Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2012 and Psyr_2013 overlap by 1 nucleotides Psyr_2013 and Psyr_2014 are separated by 98 nucleotides Psyr_2014 and Psyr_2015 are separated by 301 nucleotides
Psyr_2012: Psyr_2012 - succinyl-CoA synthetase (ADP-forming) beta subunit, at 2,335,587 to 2,336,753
_2012
Psyr_2013: Psyr_2013 - succinyl-CoA synthetase (ADP-forming) alpha subunit, at 2,336,753 to 2,337,634
_2013
Psyr_2014: Psyr_2014 - conserved hypothetical protein, at 2,337,733 to 2,338,308
_2014
Psyr_2015: Psyr_2015 - Branched-chain amino acid transport system II carrier protein, at 2,338,610 to 2,339,923
_2015
Position (kb)
2337
2338
2339 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2337.734 kb on + strand at 2337.734 kb on + strand at 2337.734 kb on + strand at 2337.735 kb on - strand at 2337.735 kb on - strand at 2337.744 kb on + strand at 2337.779 kb on - strand at 2338.122 kb on + strand, within Psyr_2014 at 2338.122 kb on + strand, within Psyr_2014 at 2338.124 kb on + strand, within Psyr_2014 at 2338.124 kb on + strand, within Psyr_2014 at 2338.125 kb on - strand, within Psyr_2014 at 2338.125 kb on - strand, within Psyr_2014 at 2338.125 kb on - strand, within Psyr_2014 at 2338.125 kb on - strand, within Psyr_2014 at 2338.314 kb on + strand at 2338.315 kb on - strand at 2338.315 kb on - strand at 2338.315 kb on - strand at 2338.315 kb on - strand at 2338.315 kb on - strand at 2338.328 kb on + strand at 2338.346 kb on + strand at 2338.389 kb on - strand at 2338.521 kb on + strand at 2338.521 kb on + strand at 2338.522 kb on - strand at 2338.522 kb on - strand at 2338.522 kb on - strand at 2338.583 kb on + strand at 2338.583 kb on + strand at 2338.701 kb on + strand at 2338.702 kb on - strand at 2338.728 kb on + strand at 2338.728 kb on + strand at 2338.728 kb on + strand at 2338.728 kb on + strand at 2338.728 kb on + strand at 2338.728 kb on + strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.729 kb on - strand at 2338.874 kb on + strand, within Psyr_2015 at 2338.979 kb on + strand, within Psyr_2015 at 2338.991 kb on + strand, within Psyr_2015 at 2338.991 kb on + strand, within Psyr_2015 at 2338.991 kb on + strand, within Psyr_2015 at 2338.992 kb on - strand, within Psyr_2015 at 2338.992 kb on - strand, within Psyr_2015 at 2339.187 kb on - strand, within Psyr_2015
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,337,734 + +1.6 2,337,734 + -0.0 2,337,734 + +3.3 2,337,735 - -0.0 2,337,735 - +1.0 2,337,744 + +2.3 2,337,779 - +3.8 2,338,122 + Psyr_2014 0.68 +2.8 2,338,122 + Psyr_2014 0.68 -1.6 2,338,124 + Psyr_2014 0.68 -1.3 2,338,124 + Psyr_2014 0.68 -1.2 2,338,125 - Psyr_2014 0.68 +2.3 2,338,125 - Psyr_2014 0.68 -1.9 2,338,125 - Psyr_2014 0.68 -1.2 2,338,125 - Psyr_2014 0.68 -2.9 2,338,314 + +3.7 2,338,315 - -1.6 2,338,315 - -0.9 2,338,315 - -1.5 2,338,315 - -0.3 2,338,315 - +0.4 2,338,328 + -2.7 2,338,346 + +2.0 2,338,389 - -3.2 2,338,521 + +2.0 2,338,521 + -1.6 2,338,522 - +2.0 2,338,522 - +2.3 2,338,522 - +3.0 2,338,583 + +1.6 2,338,583 + -0.0 2,338,701 + -1.7 2,338,702 - +4.9 2,338,728 + +2.6 2,338,728 + +2.3 2,338,728 + -0.0 2,338,728 + +4.1 2,338,728 + +1.5 2,338,728 + +1.6 2,338,729 - +3.3 2,338,729 - -0.0 2,338,729 - +0.1 2,338,729 - +2.0 2,338,729 - -3.3 2,338,729 - +3.8 2,338,729 - +2.3 2,338,729 - +3.0 2,338,729 - +0.3 2,338,874 + Psyr_2015 0.20 -2.4 2,338,979 + Psyr_2015 0.28 -1.0 2,338,991 + Psyr_2015 0.29 -1.2 2,338,991 + Psyr_2015 0.29 +1.0 2,338,991 + Psyr_2015 0.29 -0.4 2,338,992 - Psyr_2015 0.29 -0.0 2,338,992 - Psyr_2015 0.29 +0.9 2,339,187 - Psyr_2015 0.44 -3.3
Or see this region's nucleotide sequence