Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2080

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2078 and Psyr_2079 are separated by 139 nucleotidesPsyr_2079 and Psyr_2080 are separated by 134 nucleotidesPsyr_2080 and Psyr_2081 are separated by 75 nucleotides Psyr_2078: Psyr_2078 - phosphoadenylylsulfate reductase (thioredoxin), at 2,423,281 to 2,424,015 _2078 Psyr_2079: Psyr_2079 - phosphoserine phosphatase / phosphoserine:homoserine phosphotransferase, at 2,424,155 to 2,424,772 _2079 Psyr_2080: Psyr_2080 - aminodeoxychorismate synthase, subunit I, at 2,424,907 to 2,426,250 _2080 Psyr_2081: Psyr_2081 - conserved hypothetical protein, at 2,426,326 to 2,427,306 _2081 Position (kb) 2424 2425 2426 2427Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 2424.029 kb on - strandat 2424.189 kb on + strandat 2424.189 kb on + strandat 2424.269 kb on + strand, within Psyr_2079at 2424.269 kb on + strand, within Psyr_2079at 2424.434 kb on + strand, within Psyr_2079at 2424.435 kb on - strand, within Psyr_2079at 2424.584 kb on + strand, within Psyr_2079at 2424.584 kb on + strand, within Psyr_2079at 2424.585 kb on - strand, within Psyr_2079at 2424.588 kb on - strand, within Psyr_2079at 2424.614 kb on + strand, within Psyr_2079at 2424.712 kb on + strandat 2424.712 kb on + strandat 2424.712 kb on + strandat 2424.712 kb on + strandat 2425.085 kb on + strand, within Psyr_2080at 2425.085 kb on + strand, within Psyr_2080at 2425.085 kb on + strand, within Psyr_2080at 2425.085 kb on + strand, within Psyr_2080at 2425.086 kb on - strand, within Psyr_2080at 2425.086 kb on - strand, within Psyr_2080at 2425.086 kb on - strand, within Psyr_2080at 2425.086 kb on - strand, within Psyr_2080at 2425.104 kb on - strand, within Psyr_2080at 2425.668 kb on - strand, within Psyr_2080at 2425.668 kb on - strand, within Psyr_2080at 2425.668 kb on - strand, within Psyr_2080at 2425.697 kb on + strand, within Psyr_2080at 2425.698 kb on - strand, within Psyr_2080at 2425.955 kb on + strand, within Psyr_2080at 2425.955 kb on + strand, within Psyr_2080at 2426.008 kb on + strand, within Psyr_2080at 2426.009 kb on - strand, within Psyr_2080at 2426.009 kb on - strand, within Psyr_2080at 2426.057 kb on + strand, within Psyr_2080at 2426.057 kb on + strand, within Psyr_2080at 2426.233 kb on + strandat 2426.288 kb on + strandat 2426.289 kb on - strandat 2426.289 kb on - strandat 2426.317 kb on + strandat 2426.318 kb on - strandat 2426.318 kb on - strandat 2426.318 kb on - strandat 2426.318 kb on - strandat 2426.573 kb on + strand, within Psyr_2081at 2426.574 kb on - strand, within Psyr_2081at 2426.610 kb on + strand, within Psyr_2081at 2426.611 kb on - strand, within Psyr_2081at 2426.838 kb on - strand, within Psyr_2081at 2426.838 kb on - strand, within Psyr_2081at 2426.915 kb on + strand, within Psyr_2081at 2427.128 kb on + strand, within Psyr_2081at 2427.128 kb on + strand, within Psyr_2081at 2427.128 kb on + strand, within Psyr_2081at 2427.128 kb on + strand, within Psyr_2081at 2427.128 kb on + strand, within Psyr_2081at 2427.129 kb on - strand, within Psyr_2081at 2427.129 kb on - strand, within Psyr_2081at 2427.129 kb on - strand, within Psyr_2081at 2427.129 kb on - strand, within Psyr_2081at 2427.218 kb on + strandat 2427.218 kb on + strandat 2427.219 kb on - strandat 2427.231 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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2,424,029 - +3.9
2,424,189 + +0.3
2,424,189 + +2.9
2,424,269 + Psyr_2079 0.18 +1.5
2,424,269 + Psyr_2079 0.18 -0.1
2,424,434 + Psyr_2079 0.45 -0.1
2,424,435 - Psyr_2079 0.45 -1.4
2,424,584 + Psyr_2079 0.69 -0.7
2,424,584 + Psyr_2079 0.69 +1.1
2,424,585 - Psyr_2079 0.70 +1.3
2,424,588 - Psyr_2079 0.70 +2.5
2,424,614 + Psyr_2079 0.74 +3.1
2,424,712 + -0.1
2,424,712 + +2.9
2,424,712 + +4.3
2,424,712 + +1.5
2,425,085 + Psyr_2080 0.13 -2.1
2,425,085 + Psyr_2080 0.13 +0.9
2,425,085 + Psyr_2080 0.13 -2.8
2,425,085 + Psyr_2080 0.13 +0.9
2,425,086 - Psyr_2080 0.13 -3.1
2,425,086 - Psyr_2080 0.13 +1.5
2,425,086 - Psyr_2080 0.13 -0.1
2,425,086 - Psyr_2080 0.13 -3.9
2,425,104 - Psyr_2080 0.15 +2.7
2,425,668 - Psyr_2080 0.57 -2.8
2,425,668 - Psyr_2080 0.57 -3.5
2,425,668 - Psyr_2080 0.57 -1.1
2,425,697 + Psyr_2080 0.59 -4.0
2,425,698 - Psyr_2080 0.59 -0.1
2,425,955 + Psyr_2080 0.78 +1.9
2,425,955 + Psyr_2080 0.78 +0.4
2,426,008 + Psyr_2080 0.82 -2.6
2,426,009 - Psyr_2080 0.82 -3.0
2,426,009 - Psyr_2080 0.82 +0.9
2,426,057 + Psyr_2080 0.86 -3.2
2,426,057 + Psyr_2080 0.86 -3.6
2,426,233 + -0.4
2,426,288 + +2.9
2,426,289 - +0.9
2,426,289 - +3.6
2,426,317 + -0.4
2,426,318 - -0.1
2,426,318 - -1.6
2,426,318 - +2.7
2,426,318 - -0.1
2,426,573 + Psyr_2081 0.25 -2.2
2,426,574 - Psyr_2081 0.25 -0.2
2,426,610 + Psyr_2081 0.29 -1.0
2,426,611 - Psyr_2081 0.29 -1.4
2,426,838 - Psyr_2081 0.52 -1.7
2,426,838 - Psyr_2081 0.52 -0.6
2,426,915 + Psyr_2081 0.60 +4.5
2,427,128 + Psyr_2081 0.82 +0.9
2,427,128 + Psyr_2081 0.82 +2.3
2,427,128 + Psyr_2081 0.82 +1.5
2,427,128 + Psyr_2081 0.82 -2.4
2,427,128 + Psyr_2081 0.82 -0.8
2,427,129 - Psyr_2081 0.82 -1.4
2,427,129 - Psyr_2081 0.82 -1.5
2,427,129 - Psyr_2081 0.82 +0.3
2,427,129 - Psyr_2081 0.82 +3.4
2,427,218 + -2.7
2,427,218 + +1.9
2,427,219 - -3.1
2,427,231 - +0.2

Or see this region's nucleotide sequence