Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2142 and Psyr_2143 are separated by 259 nucleotides Psyr_2143 and Psyr_2144 are separated by 102 nucleotides Psyr_2144 and Psyr_2145 are separated by 87 nucleotides Psyr_2145 and Psyr_2146 overlap by 4 nucleotides
Psyr_2142: Psyr_2142 - FecR protein, at 2,500,866 to 2,501,894
_2142
Psyr_2143: Psyr_2143 - delta1-piperideine 2-carboxylate reductase, at 2,502,154 to 2,503,185
_2143
Psyr_2144: Psyr_2144 - conserved hypothetical protein, at 2,503,288 to 2,503,485
_2144
Psyr_2145: Psyr_2145 - conserved domain protein, at 2,503,573 to 2,503,830
_2145
Psyr_2146: Psyr_2146 - methionine aminopeptidase, type I, at 2,503,827 to 2,504,618
_2146
Position (kb)
2502
2503
2504 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2501.182 kb on + strand, within Psyr_2142 at 2501.183 kb on - strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.291 kb on - strand, within Psyr_2142 at 2501.291 kb on - strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.437 kb on + strand, within Psyr_2142 at 2501.518 kb on + strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.527 kb on + strand, within Psyr_2142 at 2501.740 kb on + strand, within Psyr_2142 at 2501.740 kb on + strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.908 kb on + strand at 2501.908 kb on + strand at 2501.909 kb on - strand at 2501.909 kb on - strand at 2501.909 kb on - strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.926 kb on + strand at 2501.985 kb on + strand at 2502.044 kb on - strand at 2502.080 kb on + strand at 2502.081 kb on - strand at 2502.130 kb on + strand at 2503.049 kb on + strand, within Psyr_2143 at 2503.049 kb on + strand, within Psyr_2143 at 2503.101 kb on + strand at 2503.101 kb on + strand at 2503.102 kb on - strand at 2503.102 kb on - strand at 2503.132 kb on - strand at 2503.192 kb on + strand at 2503.192 kb on + strand at 2503.193 kb on - strand at 2503.193 kb on - strand at 2503.193 kb on - strand at 2503.229 kb on - strand at 2503.297 kb on - strand at 2503.297 kb on - strand at 2503.297 kb on - strand at 2503.332 kb on + strand, within Psyr_2144 at 2503.332 kb on + strand, within Psyr_2144 at 2503.333 kb on - strand, within Psyr_2144 at 2503.333 kb on - strand, within Psyr_2144 at 2503.459 kb on + strand, within Psyr_2144 at 2503.484 kb on - strand at 2503.543 kb on - strand at 2503.711 kb on + strand, within Psyr_2145 at 2503.923 kb on + strand, within Psyr_2146 at 2503.924 kb on - strand, within Psyr_2146 at 2503.924 kb on - strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2504.028 kb on - strand, within Psyr_2146 at 2504.028 kb on - strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.118 kb on - strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.166 kb on - strand, within Psyr_2146 at 2504.166 kb on - strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.170 kb on - strand, within Psyr_2146
