Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2135 and Psyr_2136 are separated by 75 nucleotides Psyr_2136 and Psyr_2137 are separated by 6 nucleotides Psyr_2137 and Psyr_2138 are separated by 89 nucleotides
Psyr_2135: Psyr_2135 - molybdenum cofactor guanylyltransferase, at 2,494,494 to 2,495,099
_2135
Psyr_2136: Psyr_2136 - Molybdenum cofactor biosynthesis protein, at 2,495,175 to 2,495,714
_2136
Psyr_2137: Psyr_2137 - molybdopterin molybdochelatase, at 2,495,721 to 2,496,932
_2137
Psyr_2138: Psyr_2138 - Glycosyl transferase, family 2, at 2,497,022 to 2,497,948
_2138
Position (kb)
2495
2496
2497 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2494.785 kb on + strand, within Psyr_2135 at 2494.785 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2495.066 kb on + strand at 2495.066 kb on + strand at 2495.067 kb on - strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.137 kb on - strand at 2495.137 kb on - strand at 2495.137 kb on - strand at 2495.138 kb on + strand at 2495.138 kb on + strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.204 kb on - strand at 2495.210 kb on + strand at 2495.210 kb on + strand at 2495.211 kb on - strand at 2495.211 kb on - strand at 2495.341 kb on - strand, within Psyr_2136 at 2496.330 kb on + strand, within Psyr_2137 at 2496.330 kb on + strand, within Psyr_2137 at 2496.330 kb on + strand, within Psyr_2137 at 2496.331 kb on - strand, within Psyr_2137 at 2496.667 kb on - strand, within Psyr_2137 at 2496.823 kb on + strand at 2496.824 kb on - strand at 2496.968 kb on - strand at 2496.968 kb on - strand at 2497.015 kb on + strand at 2497.015 kb on + strand at 2497.015 kb on + strand at 2497.015 kb on + strand at 2497.015 kb on + strand at 2497.015 kb on + strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.016 kb on - strand at 2497.058 kb on + strand at 2497.059 kb on - strand at 2497.059 kb on - strand at 2497.263 kb on - strand, within Psyr_2138 at 2497.263 kb on - strand, within Psyr_2138 at 2497.263 kb on - strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.271 kb on + strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.272 kb on - strand, within Psyr_2138 at 2497.295 kb on + strand, within Psyr_2138 at 2497.295 kb on + strand, within Psyr_2138 at 2497.295 kb on + strand, within Psyr_2138 at 2497.295 kb on + strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.296 kb on - strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.580 kb on + strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.581 kb on - strand, within Psyr_2138 at 2497.622 kb on + strand, within Psyr_2138 at 2497.658 kb on + strand, within Psyr_2138 at 2497.658 kb on + strand, within Psyr_2138 at 2497.658 kb on + strand, within Psyr_2138 at 2497.659 kb on - strand, within Psyr_2138 at 2497.659 kb on - strand at 2497.659 kb on - strand, within Psyr_2138 at 2497.766 kb on + strand, within Psyr_2138 at 2497.766 kb on + strand, within Psyr_2138 at 2497.766 kb on + strand, within Psyr_2138 at 2497.834 kb on + strand, within Psyr_2138 at 2497.856 kb on + strand at 2497.857 kb on - strand at 2497.857 kb on - strand at 2497.916 kb on + strand at 2497.916 kb on + strand at 2497.917 kb on - strand at 2497.