Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1981 and Psyr_1982 are separated by 63 nucleotides Psyr_1982 and Psyr_1983 are separated by 145 nucleotides
Psyr_1981: Psyr_1981 - PAS:GGDEF, at 2,299,450 to 2,301,642
_1981
Psyr_1982: Psyr_1982 - transcriptional regulator, LysR family, at 2,301,706 to 2,302,578
_1982
Psyr_1983: Psyr_1983 - 3-isopropylmalate dehydratase, large subunit, at 2,302,724 to 2,304,148
_1983
Position (kb)
2301
2302
2303 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2300.884 kb on + strand, within Psyr_1981 at 2300.884 kb on + strand, within Psyr_1981 at 2300.886 kb on + strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.086 kb on + strand, within Psyr_1981 at 2301.305 kb on + strand, within Psyr_1981 at 2301.350 kb on + strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.704 kb on - strand at 2301.782 kb on + strand at 2301.783 kb on - strand at 2301.783 kb on - strand at 2301.787 kb on + strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.805 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.907 kb on - strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.979 kb on - strand, within Psyr_1982 at 2302.021 kb on - strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.141 kb on - strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.373 kb on + strand, within Psyr_1982 at 2302.374 kb on - strand, within Psyr_1982 at 2302.638 kb on + strand at 2302.639 kb on - strand at 2302.694 kb on + strand at 2302.695 kb on - strand at 2302.695 kb on - strand at 2302.695 kb on - strand at 2302.716 kb on + strand at 2302.802 kb on + strand at 2302.802 kb on + strand at 2302.802 kb on + strand at 2302.802 kb on + strand at 2302.802 kb on + strand at 2302.802 kb on + strand at 2302.803 kb on - strand at 2302.803 kb on - strand at 2302.816 kb on + strand at 2302.816 kb on + strand at 2302.816 kb on + strand at 2302.817 kb on - strand at 2302.817 kb on - strand at 2302.817 kb on - strand at 2302.817 kb on - strand at 2303.135 kb on - strand, within Psyr_1983 at 2303.462 kb on + strand, within Psyr_1983 at 2303.462 kb on + strand, within Psyr_1983
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,300,884 + Psyr_1981 0.65 -0.5 2,300,884 + Psyr_1981 0.65 +3.8 2,300,886 + Psyr_1981 0.65 -0.0 2,300,887 - Psyr_1981 0.66 -3.3 2,300,887 - Psyr_1981 0.66 -3.1 2,301,021 + Psyr_1981 0.72 -2.0 2,301,021 + Psyr_1981 0.72 +2.6 2,301,021 + Psyr_1981 0.72 -2.1 2,301,086 + Psyr_1981 0.75 +1.0 2,301,305 + Psyr_1981 0.85 +3.2 2,301,350 + Psyr_1981 0.87 +3.0 2,301,351 - Psyr_1981 0.87 -1.8 2,301,351 - Psyr_1981 0.87 +0.2 2,301,704 - -0.1 2,301,782 + -0.0 2,301,783 - +0.6 2,301,783 - -0.8 2,301,787 + +3.4 2,301,788 - -1.8 2,301,788 - +2.3 2,301,788 - -4.7 2,301,805 + Psyr_1982 0.11 +1.1 2,301,906 + Psyr_1982 0.23 -1.8 2,301,906 + Psyr_1982 0.23 -1.3 2,301,906 + Psyr_1982 0.23 -1.2 2,301,907 - Psyr_1982 0.23 +0.1 2,301,908 + Psyr_1982 0.23 -4.0 2,301,908 + Psyr_1982 0.23 -2.6 2,301,908 + Psyr_1982 0.23 +3.3 2,301,908 + Psyr_1982 0.23 -0.2 2,301,908 + Psyr_1982 0.23 +4.2 2,301,908 + Psyr_1982 0.23 +1.0 2,301,908 + Psyr_1982 0.23 +4.1 2,301,908 + Psyr_1982 0.23 -1.8 2,301,908 + Psyr_1982 0.23 -2.7 2,301,908 + Psyr_1982 0.23 -0.6 2,301,908 + Psyr_1982 0.23 +4.7 2,301,909 - Psyr_1982 0.23 -0.0 2,301,909 - Psyr_1982 0.23 +0.3 2,301,909 - Psyr_1982 0.23 -0.0 2,301,909 - Psyr_1982 0.23 +3.0 2,301,909 - Psyr_1982 0.23 +2.6 2,301,909 - Psyr_1982 0.23 -1.3 2,301,909 - Psyr_1982 0.23 -0.0 2,301,909 - Psyr_1982 0.23 -0.0 2,301,909 - Psyr_1982 0.23 +4.4 2,301,909 - Psyr_1982 0.23 -2.2 2,301,909 - Psyr_1982 0.23 -0.2 2,301,909 - Psyr_1982 0.23 -3.5 2,301,909 - Psyr_1982 0.23 +0.7 2,301,909 - Psyr_1982 0.23 -2.3 2,301,909 - Psyr_1982 0.23 -3.6 2,301,909 - Psyr_1982 0.23 -1.4 2,301,979 - Psyr_1982 0.31 +0.2 2,302,021 - Psyr_1982 0.36 -1.2 2,302,140 + Psyr_1982 0.50 +1.0 2,302,140 + Psyr_1982 0.50 -0.9 2,302,141 - Psyr_1982 0.50 -1.4 2,302,252 + Psyr_1982 0.63 +0.3 2,302,252 + Psyr_1982 0.63 +2.0 2,302,373 + Psyr_1982 0.76 -1.2 2,302,374 - Psyr_1982 0.77 -0.0 2,302,638 + -1.8 2,302,639 - -0.0 2,302,694 + +0.1 2,302,695 - +2.3 2,302,695 - -0.7 2,302,695 - +3.2 2,302,716 + +3.2 2,302,802 + -0.8 2,302,802 + +1.4 2,302,802 + -0.6 2,302,802 + -1.7 2,302,802 + -1.7 2,302,802 + -1.4 2,302,803 - -0.0 2,302,803 - +0.5 2,302,816 + -3.2 2,302,816 + -0.1 2,302,816 + +2.6 2,302,817 - +0.4 2,302,817 - +2.0 2,302,817 - -1.7 2,302,817 - +2.6 2,303,135 - Psyr_1983 0.29 +1.1 2,303,462 + Psyr_1983 0.52 -0.8 2,303,462 + Psyr_1983 0.52 +3.0
Or see this region's nucleotide sequence