Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1908 and Psyr_1909 are separated by 103 nucleotides Psyr_1909 and Psyr_1910 are separated by 198 nucleotides Psyr_1910 and Psyr_1911 are separated by 88 nucleotides Psyr_1911 and Psyr_1912 are separated by 267 nucleotides
Psyr_1908: Psyr_1908 - conserved hypothetical protein, at 2,183,680 to 2,183,949
_1908
Psyr_1909: Psyr_1909 - conserved hypothetical protein, at 2,184,053 to 2,184,661
_1909
Psyr_1910: Psyr_1910 - VacJ-like lipoprotein, at 2,184,860 to 2,185,561
_1910
Psyr_1911: Psyr_1911 - conserved hypothetical protein, at 2,185,650 to 2,185,949
_1911
Psyr_1912: Psyr_1912 - Response regulator receiver:Stage II sporulation E, at 2,186,217 to 2,187,401
_1912
Position (kb)
2184
2185
2186 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2183.968 kb on + strand at 2183.968 kb on + strand at 2183.968 kb on + strand at 2183.969 kb on - strand at 2183.969 kb on - strand at 2183.969 kb on - strand at 2183.999 kb on + strand at 2183.999 kb on + strand at 2184.000 kb on - strand at 2184.023 kb on + strand at 2184.024 kb on - strand at 2184.024 kb on - strand at 2184.051 kb on + strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.317 kb on + strand, within Psyr_1909 at 2184.767 kb on + strand at 2184.786 kb on + strand at 2184.786 kb on + strand at 2184.787 kb on - strand at 2184.787 kb on - strand at 2184.788 kb on + strand at 2184.788 kb on + strand at 2184.788 kb on + strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.800 kb on + strand at 2184.829 kb on + strand at 2184.859 kb on + strand at 2184.859 kb on + strand at 2184.860 kb on - strand at 2184.860 kb on - strand at 2185.017 kb on - strand, within Psyr_1910 at 2185.017 kb on - strand, within Psyr_1910 at 2185.044 kb on - strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.333 kb on - strand, within Psyr_1910 at 2185.333 kb on - strand, within Psyr_1910 at 2185.364 kb on + strand, within Psyr_1910 at 2185.365 kb on - strand, within Psyr_1910 at 2185.484 kb on + strand, within Psyr_1910 at 2185.506 kb on - strand at 2185.567 kb on - strand at 2185.612 kb on + strand at 2185.613 kb on - strand at 2185.616 kb on - strand at 2185.909 kb on + strand, within Psyr_1911 at 2185.909 kb on + strand, within Psyr_1911 at 2185.909 kb on + strand, within Psyr_1911 at 2185.909 kb on + strand, within Psyr_1911 at 2185.909 kb on + strand, within Psyr_1911 at 2185.910 kb on - strand, within Psyr_1911 at 2185.910 kb on - strand, within Psyr_1911 at 2185.951 kb on + strand at 2185.951 kb on + strand at 2185.952 kb on - strand at 2185.966 kb on + strand at 2185.966 kb on + strand at 2185.990 kb on + strand at 2185.990 kb on + strand at 2186.012 kb on + strand at 2186.013 kb on - strand at 2186.109 kb on + strand at 2186.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,183,968 + -1.7 2,183,968 + +3.2 2,183,968 + +0.1 2,183,969 - -1.6 2,183,969 - -1.8 2,183,969 - +2.3 2,183,999 + -2.5 2,183,999 + +1.6 2,184,000 - -3.7 2,184,023 + +2.8 2,184,024 - +2.3 2,184,024 - -3.5 2,184,051 + +2.3 2,184,052 - +2.0 2,184,052 - -1.7 2,184,052 - +2.3 2,184,052 - -2.1 2,184,052 - -2.2 2,184,052 - +0.0 2,184,317 + Psyr_1909 0.43 -0.5 2,184,767 + -1.6 2,184,786 + +0.0 2,184,786 + -0.2 2,184,787 - -1.2 2,184,787 - -1.0 2,184,788 + -1.2 2,184,788 + +1.0 2,184,788 + +0.3 2,184,789 - -4.5 2,184,789 - +2.6 2,184,789 - -1.4 2,184,789 - -0.7 2,184,800 + -1.4 2,184,829 + +0.0 2,184,859 + -2.5 2,184,859 + -2.9 2,184,860 - -3.5 2,184,860 - +1.6 2,185,017 - Psyr_1910 0.22 -3.1 2,185,017 - Psyr_1910 0.22 +1.0 2,185,044 - Psyr_1910 0.26 +1.6 2,185,049 + Psyr_1910 0.27 -0.6 2,185,049 + Psyr_1910 0.27 -2.3 2,185,049 + Psyr_1910 0.27 +0.0 2,185,049 + Psyr_1910 0.27 -3.5 2,185,049 + Psyr_1910 0.27 -1.6 2,185,050 - Psyr_1910 0.27 -3.1 2,185,050 - Psyr_1910 0.27 +0.0 2,185,050 - Psyr_1910 0.27 -5.0 2,185,050 - Psyr_1910 0.27 -3.5 2,185,050 - Psyr_1910 0.27 -0.5 2,185,050 - Psyr_1910 0.27 +1.0 2,185,332 + Psyr_1910 0.67 -3.5 2,185,332 + Psyr_1910 0.67 -3.5 2,185,332 + Psyr_1910 0.67 +1.6 2,185,332 + Psyr_1910 0.67 +1.0 2,185,332 + Psyr_1910 0.67 +0.0 2,185,333 - Psyr_1910 0.67 -3.3 2,185,333 - Psyr_1910 0.67 -2.5 2,185,364 + Psyr_1910 0.72 -0.2 2,185,365 - Psyr_1910 0.72 -2.2 2,185,484 + Psyr_1910 0.89 +0.0 2,185,506 - +2.8 2,185,567 - -1.7 2,185,612 + +0.4 2,185,613 - +0.0 2,185,616 - -2.8 2,185,909 + Psyr_1911 0.86 -2.0 2,185,909 + Psyr_1911 0.86 -1.6 2,185,909 + Psyr_1911 0.86 +1.9 2,185,909 + Psyr_1911 0.86 -1.0 2,185,909 + Psyr_1911 0.86 -3.0 2,185,910 - Psyr_1911 0.87 +2.3 2,185,910 - Psyr_1911 0.87 -2.5 2,185,951 + -0.6 2,185,951 + -0.6 2,185,952 - +1.0 2,185,966 + +1.0 2,185,966 + +3.8 2,185,990 + -1.5 2,185,990 + -1.8 2,186,012 + +4.0 2,186,013 - +1.0 2,186,109 + +2.3 2,186,109 + -1.5
Or see this region's nucleotide sequence