Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2139 and Psyr_2140 are separated by 246 nucleotides Psyr_2140 and Psyr_2141 are separated by 19 nucleotides Psyr_2141 and Psyr_2142 are separated by 225 nucleotides
Psyr_2139: Psyr_2139 - Kef-type potassium/proton antiporter, CPA2 family, at 2,497,985 to 2,499,793
_2139
Psyr_2140: Psyr_2140 - conserved hypothetical protein, at 2,500,040 to 2,500,228
_2140
Psyr_2141: Psyr_2141 - hypothetical protein, at 2,500,248 to 2,500,640
_2141
Psyr_2142: Psyr_2142 - FecR protein, at 2,500,866 to 2,501,894
_2142
Position (kb)
2500
2501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2499.101 kb on + strand, within Psyr_2139 at 2499.506 kb on + strand, within Psyr_2139 at 2499.506 kb on + strand, within Psyr_2139 at 2499.507 kb on - strand, within Psyr_2139 at 2499.507 kb on - strand, within Psyr_2139 at 2499.522 kb on - strand, within Psyr_2139 at 2499.596 kb on + strand, within Psyr_2139 at 2499.617 kb on - strand at 2499.687 kb on + strand at 2499.688 kb on - strand at 2499.755 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.795 kb on + strand at 2499.796 kb on - strand at 2499.796 kb on - strand at 2499.811 kb on + strand at 2499.812 kb on - strand at 2499.841 kb on + strand at 2499.861 kb on - strand at 2499.906 kb on + strand at 2499.906 kb on + strand at 2499.984 kb on + strand at 2499.985 kb on - strand at 2499.985 kb on - strand at 2500.157 kb on + strand, within Psyr_2140 at 2500.157 kb on + strand, within Psyr_2140 at 2500.157 kb on + strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.226 kb on + strand at 2500.227 kb on - strand at 2500.309 kb on + strand, within Psyr_2141 at 2500.309 kb on + strand, within Psyr_2141 at 2500.309 kb on + strand, within Psyr_2141 at 2500.310 kb on - strand, within Psyr_2141 at 2500.310 kb on - strand, within Psyr_2141 at 2500.420 kb on - strand, within Psyr_2141 at 2500.665 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.680 kb on - strand at 2500.680 kb on - strand at 2500.682 kb on + strand at 2500.683 kb on - strand at 2500.683 kb on - strand at 2500.683 kb on - strand at 2500.713 kb on - strand at 2500.778 kb on - strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.990 kb on + strand, within Psyr_2142 at 2500.991 kb on - strand, within Psyr_2142 at 2500.991 kb on - strand, within Psyr_2142 at 2501.182 kb on + strand, within Psyr_2142 at 2501.183 kb on - strand, within Psyr_2142
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,499,101 + Psyr_2139 0.62 +2.5 2,499,506 + Psyr_2139 0.84 +2.5 2,499,506 + Psyr_2139 0.84 -0.1 2,499,507 - Psyr_2139 0.84 -1.1 2,499,507 - Psyr_2139 0.84 +0.9 2,499,522 - Psyr_2139 0.85 -1.3 2,499,596 + Psyr_2139 0.89 -2.4 2,499,617 - -0.5 2,499,687 + -0.6 2,499,688 - -1.8 2,499,755 + -3.0 2,499,795 + -0.9 2,499,795 + +0.9 2,499,795 + +1.5 2,499,795 + +2.7 2,499,795 + +1.5 2,499,795 + -2.4 2,499,795 + -1.3 2,499,796 - -0.1 2,499,796 - -0.6 2,499,811 + -1.2 2,499,812 - -1.1 2,499,841 + -0.5 2,499,861 - +3.0 2,499,906 + +3.3 2,499,906 + -0.1 2,499,984 + +2.9 2,499,985 - -0.1 2,499,985 - +1.5 2,500,157 + Psyr_2140 0.62 +2.2 2,500,157 + Psyr_2140 0.62 +0.1 2,500,157 + Psyr_2140 0.62 +2.5 2,500,158 - Psyr_2140 0.62 +0.0 2,500,158 - Psyr_2140 0.62 +3.5 2,500,158 - Psyr_2140 0.62 +3.2 2,500,158 - Psyr_2140 0.62 -0.1 2,500,158 - Psyr_2140 0.62 +1.0 2,500,226 + +3.3 2,500,227 - +3.5 2,500,309 + Psyr_2141 0.16 +2.5 2,500,309 + Psyr_2141 0.16 +1.5 2,500,309 + Psyr_2141 0.16 -0.4 2,500,310 - Psyr_2141 0.16 -1.7 2,500,310 - Psyr_2141 0.16 -1.5 2,500,420 - Psyr_2141 0.44 +2.2 2,500,665 + -0.4 2,500,679 + +0.9 2,500,679 + +1.5 2,500,679 + -2.2 2,500,679 + +1.9 2,500,680 - -1.5 2,500,680 - +3.5 2,500,682 + +1.9 2,500,683 - -3.6 2,500,683 - -2.3 2,500,683 - -2.9 2,500,713 - +3.5 2,500,778 - -1.8 2,500,933 + -0.1 2,500,933 + -0.8 2,500,933 + +2.5 2,500,933 + -3.7 2,500,933 + -0.1 2,500,933 + +4.1 2,500,934 - -2.5 2,500,934 - -0.1 2,500,934 - +0.9 2,500,934 - +0.9 2,500,934 - -0.7 2,500,934 - -1.3 2,500,934 - +1.5 2,500,934 - +1.9 2,500,934 - +0.9 2,500,934 - +0.9 2,500,990 + Psyr_2142 0.12 -3.0 2,500,991 - Psyr_2142 0.12 -0.1 2,500,991 - Psyr_2142 0.12 +0.9 2,501,182 + Psyr_2142 0.31 +3.3 2,501,183 - Psyr_2142 0.31 -1.3
Or see this region's nucleotide sequence