Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2106 and Psyr_2107 are separated by 141 nucleotides Psyr_2107 and Psyr_2108 are separated by 587 nucleotides Psyr_2108 and Psyr_2109 are separated by 514 nucleotides
Psyr_2106: Psyr_2106 - dihydroorotate oxidase A, at 2,458,192 to 2,459,223
_2106
Psyr_2107: Psyr_2107 - Ribosome modulation factor, at 2,459,365 to 2,459,580
_2107
Psyr_2108: Psyr_2108 - 23S rRNA m(2)G-2445 methyltransferase, at 2,460,168 to 2,462,420
_2108
Psyr_2109: Psyr_2109 - methyl-accepting chemotaxis sensory transducer, at 2,462,935 to 2,464,554
_2109
Position (kb)
2460
2461
2462
2463 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2459.323 kb on + strand at 2459.323 kb on + strand at 2459.323 kb on + strand at 2459.323 kb on + strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.324 kb on - strand at 2459.418 kb on + strand, within Psyr_2107 at 2459.487 kb on + strand, within Psyr_2107 at 2459.487 kb on + strand, within Psyr_2107 at 2459.487 kb on + strand, within Psyr_2107 at 2459.488 kb on - strand, within Psyr_2107 at 2459.488 kb on - strand, within Psyr_2107 at 2459.488 kb on - strand, within Psyr_2107 at 2459.511 kb on + strand, within Psyr_2107 at 2459.511 kb on + strand, within Psyr_2107 at 2459.511 kb on + strand, within Psyr_2107 at 2459.525 kb on + strand, within Psyr_2107 at 2459.531 kb on + strand, within Psyr_2107 at 2459.568 kb on + strand at 2459.568 kb on + strand at 2459.568 kb on + strand at 2459.569 kb on - strand at 2459.569 kb on - strand at 2459.581 kb on - strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.607 kb on + strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.608 kb on - strand at 2459.610 kb on + strand at 2459.611 kb on - strand at 2459.762 kb on + strand at 2459.882 kb on + strand at 2459.883 kb on - strand at 2459.926 kb on - strand at 2459.979 kb on + strand at 2460.013 kb on - strand at 2460.013 kb on - strand at 2460.013 kb on - strand at 2460.015 kb on + strand at 2460.016 kb on - strand at 2460.163 kb on + strand at 2460.163 kb on + strand at 2460.163 kb on + strand at 2460.164 kb on - strand at 2460.164 kb on - strand at 2460.164 kb on - strand at 2460.164 kb on - strand at 2460.394 kb on - strand, within Psyr_2108 at 2460.743 kb on + strand, within Psyr_2108 at 2460.744 kb on - strand, within Psyr_2108 at 2461.008 kb on - strand, within Psyr_2108 at 2461.048 kb on - strand, within Psyr_2108 at 2461.158 kb on + strand, within Psyr_2108 at 2461.158 kb on + strand, within Psyr_2108 at 2461.158 kb on + strand, within Psyr_2108 at 2461.158 kb on + strand, within Psyr_2108 at 2461.159 kb on - strand, within Psyr_2108 at 2461.159 kb on - strand, within Psyr_2108 at 2461.203 kb on - strand, within Psyr_2108 at 2461.275 kb on + strand, within Psyr_2108 at 2461.578 kb on + strand, within Psyr_2108 at 2461.578 kb on + strand, within Psyr_2108 at 2461.620 kb on + strand, within Psyr_2108 at 2461.620 kb on + strand, within Psyr_2108 at 2461.620 kb on + strand, within Psyr_2108 at 2461.648 kb on - strand, within Psyr_2108 at 2461.913 kb on + strand, within Psyr_2108 at 2461.913 kb on + strand, within Psyr_2108 at 2461.960 kb on - strand, within Psyr_2108 at 2462.034 kb on + strand, within Psyr_2108 at 2462.034 kb on + strand, within Psyr_2108 at 2462.034 kb on + strand, within Psyr_2108 at 2462.034 kb on + strand, within Psyr_2108 at 2462.035 kb on - strand, within Psyr_2108 at 2462.035 kb on - strand, within Psyr_2108 at 2462.272 kb on - strand at 2462.272 kb on - strand at 2462.272 kb on - strand at 2462.272 kb on - strand at 2462.343 kb on - strand at 2462.414 kb on + strand at 2462.564 kb on - strand at 2462.596 kb on - strand at 2462.928 kb on + strand at 2462.929 kb on - strand at 2462.929 kb on - strand at 2462.976 kb on + strand at 2462.976 kb on + strand at 2462.977 kb on - strand at 2463.086 kb on + strand at 2463.086 kb on + strand at 