Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2195 and Psyr_2196 overlap by 4 nucleotides Psyr_2196 and Psyr_2197 are separated by 27 nucleotides
Psyr_2195: Psyr_2195 - Urease accessory protein UreD, at 2,552,951 to 2,553,808
_2195
Psyr_2196: Psyr_2196 - Urease accessory protein UreG, at 2,553,805 to 2,554,467
_2196
Psyr_2197: Psyr_2197 - urease, Metallo peptidase, MEROPS family M38, at 2,554,495 to 2,556,207
_2197
Position (kb)
2553
2554
2555 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2552.836 kb on - strand at 2552.838 kb on + strand at 2552.838 kb on + strand at 2552.907 kb on + strand at 2553.103 kb on - strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.398 kb on + strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.637 kb on - strand, within Psyr_2195 at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.757 kb on + strand at 2553.757 kb on + strand at 2553.757 kb on + strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.953 kb on + strand, within Psyr_2196 at 2553.953 kb on + strand, within Psyr_2196 at 2553.954 kb on - strand, within Psyr_2196 at 2553.954 kb on - strand, within Psyr_2196 at 2553.972 kb on + strand, within Psyr_2196 at 2554.508 kb on + strand at 2554.509 kb on - strand at 2554.568 kb on + strand at 2554.802 kb on + strand, within Psyr_2197 at 2554.803 kb on - strand, within Psyr_2197 at 2554.940 kb on + strand, within Psyr_2197 at 2554.940 kb on + strand, within Psyr_2197 at 2554.940 kb on + strand, within Psyr_2197 at 2554.941 kb on - strand, within Psyr_2197 at 2554.941 kb on - strand, within Psyr_2197 at 2554.941 kb on - strand, within Psyr_2197 at 2554.941 kb on - strand, within Psyr_2197 at 2554.948 kb on + strand, within Psyr_2197 at 2554.949 kb on - strand, within Psyr_2197 at 2554.979 kb on + strand, within Psyr_2197 at 2554.979 kb on + strand, within Psyr_2197 at 2554.991 kb on + strand, within Psyr_2197 at 2554.991 kb on + strand, within Psyr_2197 at 2555.280 kb on + strand, within Psyr_2197 at 2555.281 kb on - strand, within Psyr_2197 at 2555.281 kb on - strand, within Psyr_2197 at 2555.291 kb on + strand, within Psyr_2197 at 2555.292 kb on - strand, within Psyr_2197 at 2555.390 kb on + strand, within Psyr_2197 at 2555.390 kb on + strand, within Psyr_2197 at 2555.390 kb on + strand, within Psyr_2197 at 2555.391 kb on - strand, within Psyr_2197 at 2555.391 kb on - strand, within Psyr_2197
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,552,836 - +2.4 2,552,838 + +1.8 2,552,838 + +3.4 2,552,907 + +0.4 2,553,103 - Psyr_2195 0.18 -0.8 2,553,346 + Psyr_2195 0.46 -2.9 2,553,346 + Psyr_2195 0.46 -4.5 2,553,346 + Psyr_2195 0.46 +3.6 2,553,347 - Psyr_2195 0.46 -1.4 2,553,347 - Psyr_2195 0.46 -4.0 2,553,347 - Psyr_2195 0.46 -1.4 2,553,347 - Psyr_2195 0.46 -1.2 2,553,347 - Psyr_2195 0.46 +0.4 2,553,347 - Psyr_2195 0.46 -0.9 2,553,398 + Psyr_2195 0.52 -4.6 2,553,399 - Psyr_2195 0.52 -2.4 2,553,399 - Psyr_2195 0.52 -0.7 2,553,457 - Psyr_2195 0.59 +0.0 2,553,457 - Psyr_2195 0.59 +2.8 2,553,637 - Psyr_2195 0.80 -0.2 2,553,723 + +1.4 2,553,723 + +3.1 2,553,723 + -4.7 2,553,724 - -0.1 2,553,724 - +1.8 2,553,724 - -4.0 2,553,724 - +0.8 2,553,724 - -1.9 2,553,757 + -1.1 2,553,757 + -3.1 2,553,757 + -1.0 2,553,758 - -1.8 2,553,758 - +3.3 2,553,758 - -2.2 2,553,758 - -2.3 2,553,758 - +3.3 2,553,953 + Psyr_2196 0.22 -3.6 2,553,953 + Psyr_2196 0.22 -2.7 2,553,954 - Psyr_2196 0.22 -1.9 2,553,954 - Psyr_2196 0.22 +0.1 2,553,972 + Psyr_2196 0.25 -1.1 2,554,508 + +1.4 2,554,509 - -0.1 2,554,568 + +3.3 2,554,802 + Psyr_2197 0.18 +3.5 2,554,803 - Psyr_2197 0.18 +2.6 2,554,940 + Psyr_2197 0.26 -3.3 2,554,940 + Psyr_2197 0.26 +2.1 2,554,940 + Psyr_2197 0.26 +1.8 2,554,941 - Psyr_2197 0.26 +3.0 2,554,941 - Psyr_2197 0.26 +2.1 2,554,941 - Psyr_2197 0.26 +2.6 2,554,941 - Psyr_2197 0.26 +2.6 2,554,948 + Psyr_2197 0.26 -3.3 2,554,949 - Psyr_2197 0.27 -0.6 2,554,979 + Psyr_2197 0.28 -0.4 2,554,979 + Psyr_2197 0.28 +4.2 2,554,991 + Psyr_2197 0.29 -0.2 2,554,991 + Psyr_2197 0.29 +0.8 2,555,280 + Psyr_2197 0.46 +0.5 2,555,281 - Psyr_2197 0.46 +1.8 2,555,281 - Psyr_2197 0.46 -2.6 2,555,291 + Psyr_2197 0.46 +2.4 2,555,292 - Psyr_2197 0.47 +1.4 2,555,390 + Psyr_2197 0.52 +2.1 2,555,390 + Psyr_2197 0.52 +1.0 2,555,390 + Psyr_2197 0.52 -1.8 2,555,391 - Psyr_2197 0.52 +0.2 2,555,391 - Psyr_2197 0.52 +1.8
Or see this region's nucleotide sequence