Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2060 and Psyr_2061 are separated by 180 nucleotides Psyr_2061 and Psyr_2062 are separated by 112 nucleotides Psyr_2062 and Psyr_2063 are separated by 244 nucleotides
Psyr_2060: Psyr_2060 - Sec-independent protein translocase TatD, at 2,406,820 to 2,407,641
_2060
Psyr_2061: Psyr_2061 - SLT, at 2,407,822 to 2,409,243
_2061
Psyr_2062: Psyr_2062 - DoxD-like family protein, at 2,409,356 to 2,409,790
_2062
Psyr_2063: Psyr_2063 - conserved hypothetical protein, at 2,410,035 to 2,410,343
_2063
Position (kb)
2407
2408
2409
2410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2406.822 kb on - strand at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2407.133 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.562 kb on + strand at 2407.647 kb on + strand at 2407.989 kb on + strand, within Psyr_2061 at 2407.990 kb on - strand, within Psyr_2061 at 2408.032 kb on + strand, within Psyr_2061 at 2408.032 kb on + strand, within Psyr_2061 at 2408.033 kb on - strand, within Psyr_2061 at 2408.458 kb on + strand, within Psyr_2061 at 2408.459 kb on - strand, within Psyr_2061 at 2408.560 kb on - strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.628 kb on + strand, within Psyr_2061 at 2408.629 kb on - strand, within Psyr_2061 at 2408.668 kb on + strand, within Psyr_2061 at 2408.669 kb on - strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.965 kb on + strand, within Psyr_2061 at 2408.965 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.172 kb on + strand at 2409.172 kb on + strand at 2409.172 kb on + strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.176 kb on - strand at 2409.176 kb on - strand at 2409.506 kb on + strand, within Psyr_2062 at 2409.506 kb on + strand, within Psyr_2062 at 2409.506 kb on + strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.916 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.924 kb on - strand at 2409.924 kb on - strand at 2409.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,406,822 - +0.3 2,406,925 + Psyr_2060 0.13 -2.1 2,406,925 + Psyr_2060 0.13 -1.4 2,406,925 + Psyr_2060 0.13 +2.8 2,406,925 + Psyr_2060 0.13 +2.8 2,407,133 + Psyr_2060 0.38 -0.4 2,407,337 + Psyr_2060 0.63 +4.0 2,407,337 + Psyr_2060 0.63 +3.1 2,407,337 + Psyr_2060 0.63 -1.1 2,407,562 + +3.0 2,407,647 + -0.8 2,407,989 + Psyr_2061 0.12 -3.6 2,407,990 - Psyr_2061 0.12 -0.0 2,408,032 + Psyr_2061 0.15 -1.0 2,408,032 + Psyr_2061 0.15 -1.2 2,408,033 - Psyr_2061 0.15 +0.2 2,408,458 + Psyr_2061 0.45 +0.3 2,408,459 - Psyr_2061 0.45 +0.5 2,408,560 - Psyr_2061 0.52 +2.5 2,408,626 + Psyr_2061 0.57 +2.3 2,408,626 + Psyr_2061 0.57 +3.3 2,408,626 + Psyr_2061 0.57 +0.8 2,408,626 + Psyr_2061 0.57 -1.4 2,408,627 - Psyr_2061 0.57 +2.0 2,408,627 - Psyr_2061 0.57 +0.2 2,408,627 - Psyr_2061 0.57 +2.0 2,408,627 - Psyr_2061 0.57 +3.5 2,408,627 - Psyr_2061 0.57 -3.2 2,408,628 + Psyr_2061 0.57 -0.7 2,408,629 - Psyr_2061 0.57 -1.6 2,408,668 + Psyr_2061 0.59 +1.5 2,408,669 - Psyr_2061 0.60 -1.7 2,408,680 + Psyr_2061 0.60 +2.8 2,408,680 + Psyr_2061 0.60 +2.8 2,408,680 + Psyr_2061 0.60 +1.0 2,408,680 + Psyr_2061 0.60 -2.0 2,408,680 + Psyr_2061 0.60 +0.1 2,408,681 - Psyr_2061 0.60 -0.4 2,408,681 - Psyr_2061 0.60 -2.0 2,408,681 - Psyr_2061 0.60 +2.0 2,408,681 - Psyr_2061 0.60 -1.6 2,408,681 - Psyr_2061 0.60 +0.8 2,408,681 - Psyr_2061 0.60 +0.2 2,408,681 - Psyr_2061 0.60 +3.5 2,408,681 - Psyr_2061 0.60 +1.0 2,408,965 + Psyr_2061 0.80 +1.4 2,408,965 + Psyr_2061 0.80 +2.3 2,409,022 + Psyr_2061 0.84 -0.1 2,409,022 + Psyr_2061 0.84 -1.3 2,409,022 + Psyr_2061 0.84 +4.0 2,409,023 - Psyr_2061 0.84 +2.5 2,409,023 - Psyr_2061 0.84 -1.2 2,409,023 - Psyr_2061 0.84 -0.2 2,409,172 + -0.0 2,409,172 + +2.5 2,409,172 + -3.1 2,409,173 - -0.0 2,409,173 - +3.5 2,409,173 - +1.0 2,409,173 - -0.6 2,409,175 + +0.1 2,409,175 + +2.5 2,409,175 + +2.0 2,409,175 + +2.8 2,409,175 + +2.8 2,409,176 - +0.1 2,409,176 - -0.0 2,409,506 + Psyr_2062 0.34 -0.0 2,409,506 + Psyr_2062 0.34 +2.0 2,409,506 + Psyr_2062 0.34 +3.5 2,409,507 - Psyr_2062 0.35 -0.5 2,409,507 - Psyr_2062 0.35 +1.0 2,409,507 - Psyr_2062 0.35 +1.0 2,409,507 - Psyr_2062 0.35 -1.5 2,409,507 - Psyr_2062 0.35 -0.3 2,409,916 + -0.8 2,409,923 + +5.0 2,409,923 + +1.5 2,409,923 + +3.9 2,409,923 + -0.5 2,409,923 + -0.8 2,409,924 - +2.8 2,409,924 - -0.8 2,409,964 - -1.5
Or see this region's nucleotide sequence