Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2194 and Psyr_2195 are separated by 225 nucleotides Psyr_2195 and Psyr_2196 overlap by 4 nucleotides Psyr_2196 and Psyr_2197 are separated by 27 nucleotides
Psyr_2194: Psyr_2194 - Acriflavin resistance protein, at 2,549,606 to 2,552,725
_2194
Psyr_2195: Psyr_2195 - Urease accessory protein UreD, at 2,552,951 to 2,553,808
_2195
Psyr_2196: Psyr_2196 - Urease accessory protein UreG, at 2,553,805 to 2,554,467
_2196
Psyr_2197: Psyr_2197 - urease, Metallo peptidase, MEROPS family M38, at 2,554,495 to 2,556,207
_2197
Position (kb)
2552
2553
2554 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2552.160 kb on + strand, within Psyr_2194 at 2552.311 kb on - strand, within Psyr_2194 at 2552.395 kb on + strand, within Psyr_2194 at 2552.395 kb on + strand, within Psyr_2194 at 2552.396 kb on - strand, within Psyr_2194 at 2552.475 kb on + strand at 2552.630 kb on + strand at 2552.630 kb on + strand at 2552.630 kb on + strand at 2552.631 kb on - strand at 2552.631 kb on - strand at 2552.631 kb on - strand at 2552.836 kb on - strand at 2552.838 kb on + strand at 2552.838 kb on + strand at 2552.907 kb on + strand at 2553.103 kb on - strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.346 kb on + strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.347 kb on - strand, within Psyr_2195 at 2553.398 kb on + strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.399 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.457 kb on - strand, within Psyr_2195 at 2553.637 kb on - strand, within Psyr_2195 at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.723 kb on + strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.724 kb on - strand at 2553.757 kb on + strand at 2553.757 kb on + strand at 2553.757 kb on + strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.758 kb on - strand at 2553.953 kb on + strand, within Psyr_2196 at 2553.953 kb on + strand, within Psyr_2196 at 2553.954 kb on - strand, within Psyr_2196 at 2553.954 kb on - strand, within Psyr_2196 at 2553.972 kb on + strand, within Psyr_2196 at 2554.508 kb on + strand at 2554.509 kb on - strand at 2554.568 kb on + strand at 2554.802 kb on + strand, within Psyr_2197 at 2554.803 kb on - strand, within Psyr_2197
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,552,160 + Psyr_2194 0.82 +2.1 2,552,311 - Psyr_2194 0.87 +1.8 2,552,395 + Psyr_2194 0.89 +0.8 2,552,395 + Psyr_2194 0.89 -1.2 2,552,396 - Psyr_2194 0.89 -0.7 2,552,475 + -0.0 2,552,630 + -2.4 2,552,630 + +3.4 2,552,630 + -0.9 2,552,631 - +0.5 2,552,631 - -2.8 2,552,631 - +2.0 2,552,836 - +2.4 2,552,838 + +1.8 2,552,838 + +3.4 2,552,907 + +0.4 2,553,103 - Psyr_2195 0.18 -0.8 2,553,346 + Psyr_2195 0.46 -2.9 2,553,346 + Psyr_2195 0.46 -4.5 2,553,346 + Psyr_2195 0.46 +3.6 2,553,347 - Psyr_2195 0.46 -1.4 2,553,347 - Psyr_2195 0.46 -4.0 2,553,347 - Psyr_2195 0.46 -1.4 2,553,347 - Psyr_2195 0.46 -1.2 2,553,347 - Psyr_2195 0.46 +0.4 2,553,347 - Psyr_2195 0.46 -0.9 2,553,398 + Psyr_2195 0.52 -4.6 2,553,399 - Psyr_2195 0.52 -2.4 2,553,399 - Psyr_2195 0.52 -0.7 2,553,457 - Psyr_2195 0.59 +0.0 2,553,457 - Psyr_2195 0.59 +2.8 2,553,637 - Psyr_2195 0.80 -0.2 2,553,723 + +1.4 2,553,723 + +3.1 2,553,723 + -4.7 2,553,724 - -0.1 2,553,724 - +1.8 2,553,724 - -4.0 2,553,724 - +0.8 2,553,724 - -1.9 2,553,757 + -1.1 2,553,757 + -3.1 2,553,757 + -1.0 2,553,758 - -1.8 2,553,758 - +3.3 2,553,758 - -2.2 2,553,758 - -2.3 2,553,758 - +3.3 2,553,953 + Psyr_2196 0.22 -3.6 2,553,953 + Psyr_2196 0.22 -2.7 2,553,954 - Psyr_2196 0.22 -1.9 2,553,954 - Psyr_2196 0.22 +0.1 2,553,972 + Psyr_2196 0.25 -1.1 2,554,508 + +1.4 2,554,509 - -0.1 2,554,568 + +3.3 2,554,802 + Psyr_2197 0.18 +3.5 2,554,803 - Psyr_2197 0.18 +2.6
Or see this region's nucleotide sequence