Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2024 and Psyr_2025 are separated by 62 nucleotides Psyr_2025 and Psyr_2026 are separated by 250 nucleotides Psyr_2026 and Psyr_2027 overlap by 4 nucleotides Psyr_2027 and Psyr_2028 are separated by 127 nucleotides
Psyr_2024: Psyr_2024 - Phosphohistidine phosphatase SixA, at 2,349,422 to 2,349,877
_2024
Psyr_2025: Psyr_2025 - Phenylacetic acid degradation-related protein, at 2,349,940 to 2,350,383
_2025
Psyr_2026: Psyr_2026 - conserved hypothetical protein, at 2,350,634 to 2,351,443
_2026
Psyr_2027: Psyr_2027 - epoxide hydrolase, Serine peptidase, MEROPS family S33, at 2,351,440 to 2,352,294
_2027
Psyr_2028: Psyr_2028 - conserved hypothetical protein, at 2,352,422 to 2,353,252
_2028
Position (kb)
2350
2351
2352 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2349.653 kb on - strand, within Psyr_2024 at 2349.714 kb on + strand, within Psyr_2024 at 2349.948 kb on - strand at 2349.948 kb on - strand at 2349.948 kb on - strand at 2350.078 kb on - strand, within Psyr_2025 at 2350.154 kb on - strand, within Psyr_2025 at 2350.177 kb on + strand, within Psyr_2025 at 2350.177 kb on + strand, within Psyr_2025 at 2350.177 kb on + strand, within Psyr_2025 at 2350.177 kb on + strand, within Psyr_2025 at 2350.178 kb on - strand, within Psyr_2025 at 2350.266 kb on - strand, within Psyr_2025 at 2350.266 kb on - strand, within Psyr_2025 at 2350.266 kb on - strand, within Psyr_2025 at 2350.266 kb on - strand, within Psyr_2025 at 2350.266 kb on - strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.283 kb on + strand, within Psyr_2025 at 2350.284 kb on - strand, within Psyr_2025 at 2350.284 kb on - strand, within Psyr_2025 at 2350.284 kb on - strand, within Psyr_2025 at 2350.284 kb on - strand, within Psyr_2025 at 2350.395 kb on + strand at 2350.395 kb on + strand at 2350.395 kb on + strand at 2350.396 kb on - strand at 2350.456 kb on + strand at 2350.456 kb on + strand at 2350.456 kb on + strand at 2350.457 kb on - strand at 2350.457 kb on - strand at 2350.458 kb on + strand at 2350.458 kb on + strand at 2350.458 kb on + strand at 2350.459 kb on - strand at 2350.459 kb on - strand at 2350.459 kb on - strand at 2350.459 kb on - strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.460 kb on + strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.461 kb on - strand at 2350.493 kb on + strand at 2350.494 kb on - strand at 2350.494 kb on - strand at 2350.495 kb on + strand at 2350.685 kb on + strand at 2350.685 kb on + strand at 2350.686 kb on - strand at 2350.686 kb on - strand at 2350.719 kb on - strand, within Psyr_2026 at 2351.114 kb on + strand, within Psyr_2026 at 2351.114 kb on + strand, within Psyr_2026 at 2351.190 kb on - strand, within Psyr_2026 at 2351.324 kb on + strand, within Psyr_2026 at 2351.325 kb on - strand, within Psyr_2026 at 2351.643 kb on + strand, within Psyr_2027 at 2351.663 kb on - strand, within Psyr_2027 at 2351.663 kb on - strand, within Psyr_2027 at 2351.677 kb on + strand, within Psyr_2027 at 2351.677 kb on + strand, within Psyr_2027 at 2351.677 kb on + strand, within Psyr_2027 at 2351.677 kb on + strand, within Psyr_2027 at 2351.677 kb on + strand at 2351.678 kb on - strand, within Psyr_2027 at 2352.030 kb on + strand, within Psyr_2027 at 2352.030 kb on + strand, within Psyr_2027 at 2352.031 kb on - strand, within