Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1823 and Psyr_1824 are separated by 93 nucleotides Psyr_1824 and Psyr_1825 are separated by 100 nucleotides
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Psyr_1824: Psyr_1824 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,104,950 to 2,105,744
_1824
Psyr_1825: Psyr_1825 - DNA polymerase III, tau subunit, at 2,105,845 to 2,108,055
_1825
Position (kb)
2104
2105
2106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2103.967 kb on + strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2104.244 kb on - strand, within Psyr_1823 at 2104.258 kb on + strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.642 kb on + strand, within Psyr_1823 at 2104.643 kb on - strand, within Psyr_1823 at 2104.753 kb on + strand at 2104.753 kb on + strand at 2104.753 kb on + strand at 2104.865 kb on - strand at 2104.918 kb on + strand at 2104.918 kb on + strand at 2104.918 kb on + strand at 2104.919 kb on - strand at 2104.927 kb on - strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.973 kb on - strand at 2104.973 kb on - strand at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.282 kb on - strand, within Psyr_1824 at 2105.398 kb on + strand, within Psyr_1824 at 2105.399 kb on - strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.480 kb on + strand, within Psyr_1824 at 2105.670 kb on + strand at 2105.671 kb on - strand at 2105.724 kb on - strand at 2105.756 kb on - strand at 2105.756 kb on - strand at 2105.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,103,967 + Psyr_1823 0.47 +2.8 2,103,968 - Psyr_1823 0.47 +3.8 2,103,968 - Psyr_1823 0.47 +2.0 2,103,968 - Psyr_1823 0.47 -1.5 2,104,244 - Psyr_1823 0.64 -1.7 2,104,258 + Psyr_1823 0.64 -3.2 2,104,259 - Psyr_1823 0.65 +2.3 2,104,259 - Psyr_1823 0.65 +2.0 2,104,259 - Psyr_1823 0.65 +3.8 2,104,642 + Psyr_1823 0.87 -0.2 2,104,643 - Psyr_1823 0.87 +2.0 2,104,753 + +3.5 2,104,753 + -2.2 2,104,753 + +2.8 2,104,865 - +3.3 2,104,918 + -1.5 2,104,918 + -1.0 2,104,918 + +0.0 2,104,919 - +0.8 2,104,927 - +3.0 2,104,972 + +1.0 2,104,972 + -0.4 2,104,972 + +2.0 2,104,972 + -3.8 2,104,972 + +4.1 2,104,973 - +2.8 2,104,973 - -1.9 2,105,174 - Psyr_1824 0.28 -2.6 2,105,174 - Psyr_1824 0.28 +0.0 2,105,174 - Psyr_1824 0.28 -3.0 2,105,174 - Psyr_1824 0.28 -1.2 2,105,174 - Psyr_1824 0.28 +1.6 2,105,282 - Psyr_1824 0.42 +1.0 2,105,398 + Psyr_1824 0.56 -0.9 2,105,399 - Psyr_1824 0.56 -1.4 2,105,438 - Psyr_1824 0.61 +0.0 2,105,438 - Psyr_1824 0.61 +0.0 2,105,480 + Psyr_1824 0.67 -1.4 2,105,670 + -4.6 2,105,671 - -1.7 2,105,724 - +1.3 2,105,756 - -2.7 2,105,756 - -0.2 2,105,765 - +3.8
Or see this region's nucleotide sequence