Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2093 and Psyr_2094 are separated by 85 nucleotides Psyr_2094 and Psyr_2095 are separated by 2 nucleotides Psyr_2095 and Psyr_2096 are separated by 82 nucleotides Psyr_2096 and Psyr_2097 are separated by 105 nucleotides
Psyr_2093: Psyr_2093 - Mg2+ transporter protein, CorA-like protein, at 2,441,780 to 2,442,775
_2093
Psyr_2094: Psyr_2094 - crfX protein, at 2,442,861 to 2,443,109
_2094
Psyr_2095: Psyr_2095 - Conserved TM helix, at 2,443,112 to 2,443,936
_2095
Psyr_2096: Psyr_2096 - Sigma-70 region 2, at 2,444,019 to 2,444,609
_2096
Psyr_2097: Psyr_2097 - Thrombospondin type 3 repeat:OmpA/MotB:OmpF, at 2,444,715 to 2,445,749
_2097
Position (kb)
2443
2444 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2442.183 kb on + strand, within Psyr_2093 at 2442.410 kb on - strand, within Psyr_2093 at 2442.413 kb on + strand, within Psyr_2093 at 2442.413 kb on + strand, within Psyr_2093 at 2442.414 kb on - strand, within Psyr_2093 at 2442.414 kb on - strand, within Psyr_2093 at 2442.414 kb on - strand, within Psyr_2093 at 2442.593 kb on + strand, within Psyr_2093 at 2442.593 kb on + strand, within Psyr_2093 at 2442.593 kb on + strand, within Psyr_2093 at 2442.594 kb on - strand, within Psyr_2093 at 2442.594 kb on - strand, within Psyr_2093 at 2442.594 kb on - strand, within Psyr_2093 at 2442.610 kb on + strand, within Psyr_2093 at 2442.610 kb on + strand, within Psyr_2093 at 2442.611 kb on - strand, within Psyr_2093 at 2442.616 kb on + strand, within Psyr_2093 at 2442.616 kb on + strand, within Psyr_2093 at 2442.772 kb on - strand at 2442.773 kb on + strand at 2442.774 kb on - strand at 2442.792 kb on + strand at 2442.846 kb on + strand at 2442.846 kb on + strand at 2442.846 kb on + strand at 2442.846 kb on + strand at 2442.846 kb on + strand at 2442.847 kb on - strand at 2443.393 kb on + strand, within Psyr_2095 at 2443.393 kb on + strand, within Psyr_2095 at 2443.394 kb on - strand, within Psyr_2095 at 2443.395 kb on + strand, within Psyr_2095 at 2443.396 kb on - strand, within Psyr_2095 at 2443.466 kb on + strand, within Psyr_2095 at 2443.466 kb on + strand, within Psyr_2095 at 2443.467 kb on - strand, within Psyr_2095 at 2443.467 kb on - strand, within Psyr_2095 at 2443.479 kb on + strand, within Psyr_2095 at 2443.479 kb on + strand, within Psyr_2095 at 2443.479 kb on + strand, within Psyr_2095 at 2443.480 kb on - strand, within Psyr_2095 at 2443.574 kb on + strand, within Psyr_2095 at 2443.574 kb on + strand, within Psyr_2095 at 2443.625 kb on - strand, within Psyr_2095 at 2443.772 kb on + strand, within Psyr_2095 at 2443.772 kb on + strand at 2443.812 kb on + strand, within Psyr_2095 at 2443.923 kb on + strand at 2443.950 kb on + strand at 2443.955 kb on + strand at 2443.955 kb on + strand at 2444.049 kb on + strand at 2444.336 kb on + strand, within Psyr_2096 at 2444.443 kb on - strand, within Psyr_2096 at 2444.607 kb on + strand at 2444.607 kb on + strand at 2444.621 kb on + strand at 2444.621 kb on + strand at 2444.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,442,183 + Psyr_2093 0.40 +2.3 2,442,410 - Psyr_2093 0.63 -0.2 2,442,413 + Psyr_2093 0.64 -1.4 2,442,413 + Psyr_2093 0.64 +2.9 2,442,414 - Psyr_2093 0.64 +2.2 2,442,414 - Psyr_2093 0.64 +2.2 2,442,414 - Psyr_2093 0.64 +0.4 2,442,593 + Psyr_2093 0.82 -0.6 2,442,593 + Psyr_2093 0.82 -1.0 2,442,593 + Psyr_2093 0.82 +0.3 2,442,594 - Psyr_2093 0.82 +3.5 2,442,594 - Psyr_2093 0.82 -3.0 2,442,594 - Psyr_2093 0.82 +1.9 2,442,610 + Psyr_2093 0.83 +1.9 2,442,610 + Psyr_2093 0.83 +2.9 2,442,611 - Psyr_2093 0.83 +0.9 2,442,616 + Psyr_2093 0.84 +0.9 2,442,616 + Psyr_2093 0.84 +2.9 2,442,772 - -2.3 2,442,773 + +0.7 2,442,774 - -0.4 2,442,792 + -1.4 2,442,846 + -1.7 2,442,846 + -1.7 2,442,846 + +3.4 2,442,846 + -1.1 2,442,846 + +0.2 2,442,847 - -4.5 2,443,393 + Psyr_2095 0.34 +3.2 2,443,393 + Psyr_2095 0.34 -1.1 2,443,394 - Psyr_2095 0.34 +1.5 2,443,395 + Psyr_2095 0.34 +3.1 2,443,396 - Psyr_2095 0.34 -2.7 2,443,466 + Psyr_2095 0.43 -2.0 2,443,466 + Psyr_2095 0.43 +1.9 2,443,467 - Psyr_2095 0.43 -2.3 2,443,467 - Psyr_2095 0.43 -2.9 2,443,479 + Psyr_2095 0.44 +2.5 2,443,479 + Psyr_2095 0.44 -1.0 2,443,479 + Psyr_2095 0.44 -2.3 2,443,480 - Psyr_2095 0.45 -4.3 2,443,574 + Psyr_2095 0.56 -2.7 2,443,574 + Psyr_2095 0.56 -3.6 2,443,625 - Psyr_2095 0.62 -0.1 2,443,772 + Psyr_2095 0.80 -1.5 2,443,772 + +1.5 2,443,812 + Psyr_2095 0.85 +3.5 2,443,923 + +1.9 2,443,950 + +3.1 2,443,955 + -0.6 2,443,955 + +3.1 2,444,049 + +1.5 2,444,336 + Psyr_2096 0.54 -0.7 2,444,443 - Psyr_2096 0.72 -1.8 2,444,607 + +2.9 2,444,607 + +0.2 2,444,621 + +2.5 2,444,621 + -0.9 2,444,621 + +3.4
Or see this region's nucleotide sequence