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,501,182 + Psyr_2142 0.31 +3.3 2,501,183 - Psyr_2142 0.31 -1.3 2,501,290 + Psyr_2142 0.41 +2.9 2,501,290 + Psyr_2142 0.41 +1.9 2,501,290 + Psyr_2142 0.41 +1.7 2,501,290 + Psyr_2142 0.41 -1.8 2,501,291 - Psyr_2142 0.41 +3.3 2,501,291 - Psyr_2142 0.41 -4.1 2,501,298 + Psyr_2142 0.42 -1.1 2,501,298 + Psyr_2142 0.42 -1.3 2,501,298 + Psyr_2142 0.42 -2.1 2,501,298 + Psyr_2142 0.42 +1.9 2,501,298 + Psyr_2142 0.42 +2.2 2,501,298 + Psyr_2142 0.42 -1.3 2,501,299 - Psyr_2142 0.42 -1.4 2,501,299 - +1.5 2,501,299 - Psyr_2142 0.42 -1.8 2,501,299 - Psyr_2142 0.42 +1.5 2,501,299 - Psyr_2142 0.42 +1.9 2,501,299 - Psyr_2142 0.42 +0.5 2,501,299 - Psyr_2142 0.42 -2.0 2,501,437 + Psyr_2142 0.55 -0.9 2,501,518 + Psyr_2142 0.63 +3.8 2,501,519 - Psyr_2142 0.63 -0.8 2,501,519 - Psyr_2142 0.63 +1.9 2,501,519 - Psyr_2142 0.63 +0.2 2,501,519 - Psyr_2142 0.63 +0.6 2,501,527 + Psyr_2142 0.64 +1.5 2,501,740 + Psyr_2142 0.85 +2.9 2,501,740 + Psyr_2142 0.85 +0.9 2,501,741 - Psyr_2142 0.85 -0.5 2,501,741 - Psyr_2142 0.85 -0.7 2,501,741 - Psyr_2142 0.85 -1.5 2,501,908 + +2.7 2,501,908 + -3.8 2,501,909 - -2.9 2,501,909 - -5.4 2,501,909 - +4.4 2,501,910 + -0.7 2,501,910 + +3.0 2,501,910 + -1.0 2,501,910 + +1.5 2,501,910 + +3.0 2,501,926 + -0.3 2,501,985 + -1.6 2,502,044 - +3.5 2,502,080 + -1.7 2,502,081 - +3.2 2,502,130 + +3.3 2,503,049 + Psyr_2143 0.87 -0.9 2,503,049 + Psyr_2143 0.87 -1.2 2,503,101 + -1.5 2,503,101 + +3.6 2,503,102 - -0.1 2,503,102 - -0.5 2,503,132 - +0.2 2,503,192 + +2.2 2,503,192 + +0.9 2,503,193 - +3.2 2,503,193 - -0.1 2,503,193 - -1.1 2,503,229 - +0.9 2,503,297 - +3.8 2,503,297 - -1.0 2,503,297 - -1.7 2,503,332 + Psyr_2144 0.22 -1.9 2,503,332 + Psyr_2144 0.22 +1.5 2,503,333 - Psyr_2144 0.23 +1.5 2,503,333 - Psyr_2144 0.23 +4.0 2,503,459 + Psyr_2144 0.86 +2.5 2,503,484 - +2.9 2,503,543 - +3.5 2,503,711 + Psyr_2145 0.53 -1.4 2,503,923 + Psyr_2146 0.12 -3.2 2,503,924 - Psyr_2146 0.12 -4.1 2,503,924 - Psyr_2146 0.12 -0.4 2,503,974 + Psyr_2146 0.19 +1.9 2,503,974 + Psyr_2146 0.19 +2.2 2,503,974 + Psyr_2146 0.19 -2.0 2,503,974 + Psyr_2146 0.19 -0.1 2,503,974 + Psyr_2146 0.19 -0.8 2,503,974 + Psyr_2146 0.19 +1.9 2,503,974 + Psyr_2146 0.19 -0.5 2,503,974 + Psyr_2146 0.19 +1.9 2,503,974 + Psyr_2146 0.19 +0.9 2,503,975 - Psyr_2146 0.19 +1.9 2,503,975 - Psyr_2146 0.19 -2.4 2,503,975 - Psyr_2146 0.19 -1.3 2,503,975 - Psyr_2146 0.19 -1.3 2,503,975 - Psyr_2146 0.19 -0.3 2,503,975 - Psyr_2146 0.19 -1.0 2,503,975 - Psyr_2146 0.19 -1.2 2,504,028 - Psyr_2146 0.25 -0.5 2,504,028 - Psyr_2146 0.25 +2.2 2,504,074 + Psyr_2146 0.31 -2.1 2,504,074 + Psyr_2146 0.31 -2.0 2,504,074 + Psyr_2146 0.31 +4.1 2,504,075 - Psyr_2146 0.31 +1.5 2,504,075 - Psyr_2146 0.31 -1.0 2,504,075 - Psyr_2146 0.31 +1.9 2,504,118 - Psyr_2146 0.37 -2.2 2,504,165 + Psyr_2146 0.43 +1.2 2,504,165 + Psyr_2146 0.43 -1.1 2,504,165 + Psyr_2146 0.43 +0.9 2,504,165 + Psyr_2146 0.43 +3.6 2,504,166 - Psyr_2146 0.43 -1.5 2,504,166 - Psyr_2146 0.43 +2.2 2,504,169 + Psyr_2146 0.43 -0.7 2,504,169 + Psyr_2146 0.43 -0.1 2,504,169 + Psyr_2146 0.43 -0.6 2,504,170 - Psyr_2146 0.43 -1.0
Or see this region's nucleotide sequence