Psyr_2027 at 2352.031 kb on - strand, within Psyr_2027 at 2352.031 kb on - strand, within Psyr_2027 at 2352.071 kb on + strand, within Psyr_2027 at 2352.071 kb on + strand, within Psyr_2027 at 2352.072 kb on - strand, within Psyr_2027 at 2352.078 kb on + strand, within Psyr_2027 at 2352.078 kb on + strand, within Psyr_2027 at 2352.078 kb on + strand, within Psyr_2027 at 2352.079 kb on - strand, within Psyr_2027 at 2352.079 kb on - strand, within Psyr_2027 at 2352.079 kb on - strand, within Psyr_2027 at 2352.142 kb on - strand, within Psyr_2027 at 2352.267 kb on + strand at 2352.267 kb on + strand at 2352.268 kb on - strand at 2352.394 kb on + strand at 2352.394 kb on + strand at 2352.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,349,653 - Psyr_2024 0.51 +0.5 2,349,714 + Psyr_2024 0.64 +2.6 2,349,948 - -0.9 2,349,948 - -0.0 2,349,948 - -1.5 2,350,078 - Psyr_2025 0.31 -0.0 2,350,154 - Psyr_2025 0.48 +2.6 2,350,177 + Psyr_2025 0.53 -0.0 2,350,177 + Psyr_2025 0.53 +1.6 2,350,177 + Psyr_2025 0.53 +1.0 2,350,177 + Psyr_2025 0.53 -0.7 2,350,178 - Psyr_2025 0.54 -1.3 2,350,266 - Psyr_2025 0.73 -1.8 2,350,266 - Psyr_2025 0.73 -0.4 2,350,266 - Psyr_2025 0.73 -0.2 2,350,266 - Psyr_2025 0.73 -2.6 2,350,266 - Psyr_2025 0.73 -0.5 2,350,283 + Psyr_2025 0.77 -0.0 2,350,283 + Psyr_2025 0.77 +3.0 2,350,283 + Psyr_2025 0.77 +1.0 2,350,283 + Psyr_2025 0.77 -1.9 2,350,283 + Psyr_2025 0.77 -1.8 2,350,283 + Psyr_2025 0.77 -1.3 2,350,284 - Psyr_2025 0.77 +3.3 2,350,284 - Psyr_2025 0.77 -1.2 2,350,284 - Psyr_2025 0.77 +2.0 2,350,284 - Psyr_2025 0.77 -1.6 2,350,395 + +3.3 2,350,395 + +1.0 2,350,395 + -2.9 2,350,396 - +0.3 2,350,456 + +0.5 2,350,456 + +0.8 2,350,456 + -0.9 2,350,457 - -1.1 2,350,457 - +3.3 2,350,458 + -0.6 2,350,458 + +0.2 2,350,458 + -0.3 2,350,459 - +1.0 2,350,459 - -3.6 2,350,459 - -3.8 2,350,459 - +0.4 2,350,460 + +0.5 2,350,460 + -2.8 2,350,460 + +3.3 2,350,460 + -1.3 2,350,460 + -0.2 2,350,460 + -1.3 2,350,460 + +1.3 2,350,460 + -0.9 2,350,460 + -2.7 2,350,461 - +1.6 2,350,461 - +3.4 2,350,461 - +2.8 2,350,461 - +1.2 2,350,461 - +3.3 2,350,461 - +2.3 2,350,461 - -1.6 2,350,461 - -1.1 2,350,493 + -0.7 2,350,494 - -1.2 2,350,494 - -0.6 2,350,495 + +4.6 2,350,685 + +0.1 2,350,685 + -0.8 2,350,686 - -1.3 2,350,686 - +2.6 2,350,719 - Psyr_2026 0.10 -0.0 2,351,114 + Psyr_2026 0.59 +2.0 2,351,114 + Psyr_2026 0.59 +3.6 2,351,190 - Psyr_2026 0.69 -0.0 2,351,324 + Psyr_2026 0.85 -2.3 2,351,325 - Psyr_2026 0.85 -0.6 2,351,643 + Psyr_2027 0.24 +3.8 2,351,663 - Psyr_2027 0.26 +0.5 2,351,663 - Psyr_2027 0.26 -3.7 2,351,677 + Psyr_2027 0.28 +0.7 2,351,677 + Psyr_2027 0.28 +3.3 2,351,677 + Psyr_2027 0.28 +1.1 2,351,677 + Psyr_2027 0.28 -0.9 2,351,677 + -0.0 2,351,678 - Psyr_2027 0.28 +3.2 2,352,030 + Psyr_2027 0.69 -1.6 2,352,030 + Psyr_2027 0.69 +3.7 2,352,031 - Psyr_2027 0.69 +2.6 2,352,031 - Psyr_2027 0.69 +2.8 2,352,031 - Psyr_2027 0.69 +0.7 2,352,071 + Psyr_2027 0.74 -1.5 2,352,071 + Psyr_2027 0.74 +2.0 2,352,072 - Psyr_2027 0.74 -0.0 2,352,078 + Psyr_2027 0.75 -0.8 2,352,078 + Psyr_2027 0.75 +4.2 2,352,078 + Psyr_2027 0.75 +0.4 2,352,079 - Psyr_2027 0.75 -2.4 2,352,079 - Psyr_2027 0.75 -0.5 2,352,079 - Psyr_2027 0.75 -2.3 2,352,142 - Psyr_2027 0.82 +2.4 2,352,267 + +1.0 2,352,267 + +3.0 2,352,268 - +1.0 2,352,394 + -3.6 2,352,394 + -1.4 2,352,395 - +0.8
Or see this region's nucleotide sequence