Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2078 and Psyr_2079 are separated by 139 nucleotides Psyr_2079 and Psyr_2080 are separated by 134 nucleotides Psyr_2080 and Psyr_2081 are separated by 75 nucleotides
Psyr_2078: Psyr_2078 - phosphoadenylylsulfate reductase (thioredoxin), at 2,423,281 to 2,424,015
_2078
Psyr_2079: Psyr_2079 - phosphoserine phosphatase / phosphoserine:homoserine phosphotransferase, at 2,424,155 to 2,424,772
_2079
Psyr_2080: Psyr_2080 - aminodeoxychorismate synthase, subunit I, at 2,424,907 to 2,426,250
_2080
Psyr_2081: Psyr_2081 - conserved hypothetical protein, at 2,426,326 to 2,427,306
_2081
Position (kb)
2424
2425
2426
2427 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2424.029 kb on - strand at 2424.189 kb on + strand at 2424.189 kb on + strand at 2424.269 kb on + strand, within Psyr_2079 at 2424.269 kb on + strand, within Psyr_2079 at 2424.434 kb on + strand, within Psyr_2079 at 2424.435 kb on - strand, within Psyr_2079 at 2424.584 kb on + strand, within Psyr_2079 at 2424.584 kb on + strand, within Psyr_2079 at 2424.585 kb on - strand, within Psyr_2079 at 2424.588 kb on - strand, within Psyr_2079 at 2424.614 kb on + strand, within Psyr_2079 at 2424.712 kb on + strand at 2424.712 kb on + strand at 2424.712 kb on + strand at 2424.712 kb on + strand at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.104 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.697 kb on + strand, within Psyr_2080 at 2425.698 kb on - strand, within Psyr_2080 at 2425.955 kb on + strand, within Psyr_2080 at 2425.955 kb on + strand, within Psyr_2080 at 2426.008 kb on + strand, within Psyr_2080 at 2426.009 kb on - strand, within Psyr_2080 at 2426.009 kb on - strand, within Psyr_2080 at 2426.057 kb on + strand, within Psyr_2080 at 2426.057 kb on + strand, within Psyr_2080 at 2426.233 kb on + strand at 2426.288 kb on + strand at 2426.289 kb on - strand at 2426.289 kb on - strand at 2426.317 kb on + strand at 2426.318 kb on - strand at 2426.318 kb on - strand at 2426.318 kb on - strand at 2426.318 kb on - strand at 2426.573 kb on + strand, within Psyr_2081 at 2426.574 kb on - strand, within Psyr_2081 at 2426.610 kb on + strand, within Psyr_2081 at 2426.611 kb on - strand, within Psyr_2081 at 2426.838 kb on - strand, within Psyr_2081 at 2426.838 kb on - strand, within Psyr_2081 at 2426.915 kb on + strand, within Psyr_2081 at 2427.128 kb on + strand, within Psyr_2081 at 2427.128 kb on + strand, within Psyr_2081 at 2427.128 kb on + strand, within Psyr_2081 at 2427.128 kb on + strand, within Psyr_2081 at 2427.128 kb on + strand, within Psyr_2081 at 2427.129 kb on - strand, within Psyr_2081 at 2427.129 kb on - strand, within Psyr_2081 at 2427.129 kb on - strand, within Psyr_2081 at 2427.129 kb on - strand, within Psyr_2081 at 2427.218 kb on + strand at 2427.218 kb on + strand at 2427.219 kb on - strand at 2427.231 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,424,029 - +3.9 2,424,189 + +0.3 2,424,189 + +2.9 2,424,269 + Psyr_2079 0.18 +1.5 2,424,269 + Psyr_2079 0.18 -0.1 2,424,434 + Psyr_2079 0.45 -0.1 2,424,435 - Psyr_2079 0.45 -1.4 2,424,584 + Psyr_2079 0.69 -0.7 2,424,584 + Psyr_2079 0.69 +1.1 2,424,585 - Psyr_2079 0.70 +1.3 2,424,588 - Psyr_2079 0.70 +2.5 2,424,614 + Psyr_2079 0.74 +3.1 2,424,712 + -0.1 2,424,712 + +2.9 2,424,712 + +4.3 2,424,712 + +1.5 2,425,085 + Psyr_2080 0.13 -2.1 2,425,085 + Psyr_2080 0.13 +0.9 2,425,085 + Psyr_2080 0.13 -2.8 2,425,085 + Psyr_2080 0.13 +0.9 2,425,086 - Psyr_2080 0.13 -3.1 2,425,086 - Psyr_2080 0.13 +1.5 2,425,086 - Psyr_2080 0.13 -0.1 2,425,086 - Psyr_2080 0.13 -3.9 2,425,104 - Psyr_2080 0.15 +2.7 2,425,668 - Psyr_2080 0.57 -2.8 2,425,668 - Psyr_2080 0.57 -3.5 2,425,668 - Psyr_2080 0.57 -1.1 2,425,697 + Psyr_2080 0.59 -4.0 2,425,698 - Psyr_2080 0.59 -0.1 2,425,955 + Psyr_2080 0.78 +1.9 2,425,955 + Psyr_2080 0.78 +0.4 2,426,008 + Psyr_2080 0.82 -2.6 2,426,009 - Psyr_2080 0.82 -3.0 2,426,009 - Psyr_2080 0.82 +0.9 2,426,057 + Psyr_2080 0.86 -3.2 2,426,057 + Psyr_2080 0.86 -3.6 2,426,233 + -0.4 2,426,288 + +2.9 2,426,289 - +0.9 2,426,289 - +3.6 2,426,317 + -0.4 2,426,318 - -0.1 2,426,318 - -1.6 2,426,318 - +2.7 2,426,318 - -0.1 2,426,573 + Psyr_2081 0.25 -2.2 2,426,574 - Psyr_2081 0.25 -0.2 2,426,610 + Psyr_2081 0.29 -1.0 2,426,611 - Psyr_2081 0.29 -1.4 2,426,838 - Psyr_2081 0.52 -1.7 2,426,838 - Psyr_2081 0.52 -0.6 2,426,915 + Psyr_2081 0.60 +4.5 2,427,128 + Psyr_2081 0.82 +0.9 2,427,128 + Psyr_2081 0.82 +2.3 2,427,128 + Psyr_2081 0.82 +1.5 2,427,128 + Psyr_2081 0.82 -2.4 2,427,128 + Psyr_2081 0.82 -0.8 2,427,129 - Psyr_2081 0.82 -1.4 2,427,129 - Psyr_2081 0.82 -1.5 2,427,129 - Psyr_2081 0.82 +0.3 2,427,129 - Psyr_2081 0.82 +3.4 2,427,218 + -2.7 2,427,218 + +1.9 2,427,219 - -3.1 2,427,231 - +0.2
Or see this region's nucleotide sequence