Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2181 and Psyr_2182 are separated by 619 nucleotides Psyr_2182 and Psyr_2183 are separated by 177 nucleotides Psyr_2183 and Psyr_2184 overlap by 4 nucleotides
Psyr_2181: Psyr_2181 - Major facilitator superfamily, at 2,535,193 to 2,536,302
_2181
Psyr_2182: Psyr_2182 - hypothetical protein, at 2,536,922 to 2,537,275
_2182
Psyr_2183: Psyr_2183 - transcriptional regulator, GntR family, at 2,537,453 to 2,538,175
_2183
Psyr_2184: Psyr_2184 - Short-chain dehydrogenase/reductase SDR, at 2,538,172 to 2,538,960
_2184
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2535.935 kb on + strand, within Psyr_2181 at 2535.935 kb on + strand, within Psyr_2181 at 2535.936 kb on - strand, within Psyr_2181 at 2536.057 kb on + strand, within Psyr_2181 at 2536.058 kb on - strand, within Psyr_2181 at 2536.058 kb on - strand, within Psyr_2181 at 2536.129 kb on + strand, within Psyr_2181 at 2536.129 kb on + strand, within Psyr_2181 at 2536.129 kb on + strand, within Psyr_2181 at 2536.130 kb on - strand, within Psyr_2181 at 2536.130 kb on - strand, within Psyr_2181 at 2536.169 kb on + strand, within Psyr_2181 at 2536.169 kb on + strand, within Psyr_2181 at 2536.464 kb on + strand at 2536.465 kb on - strand at 2536.544 kb on - strand at 2536.601 kb on + strand at 2536.751 kb on + strand at 2536.752 kb on - strand at 2536.777 kb on + strand at 2536.778 kb on - strand at 2536.788 kb on + strand at 2536.808 kb on + strand at 2536.808 kb on + strand at 2536.809 kb on - strand at 2536.809 kb on - strand at 2536.947 kb on + strand at 2536.950 kb on + strand at 2536.975 kb on - strand, within Psyr_2182 at 2537.053 kb on + strand, within Psyr_2182 at 2537.054 kb on - strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.063 kb on + strand, within Psyr_2182 at 2537.064 kb on - strand, within Psyr_2182 at 2537.064 kb on - strand, within Psyr_2182 at 2537.128 kb on + strand, within Psyr_2182 at 2537.129 kb on - strand, within Psyr_2182 at 2537.164 kb on - strand, within Psyr_2182 at 2537.164 kb on - strand, within Psyr_2182 at 2537.281 kb on + strand at 2537.281 kb on + strand at 2537.281 kb on + strand at 2537.281 kb on + strand at 2537.281 kb on + strand at 2537.281 kb on + strand at 2537.282 kb on - strand at 2537.282 kb on - strand at 2537.282 kb on - strand at 2537.282 kb on - strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.285 kb on + strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.286 kb on - strand at 2537.397 kb on - strand at 2537.397 kb on - strand at 2537.426 kb on + strand at 2537.864 kb on + strand, within Psyr_2183 at 2537.864 kb on + strand, within Psyr_2183 at 2537.864 kb on + strand, within Psyr_2183 at 2537.865 kb on - strand, within Psyr_2183 at 2537.865 kb on - strand, within Psyr_2183 at 2537.865 kb on - strand, within Psyr_2183 at 2537.865 kb on - strand, within Psyr_2183 at 2537.900 kb on + strand, within Psyr_2183
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,535,935 + Psyr_2181 0.67 -0.7 2,535,935 + Psyr_2181 0.67 -0.7 2,535,936 - Psyr_2181 0.67 +0.0 2,536,057 + Psyr_2181 0.78 +4.0 2,536,058 - Psyr_2181 0.78 -1.5 2,536,058 - Psyr_2181 0.78 -0.8 2,536,129 + Psyr_2181 0.84 +0.1 2,536,129 + Psyr_2181 0.84 -1.6 2,536,129 + Psyr_2181 0.84 -2.8 2,536,130 - Psyr_2181 0.84 -3.8 2,536,130 - Psyr_2181 0.84 -3.4 2,536,169 + Psyr_2181 0.88 -2.1 2,536,169 + Psyr_2181 0.88 +0.0 2,536,464 + +0.9 2,536,465 - -0.2 2,536,544 - -2.8 2,536,601 + +2.7 2,536,751 + -1.1 2,536,752 - -1.0 2,536,777 + -2.1 2,536,778 - +3.2 2,536,788 + +2.5 2,536,808 + +4.0 2,536,808 + -1.7 2,536,809 - +0.3 2,536,809 - -1.6 2,536,947 + +0.2 2,536,950 + +1.5 2,536,975 - Psyr_2182 0.15 -3.1 2,537,053 + Psyr_2182 0.37 -1.5 2,537,054 - Psyr_2182 0.37 -1.1 2,537,063 + Psyr_2182 0.40 +2.7 2,537,063 + Psyr_2182 0.40 +1.5 2,537,063 + Psyr_2182 0.40 -0.1 2,537,063 + Psyr_2182 0.40 -2.7 2,537,063 + Psyr_2182 0.40 -0.1 2,537,063 + Psyr_2182 0.40 +2.5 2,537,063 + Psyr_2182 0.40 -2.5 2,537,063 + Psyr_2182 0.40 -2.9 2,537,063 + Psyr_2182 0.40 +2.5 2,537,063 + Psyr_2182 0.40 +1.5 2,537,063 + Psyr_2182 0.40 -2.3 2,537,063 + Psyr_2182 0.40 -2.7 2,537,063 + Psyr_2182 0.40 +1.9 2,537,063 + Psyr_2182 0.40 +3.0 2,537,064 - Psyr_2182 0.40 +3.6 2,537,064 - Psyr_2182 0.40 +2.7 2,537,128 + Psyr_2182 0.58 -2.6 2,537,129 - Psyr_2182 0.58 -3.6 2,537,164 - Psyr_2182 0.68 -1.5 2,537,164 - Psyr_2182 0.68 -3.2 2,537,281 + -2.5 2,537,281 + -0.1 2,537,281 + -1.1 2,537,281 + +2.7 2,537,281 + +0.9 2,537,281 + -1.4 2,537,282 - -0.1 2,537,282 - -2.5 2,537,282 - +0.9 2,537,282 - -3.7 2,537,285 + -1.1 2,537,285 + +0.1 2,537,285 + -3.8 2,537,285 + -1.2 2,537,285 + +0.9 2,537,285 + -0.1 2,537,285 + -1.6 2,537,285 + -1.1 2,537,286 - -3.9 2,537,286 - -2.7 2,537,286 - +0.9 2,537,286 - -0.1 2,537,286 - -0.6 2,537,286 - +2.5 2,537,286 - -0.1 2,537,397 - -0.3 2,537,397 - -1.5 2,537,426 + +1.9 2,537,864 + Psyr_2183 0.57 -1.3 2,537,864 + Psyr_2183 0.57 +1.5 2,537,864 + Psyr_2183 0.57 +0.8 2,537,865 - Psyr_2183 0.57 -1.9 2,537,865 - Psyr_2183 0.57 +2.9 2,537,865 - Psyr_2183 0.57 -0.1 2,537,865 - Psyr_2183 0.57 -2.4 2,537,900 + Psyr_2183 0.62 +3.9
Or see this region's nucleotide sequence