Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2071 and Psyr_2072 are separated by 395 nucleotides Psyr_2072 and Psyr_2073 are separated by 86 nucleotides Psyr_2073 and Psyr_2074 are separated by 221 nucleotides
Psyr_2071: Psyr_2071 - outer membrane lipoprotein OprI, at 2,417,167 to 2,417,418
_2071
Psyr_2072: Psyr_2072 - conserved hypothetical protein, at 2,417,814 to 2,418,167
_2072
Psyr_2073: Psyr_2073 - 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase, at 2,418,254 to 2,419,342
_2073
Psyr_2074: Psyr_2074 - ketopantoate reductase, at 2,419,564 to 2,420,535
_2074
Position (kb)
2418
2419
2420 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2417.350 kb on + strand, within Psyr_2071 at 2417.351 kb on - strand, within Psyr_2071 at 2417.451 kb on + strand at 2417.452 kb on - strand at 2417.486 kb on - strand at 2417.486 kb on - strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.489 kb on + strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.490 kb on - strand at 2417.492 kb on + strand at 2417.493 kb on - strand at 2417.505 kb on + strand at 2417.505 kb on + strand at 2417.505 kb on + strand at 2417.506 kb on - strand at 2417.506 kb on - strand at 2417.506 kb on - strand at 2417.508 kb on + strand at 2417.569 kb on + strand at 2417.702 kb on + strand at 2417.723 kb on + strand at 2417.740 kb on + strand at 2417.741 kb on - strand at 2417.741 kb on - strand at 2417.743 kb on + strand at 2417.743 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.811 kb on + strand at 2417.812 kb on - strand at 2417.812 kb on - strand at 2417.812 kb on - strand at 2417.812 kb on - strand at 2417.956 kb on - strand at 2417.956 kb on - strand, within Psyr_2072 at 2417.956 kb on - strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.964 kb on + strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.965 kb on - strand, within Psyr_2072 at 2417.966 kb on + strand, within Psyr_2072 at 2417.966 kb on + strand, within Psyr_2072 at 2417.966 kb on + strand, within Psyr_2072 at 2417.967 kb on - strand, within Psyr_2072 at 2417.974 kb on + strand, within Psyr_2072 at 2417.975 kb on - strand, within Psyr_2072 at 2417.975 kb on - strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.111 kb on + strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.112 kb on - strand, within Psyr_2072 at 2418.123 kb on + strand, within Psyr_2072 at 2418.123 kb on + strand, within Psyr_2072 at 2418.123 kb on + strand, within Psyr_2072 at 2418.123 kb on + strand, within Psyr_2072 at 2418.176 kb on - strand at 2418.176 kb on - strand at 2418.195 kb on - strand at 2418.255 kb on + strand at 2418.255 kb on + strand at 2418.255 kb on + strand at 2418.256 kb on - strand at 2418.603 kb on + strand, within Psyr_2073 at 2418.603 kb on + strand, within Psyr_2073 at 2418.604 kb on - strand, within Psyr_2073 at 2418.604 kb on - strand, within Psyr_2073 at 2418.873 kb on + strand, within Psyr_2073 at 2419.047 kb on + strand, within Psyr_2073 at 2419.047 kb on + strand, within Psyr_2073 at 2419.066 kb on - strand, within Psyr_2073 at 2419.114 kb on - strand, within Psyr_2073 at 2419.448 kb on + strand at 2419.449 kb on - strand at 2419.452 kb on + strand at 2419.452 kb on + strand at 2419.452 kb on + strand at 2419.495 kb on + strand at 2419.495 kb on + strand at 2419.496 kb on - strand at 2419.527 kb on + strand at 2419.763 kb on - strand, within Psyr_2074 at 2419.946 kb on + strand, within Psyr_2074 at 2419.946 kb on + strand, within Psyr_2074 at 2419.947 kb on - strand, within Psyr_2074 at 2420.179 kb on + strand, within Psyr_2074 at 2420.179 kb on + strand, within Psyr_2074 at 2420.179 kb on + strand, within Psyr_2074 at 2420.180 kb on - strand, within Psyr_2074 at 2420.180 kb on - strand, within Psyr_2074 at 2420.180 kb on - strand, within Psyr_2074 at 2420.180 kb on - strand, within Psyr_2074 at 2420.180 kb on - strand, within Psyr_2074