2463.086 kb on + strand at 2463.087 kb on - strand at 2463.094 kb on - strand at 2463.101 kb on + strand, within Psyr_2109 at 2463.101 kb on + strand, within Psyr_2109 at 2463.101 kb on + strand, within Psyr_2109 at 2463.102 kb on - strand, within Psyr_2109 at 2463.102 kb on - strand, within Psyr_2109 at 2463.102 kb on - strand, within Psyr_2109 at 2463.232 kb on + strand, within Psyr_2109 at 2463.382 kb on - strand, within Psyr_2109 at 2463.382 kb on - strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.383 kb on + strand, within Psyr_2109 at 2463.384 kb on - strand, within Psyr_2109 at 2463.384 kb on - strand, within Psyr_2109
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,459,323 + -1.8 2,459,323 + -0.1 2,459,323 + +0.3 2,459,323 + -2.0 2,459,324 - +0.6 2,459,324 - -1.1 2,459,324 - -0.6 2,459,324 - -1.5 2,459,324 - -1.8 2,459,324 - -0.4 2,459,324 - -0.2 2,459,324 - +0.3 2,459,324 - -4.3 2,459,418 + Psyr_2107 0.25 +2.2 2,459,487 + Psyr_2107 0.56 +0.9 2,459,487 + Psyr_2107 0.56 -0.2 2,459,487 + Psyr_2107 0.56 +1.5 2,459,488 - Psyr_2107 0.57 -0.1 2,459,488 - Psyr_2107 0.57 +2.2 2,459,488 - Psyr_2107 0.57 -0.3 2,459,511 + Psyr_2107 0.68 +2.9 2,459,511 + Psyr_2107 0.68 -0.6 2,459,511 + Psyr_2107 0.68 +2.8 2,459,525 + Psyr_2107 0.74 -2.7 2,459,531 + Psyr_2107 0.77 +3.1 2,459,568 + +2.7 2,459,568 + +3.7 2,459,568 + -0.1 2,459,569 - +3.1 2,459,569 - -2.6 2,459,581 - +3.6 2,459,607 + -0.1 2,459,607 + +3.3 2,459,607 + +0.7 2,459,607 + -2.3 2,459,607 + -0.8 2,459,607 + +0.5 2,459,607 + -0.7 2,459,608 - +0.1 2,459,608 - +3.2 2,459,608 - +3.1 2,459,608 - +1.9 2,459,608 - +1.9 2,459,608 - -2.8 2,459,608 - +1.9 2,459,610 + -2.3 2,459,611 - -0.6 2,459,762 + -0.1 2,459,882 + +3.3 2,459,883 - -0.5 2,459,926 - -2.9 2,459,979 + -0.6 2,460,013 - +0.0 2,460,013 - +2.7 2,460,013 - -1.1 2,460,015 + -0.3 2,460,016 - -0.9 2,460,163 + +0.9 2,460,163 + +3.9 2,460,163 + +3.9 2,460,164 - -2.5 2,460,164 - +2.9 2,460,164 - +1.5 2,460,164 - +1.9 2,460,394 - Psyr_2108 0.10 +1.9 2,460,743 + Psyr_2108 0.26 -2.6 2,460,744 - Psyr_2108 0.26 +1.5 2,461,008 - Psyr_2108 0.37 +3.1 2,461,048 - Psyr_2108 0.39 -4.0 2,461,158 + Psyr_2108 0.44 -0.4 2,461,158 + Psyr_2108 0.44 +1.5 2,461,158 + Psyr_2108 0.44 +1.1 2,461,158 + Psyr_2108 0.44 +3.1 2,461,159 - Psyr_2108 0.44 +1.9 2,461,159 - Psyr_2108 0.44 +2.9 2,461,203 - Psyr_2108 0.46 +3.5 2,461,275 + Psyr_2108 0.49 +3.2 2,461,578 + Psyr_2108 0.63 +1.5 2,461,578 + Psyr_2108 0.63 +2.5 2,461,620 + Psyr_2108 0.64 +0.4 2,461,620 + Psyr_2108 0.64 -1.7 2,461,620 + Psyr_2108 0.64 -0.7 2,461,648 - Psyr_2108 0.66 +0.9 2,461,913 + Psyr_2108 0.77 -0.5 2,461,913 + Psyr_2108 0.77 -1.6 2,461,960 - Psyr_2108 0.80 +3.6 2,462,034 + Psyr_2108 0.83 +1.9 2,462,034 + Psyr_2108 0.83 -2.2 2,462,034 + Psyr_2108 0.83 +2.9 2,462,034 + Psyr_2108 0.83 -1.0 2,462,035 - Psyr_2108 0.83 -2.7 2,462,035 - Psyr_2108 0.83 +3.6 2,462,272 - -0.2 2,462,272 - +2.7 2,462,272 - +0.3 2,462,272 - +2.9 2,462,343 - +2.7 2,462,414 + -1.2 2,462,564 - +1.9 2,462,596 - -2.0 2,462,928 + -1.3 2,462,929 - -2.8 2,462,929 - +1.5 2,462,976 + -3.1 2,462,976 + +4.3 2,462,977 - +2.5 2,463,086 + +2.2 2,463,086 + -1.6 2,463,086 + +3.5 2,463,087 - +0.9 2,463,094 - +0.3 2,463,101 + Psyr_2109 0.10 -0.5 2,463,101 + Psyr_2109 0.10 -1.3 2,463,101 + Psyr_2109 0.10 +0.4 2,463,102 - Psyr_2109 0.10 -3.6 2,463,102 - Psyr_2109 0.10 -3.7 2,463,102 - Psyr_2109 0.10 -2.9 2,463,232 + Psyr_2109 0.18 -2.4 2,463,382 - Psyr_2109 0.28 +3.9 2,463,382 - Psyr_2109 0.28 -1.8 2,463,383 + Psyr_2109 0.28 +0.9 2,463,383 + Psyr_2109 0.28 -1.3 2,463,383 + Psyr_2109 0.28 -1.4 2,463,383 + Psyr_2109 0.28 -2.1 2,463,383 + Psyr_2109 0.28 -0.9 2,463,383 + Psyr_2109 0.28 +0.9 2,463,383 + Psyr_2109 0.28 +2.9 2,463,383 + Psyr_2109 0.28 -2.5 2,463,383 + Psyr_2109 0.28 +1.5 2,463,383 + Psyr_2109 0.28 -2.4 2,463,384 - Psyr_2109 0.28 -0.6 2,463,384 - Psyr_2109 0.28 +4.3
Or see this region's nucleotide sequence