Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1847 and Psyr_1848 are separated by 45 nucleotides Psyr_1848 and Psyr_1849 are separated by 148 nucleotides
Psyr_1847: Psyr_1847 - Smr protein/MutS2 C-terminal, at 2,125,940 to 2,126,497
_1847
Psyr_1848: Psyr_1848 - conserved hypothetical protein, at 2,126,543 to 2,126,860
_1848
Psyr_1849: Psyr_1849 - Isochorismatase hydrolase, at 2,127,009 to 2,127,599
_1849
Position (kb)
2126
2127 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2125.603 kb on - strand at 2125.614 kb on + strand at 2125.615 kb on - strand at 2125.703 kb on - strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.752 kb on - strand at 2125.752 kb on - strand at 2125.941 kb on + strand at 2125.983 kb on + strand at 2125.983 kb on + strand at 2125.984 kb on - strand at 2125.984 kb on - strand at 2126.135 kb on + strand, within Psyr_1847 at 2126.135 kb on + strand, within Psyr_1847 at 2126.575 kb on + strand, within Psyr_1848 at 2126.575 kb on + strand, within Psyr_1848 at 2126.575 kb on + strand, within Psyr_1848 at 2126.576 kb on - strand, within Psyr_1848 at 2126.576 kb on - strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.878 kb on + strand at 2126.879 kb on - strand at 2127.164 kb on - strand, within Psyr_1849 at 2127.194 kb on + strand, within Psyr_1849
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,125,603 - +1.6 2,125,614 + +1.5 2,125,615 - +0.8 2,125,703 - -0.6 2,125,751 + +3.0 2,125,751 + +2.8 2,125,751 + -1.4 2,125,751 + -1.2 2,125,751 + +0.5 2,125,751 + +3.6 2,125,752 - -1.2 2,125,752 - +4.2 2,125,941 + +2.8 2,125,983 + +3.6 2,125,983 + +2.8 2,125,984 - -4.5 2,125,984 - -1.9 2,126,135 + Psyr_1847 0.35 +2.3 2,126,135 + Psyr_1847 0.35 +3.0 2,126,575 + Psyr_1848 0.10 +1.0 2,126,575 + Psyr_1848 0.10 +1.6 2,126,575 + Psyr_1848 0.10 -2.9 2,126,576 - Psyr_1848 0.10 -3.4 2,126,576 - Psyr_1848 0.10 +2.0 2,126,634 + Psyr_1848 0.29 -2.4 2,126,634 + Psyr_1848 0.29 +0.2 2,126,634 + Psyr_1848 0.29 +3.2 2,126,634 + Psyr_1848 0.29 +3.9 2,126,635 - Psyr_1848 0.29 +2.0 2,126,635 - Psyr_1848 0.29 +4.1 2,126,635 - Psyr_1848 0.29 -1.4 2,126,635 - Psyr_1848 0.29 +1.7 2,126,635 - Psyr_1848 0.29 +3.0 2,126,635 - Psyr_1848 0.29 +4.0 2,126,635 - Psyr_1848 0.29 +0.3 2,126,878 + +4.5 2,126,879 - -0.6 2,127,164 - Psyr_1849 0.26 +3.9 2,127,194 + Psyr_1849 0.31 -1.9
Or see this region's nucleotide sequence