Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2088 and Psyr_2089 are separated by 30 nucleotides Psyr_2089 and Psyr_2090 are separated by 221 nucleotides
Psyr_2088: Psyr_2088 - 2-methylaconitate cis-trans isomerase, at 2,435,367 to 2,436,557
_2088
Psyr_2089: Psyr_2089 - Protein of unknown function DUF299, at 2,436,588 to 2,437,406
_2089
Psyr_2090: Psyr_2090 - phosphoenolpyruvate synthase, at 2,437,628 to 2,440,003
_2090
Position (kb)
2436
2437
2438 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2435.622 kb on + strand, within Psyr_2088 at 2435.623 kb on - strand, within Psyr_2088 at 2436.228 kb on + strand, within Psyr_2088 at 2436.589 kb on + strand at 2436.590 kb on - strand at 2436.881 kb on - strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.979 kb on + strand, within Psyr_2089 at 2436.980 kb on - strand, within Psyr_2089 at 2436.980 kb on - strand, within Psyr_2089 at 2436.982 kb on + strand, within Psyr_2089 at 2436.983 kb on - strand, within Psyr_2089 at 2437.045 kb on + strand, within Psyr_2089 at 2437.046 kb on - strand, within Psyr_2089 at 2437.046 kb on - strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.087 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.257 kb on + strand, within Psyr_2089 at 2437.291 kb on + strand, within Psyr_2089 at 2437.291 kb on + strand, within Psyr_2089 at 2437.292 kb on - strand, within Psyr_2089 at 2437.292 kb on - strand, within Psyr_2089 at 2437.316 kb on + strand, within Psyr_2089 at 2437.316 kb on + strand, within Psyr_2089 at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.536 kb on - strand at 2437.536 kb on - strand at 2437.613 kb on - strand at 2437.633 kb on - strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.675 kb on - strand at 2437.675 kb on - strand at 2437.940 kb on - strand, within Psyr_2090 at 2438.337 kb on - strand, within Psyr_2090 at 2438.337 kb on - strand, within Psyr_2090
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,435,622 + Psyr_2088 0.21 +1.8 2,435,623 - Psyr_2088 0.21 +1.5 2,436,228 + Psyr_2088 0.72 -0.1 2,436,589 + -1.2 2,436,590 - +3.4 2,436,881 - Psyr_2089 0.36 +0.9 2,436,916 + Psyr_2089 0.40 -3.6 2,436,916 + Psyr_2089 0.40 +0.9 2,436,916 + Psyr_2089 0.40 +2.7 2,436,916 + Psyr_2089 0.40 +2.9 2,436,916 + Psyr_2089 0.40 -3.5 2,436,916 + Psyr_2089 0.40 -2.5 2,436,917 - Psyr_2089 0.40 -1.0 2,436,917 - Psyr_2089 0.40 -0.6 2,436,917 - Psyr_2089 0.40 -1.2 2,436,917 - Psyr_2089 0.40 +0.9 2,436,917 - Psyr_2089 0.40 -1.4 2,436,917 - Psyr_2089 0.40 +0.0 2,436,979 + Psyr_2089 0.48 -0.1 2,436,980 - Psyr_2089 0.48 -4.5 2,436,980 - Psyr_2089 0.48 +3.4 2,436,982 + Psyr_2089 0.48 -2.0 2,436,983 - Psyr_2089 0.48 +1.9 2,437,045 + Psyr_2089 0.56 -1.4 2,437,046 - Psyr_2089 0.56 -0.1 2,437,046 - Psyr_2089 0.56 -5.4 2,437,055 + Psyr_2089 0.57 -3.6 2,437,055 + Psyr_2089 0.57 -1.7 2,437,055 + Psyr_2089 0.57 -1.9 2,437,087 + Psyr_2089 0.61 -2.5 2,437,103 + Psyr_2089 0.63 +0.9 2,437,103 + Psyr_2089 0.63 +0.5 2,437,103 + Psyr_2089 0.63 -0.4 2,437,103 + Psyr_2089 0.63 -1.2 2,437,103 + Psyr_2089 0.63 +2.2 2,437,104 - Psyr_2089 0.63 -1.3 2,437,104 - Psyr_2089 0.63 +2.5 2,437,104 - Psyr_2089 0.63 +3.1 2,437,104 - Psyr_2089 0.63 -2.0 2,437,257 + Psyr_2089 0.82 -0.1 2,437,291 + Psyr_2089 0.86 -2.0 2,437,291 + Psyr_2089 0.86 -1.3 2,437,292 - Psyr_2089 0.86 +3.7 2,437,292 - Psyr_2089 0.86 -1.3 2,437,316 + Psyr_2089 0.89 -0.2 2,437,316 + Psyr_2089 0.89 +2.2 2,437,449 + +2.5 2,437,449 + -0.3 2,437,449 + +0.7 2,437,449 + -0.2 2,437,450 - +2.2 2,437,450 - +0.2 2,437,450 - +4.4 2,437,450 - -2.4 2,437,536 - -1.2 2,437,536 - +0.8 2,437,613 - -0.7 2,437,633 - +1.9 2,437,637 + -0.1 2,437,637 + +1.5 2,437,637 + -1.0 2,437,637 + +2.5 2,437,637 + +2.2 2,437,637 + +4.2 2,437,638 - +2.2 2,437,638 - +2.5 2,437,638 - -1.9 2,437,638 - +0.2 2,437,638 - +1.9 2,437,641 + -2.9 2,437,641 + -1.4 2,437,641 + -2.5 2,437,641 + +3.9 2,437,641 + +3.1 2,437,642 - -1.3 2,437,642 - +1.9 2,437,642 - +2.2 2,437,642 - -2.9 2,437,642 - +3.5 2,437,642 - -2.1 2,437,674 + +1.5 2,437,674 + +0.6 2,437,674 + +1.9 2,437,674 + -1.9 2,437,674 + -2.5 2,437,675 - -1.2 2,437,675 - -0.3 2,437,940 - Psyr_2090 0.13 -0.5 2,438,337 - Psyr_2090 0.30 -0.9 2,438,337 - Psyr_2090 0.30 -0.6
Or see this region's nucleotide sequence