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,417,350 + Psyr_2071 0.73 +1.9 2,417,351 - Psyr_2071 0.73 -0.9 2,417,451 + +2.5 2,417,452 - +1.5 2,417,486 - -2.9 2,417,486 - +2.5 2,417,489 + +0.1 2,417,489 + -2.6 2,417,489 + +0.9 2,417,489 + +2.9 2,417,489 + -0.4 2,417,489 + +3.9 2,417,489 + +3.3 2,417,490 - -0.3 2,417,490 - +1.9 2,417,490 - +0.5 2,417,490 - +4.3 2,417,490 - +2.9 2,417,490 - +1.2 2,417,490 - +4.5 2,417,490 - +3.4 2,417,490 - +3.5 2,417,492 + -3.9 2,417,493 - +0.2 2,417,505 + -2.4 2,417,505 + -0.1 2,417,505 + +1.5 2,417,506 - +3.5 2,417,506 - -0.1 2,417,506 - +1.5 2,417,508 + -2.1 2,417,569 + -0.1 2,417,702 + +2.8 2,417,723 + -0.5 2,417,740 + -0.1 2,417,741 - -0.0 2,417,741 - -0.7 2,417,743 + +2.3 2,417,743 + +2.8 2,417,811 + +1.3 2,417,811 + -0.8 2,417,811 + -2.9 2,417,811 + -0.5 2,417,811 + +1.0 2,417,811 + +2.8 2,417,811 + -0.7 2,417,812 - +3.1 2,417,812 - -1.9 2,417,812 - -0.6 2,417,812 - +4.5 2,417,956 - +2.3 2,417,956 - Psyr_2072 0.40 -1.6 2,417,956 - Psyr_2072 0.40 +3.5 2,417,964 + Psyr_2072 0.42 -0.9 2,417,964 + Psyr_2072 0.42 +3.0 2,417,964 + Psyr_2072 0.42 -1.3 2,417,964 + Psyr_2072 0.42 +1.5 2,417,964 + Psyr_2072 0.42 +1.1 2,417,964 + Psyr_2072 0.42 +4.0 2,417,964 + Psyr_2072 0.42 -0.7 2,417,964 + Psyr_2072 0.42 -1.1 2,417,965 - Psyr_2072 0.43 +4.0 2,417,965 - Psyr_2072 0.43 -2.3 2,417,965 - Psyr_2072 0.43 +3.8 2,417,965 - Psyr_2072 0.43 +1.0 2,417,965 - Psyr_2072 0.43 +1.5 2,417,965 - Psyr_2072 0.43 +2.0 2,417,965 - Psyr_2072 0.43 -3.2 2,417,965 - Psyr_2072 0.43 +3.7 2,417,965 - Psyr_2072 0.43 -2.0 2,417,966 + Psyr_2072 0.43 -1.5 2,417,966 + Psyr_2072 0.43 +1.4 2,417,966 + Psyr_2072 0.43 +4.5 2,417,967 - Psyr_2072 0.43 +4.1 2,417,974 + Psyr_2072 0.45 -0.2 2,417,975 - Psyr_2072 0.45 +1.5 2,417,975 - Psyr_2072 0.45 -1.4 2,418,111 + Psyr_2072 0.84 +1.7 2,418,111 + Psyr_2072 0.84 +2.3 2,418,111 + Psyr_2072 0.84 +2.3 2,418,111 + Psyr_2072 0.84 +1.0 2,418,111 + Psyr_2072 0.84 +0.4 2,418,111 + Psyr_2072 0.84 +0.4 2,418,112 - Psyr_2072 0.84 -0.2 2,418,112 - Psyr_2072 0.84 +1.0 2,418,112 - Psyr_2072 0.84 -3.4 2,418,112 - Psyr_2072 0.84 +4.1 2,418,112 - Psyr_2072 0.84 -4.2 2,418,112 - Psyr_2072 0.84 -0.2 2,418,112 - Psyr_2072 0.84 -0.5 2,418,123 + Psyr_2072 0.87 +0.2 2,418,123 + Psyr_2072 0.87 -1.8 2,418,123 + Psyr_2072 0.87 +4.3 2,418,123 + Psyr_2072 0.87 +2.3 2,418,176 - -1.2 2,418,176 - -0.4 2,418,195 - -0.1 2,418,255 + -2.5 2,418,255 + +3.5 2,418,255 + -1.3 2,418,256 - -1.2 2,418,603 + Psyr_2073 0.32 +3.4 2,418,603 + Psyr_2073 0.32 +1.7 2,418,604 - Psyr_2073 0.32 +2.0 2,418,604 - Psyr_2073 0.32 +2.3 2,418,873 + Psyr_2073 0.57 +2.8 2,419,047 + Psyr_2073 0.73 +2.0 2,419,047 + Psyr_2073 0.73 -1.7 2,419,066 - Psyr_2073 0.75 +3.5 2,419,114 - Psyr_2073 0.79 +3.3 2,419,448 + -0.4 2,419,449 - -1.6 2,419,452 + +0.4 2,419,452 + +2.5 2,419,452 + +2.0 2,419,495 + +1.0 2,419,495 + +1.2 2,419,496 - -1.5 2,419,527 + +3.8 2,419,763 - Psyr_2074 0.20 -3.2 2,419,946 + Psyr_2074 0.39 +2.0 2,419,946 + Psyr_2074 0.39 +4.1 2,419,947 - Psyr_2074 0.39 -0.8 2,420,179 + Psyr_2074 0.63 +0.1 2,420,179 + Psyr_2074 0.63 +1.0 2,420,179 + Psyr_2074 0.63 +2.3 2,420,180 - Psyr_2074 0.63 -1.6 2,420,180 - Psyr_2074 0.63 -1.5 2,420,180 - Psyr_2074 0.63 +1.0 2,420,180 - Psyr_2074 0.63 +0.0 2,420,180 - Psyr_2074 0.63 +4.4
Or see this region's nucleotide sequence