -0.6 2,562,449 - Psyr_2205 0.66 +4.0 2,562,449 - Psyr_2205 0.66 -0.8 2,562,449 - Psyr_2205 0.66 +2.4 2,562,449 - Psyr_2205 0.66 +2.1 2,562,463 + Psyr_2205 0.67 +4.3 2,562,464 - Psyr_2205 0.67 +2.4 2,562,857 + +4.5 2,562,858 - +2.0 2,563,034 - +1.1 2,563,109 - -1.5 2,563,158 + +1.4 2,563,158 + +1.4 2,563,158 + -0.4 2,563,159 - +0.0 2,563,201 + -0.7 2,563,205 + +0.1 2,563,205 + +1.8 2,563,205 + -1.2 2,563,206 - +1.8 2,563,206 - +2.6 2,563,215 + +3.1 2,563,215 + +2.6 2,563,216 - -2.1 2,563,216 - -2.8 2,563,216 - -2.5 2,563,216 - +4.0 2,563,225 + -0.2 2,563,226 - +3.4 2,563,575 + +2.6 2,563,575 + +2.6 2,563,576 - +0.8 2,563,576 - -1.5 2,563,602 + -3.6 2,563,603 - +0.2 2,563,603 - +2.4 2,563,622 - +0.2
Or see this region's nucleotide sequence