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,494,785 + Psyr_2135 0.48 -0.4 2,494,785 + Psyr_2135 0.48 -1.6 2,494,842 + Psyr_2135 0.57 -2.8 2,494,842 + Psyr_2135 0.57 -1.4 2,494,843 - Psyr_2135 0.58 +0.8 2,494,843 - Psyr_2135 0.58 +3.2 2,495,066 + +3.3 2,495,066 + -0.1 2,495,067 - -0.6 2,495,136 + +3.2 2,495,136 + +0.4 2,495,136 + -0.8 2,495,136 + +3.5 2,495,137 - -1.6 2,495,137 - +3.3 2,495,137 - +2.9 2,495,138 + -1.0 2,495,138 + +1.9 2,495,139 - +4.0 2,495,139 - +3.5 2,495,139 - -2.6 2,495,139 - -4.6 2,495,139 - -1.3 2,495,139 - -1.3 2,495,204 - +0.9 2,495,210 + +0.9 2,495,210 + +2.2 2,495,211 - +0.2 2,495,211 - +3.3 2,495,341 - Psyr_2136 0.31 +3.6 2,496,330 + Psyr_2137 0.50 +2.9 2,496,330 + Psyr_2137 0.50 -0.1 2,496,330 + Psyr_2137 0.50 +0.9 2,496,331 - Psyr_2137 0.50 -1.8 2,496,667 - Psyr_2137 0.78 -1.1 2,496,823 + +2.2 2,496,824 - +0.9 2,496,968 - +1.5 2,496,968 - -1.7 2,497,015 + -0.8 2,497,015 + -0.3 2,497,015 + -2.3 2,497,015 + -1.8 2,497,015 + +2.7 2,497,015 + -2.7 2,497,016 - +3.3 2,497,016 - +0.9 2,497,016 - +3.0 2,497,016 - -2.3 2,497,016 - +1.5 2,497,016 - +2.5 2,497,016 - -1.5 2,497,058 + +0.6 2,497,059 - -1.1 2,497,059 - +3.2 2,497,263 - Psyr_2138 0.26 -1.1 2,497,263 - Psyr_2138 0.26 +1.9 2,497,263 - Psyr_2138 0.26 +0.3 2,497,271 + Psyr_2138 0.27 -1.2 2,497,271 + Psyr_2138 0.27 +0.9 2,497,271 + Psyr_2138 0.27 +1.9 2,497,271 + Psyr_2138 0.27 -4.9 2,497,271 + Psyr_2138 0.27 +1.5 2,497,271 + Psyr_2138 0.27 +2.5 2,497,271 + Psyr_2138 0.27 -2.4 2,497,271 + Psyr_2138 0.27 -2.3 2,497,271 + Psyr_2138 0.27 +0.1 2,497,271 + Psyr_2138 0.27 +1.2 2,497,271 + Psyr_2138 0.27 -3.7 2,497,272 - Psyr_2138 0.27 +2.9 2,497,272 - Psyr_2138 0.27 +2.7 2,497,272 - Psyr_2138 0.27 +4.0 2,497,272 - Psyr_2138 0.27 -3.8 2,497,272 - Psyr_2138 0.27 -1.6 2,497,272 - Psyr_2138 0.27 +1.5 2,497,272 - Psyr_2138 0.27 +1.5 2,497,272 - Psyr_2138 0.27 +4.3 2,497,272 - Psyr_2138 0.27 +0.6 2,497,272 - Psyr_2138 0.27 +3.0 2,497,272 - Psyr_2138 0.27 +2.7 2,497,272 - Psyr_2138 0.27 -2.0 2,497,295 + Psyr_2138 0.29 +1.9 2,497,295 + Psyr_2138 0.29 -2.4 2,497,295 + Psyr_2138 0.29 +0.9 2,497,295 + Psyr_2138 0.29 +1.2 2,497,296 - Psyr_2138 0.30 +3.5 2,497,296 - Psyr_2138 0.30 +2.7 2,497,296 - Psyr_2138 0.30 +3.3 2,497,296 - Psyr_2138 0.30 +2.4 2,497,296 - Psyr_2138 0.30 +3.7 2,497,296 - Psyr_2138 0.30 +2.9 2,497,580 + Psyr_2138 0.60 +1.9 2,497,580 + Psyr_2138 0.60 +0.9 2,497,580 + Psyr_2138 0.60 -0.1 2,497,580 + Psyr_2138 0.60 -0.1 2,497,580 + Psyr_2138 0.60 +3.0 2,497,580 + Psyr_2138 0.60 -1.5 2,497,580 + Psyr_2138 0.60 -0.5 2,497,580 + Psyr_2138 0.60 -5.0 2,497,580 + Psyr_2138 0.60 +0.6 2,497,580 + Psyr_2138 0.60 +2.2 2,497,580 + Psyr_2138 0.60 +3.6 2,497,580 + Psyr_2138 0.60 -1.8 2,497,580 + Psyr_2138 0.60 -1.2 2,497,580 + Psyr_2138 0.60 +4.3 2,497,580 + Psyr_2138 0.60 +2.9 2,497,580 + Psyr_2138 0.60 +3.0 2,497,580 + Psyr_2138 0.60 -2.5 2,497,580 + Psyr_2138 0.60 -0.9 2,497,580 + Psyr_2138 0.60 -1.2 2,497,581 - Psyr_2138 0.60 +2.2 2,497,581 - Psyr_2138 0.60 +3.7 2,497,581 - Psyr_2138 0.60 -1.3 2,497,581 - Psyr_2138 0.60 -1.6 2,497,581 - Psyr_2138 0.60 +4.2 2,497,581 - Psyr_2138 0.60 +0.9 2,497,581 - Psyr_2138 0.60 +1.9 2,497,581 - Psyr_2138 0.60 +1.4 2,497,581 - Psyr_2138 0.60 -0.1 2,497,581 - Psyr_2138 0.60 +2.7 2,497,581 - Psyr_2138 0.60 +1.9 2,497,581 - Psyr_2138 0.60 -2.5 2,497,581 - Psyr_2138 0.60 -0.1 2,497,581 - Psyr_2138 0.60 -5.2 2,497,581 - Psyr_2138 0.60 +2.7 2,497,581 - Psyr_2138 0.60 +1.5 2,497,581 - Psyr_2138 0.60 +0.9 2,497,581 - Psyr_2138 0.60 +2.5 2,497,622 + Psyr_2138 0.65 -3.0 2,497,658 + Psyr_2138 0.69 +3.5 2,497,658 + Psyr_2138 0.69 +1.9 2,497,658 + Psyr_2138 0.69 -1.4 2,497,659 - Psyr_2138 0.69 +1.1 2,497,659 - +1.5 2,497,659 - Psyr_2138 0.69 -1.2 2,497,766 + Psyr_2138 0.80 -0.1 2,497,766 + Psyr_2138 0.80 +1.2 2,497,766 + Psyr_2138 0.80 -0.0 2,497,834 + Psyr_2138 0.88 -1.6 2,497,856 + +0.7 2,497,857 - -2.2 2,497,857 - -2.9 2,497,916 + -0.8 2,497,916 + -1.9 2,497,917 - -2.9 2,497,917 - -5.6
Or see this region's nucleotide sequence