Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1795 and Psyr_1796 are separated by 43 nucleotides Psyr_1796 and Psyr_1797 are separated by 155 nucleotides Psyr_1797 and Psyr_1798 are separated by 151 nucleotides
Psyr_1795: Psyr_1795 - Taurine dioxygenase, at 2,067,660 to 2,068,529
_1795
Psyr_1796: Psyr_1796 - GCN5-related N-acetyltransferase, at 2,068,573 to 2,069,088
_1796
Psyr_1797: Psyr_1797 - conserved hypothetical protein, at 2,069,244 to 2,070,371
_1797
Psyr_1798: Psyr_1798 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 2,070,523 to 2,072,568
_1798
Position (kb)
2069
2070
2071 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2068.352 kb on + strand, within Psyr_1795 at 2068.352 kb on + strand, within Psyr_1795 at 2068.353 kb on - strand, within Psyr_1795 at 2068.467 kb on + strand at 2068.468 kb on - strand at 2068.468 kb on - strand at 2068.468 kb on - strand at 2068.592 kb on + strand at 2068.592 kb on + strand at 2068.592 kb on + strand at 2068.592 kb on + strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.593 kb on - strand at 2068.594 kb on + strand at 2068.594 kb on + strand at 2068.594 kb on + strand at 2068.595 kb on - strand at 2068.953 kb on + strand, within Psyr_1796 at 2068.985 kb on - strand, within Psyr_1796 at 2068.985 kb on - strand, within Psyr_1796 at 2069.077 kb on + strand at 2069.078 kb on - strand at 2069.078 kb on - strand at 2069.078 kb on - strand at 2069.078 kb on - strand at 2069.129 kb on + strand at 2069.154 kb on + strand at 2069.155 kb on - strand at 2069.280 kb on - strand at 2069.280 kb on - strand at 2069.481 kb on - strand, within Psyr_1797 at 2069.481 kb on - strand, within Psyr_1797 at 2069.553 kb on + strand, within Psyr_1797 at 2069.554 kb on - strand, within Psyr_1797 at 2069.920 kb on + strand, within Psyr_1797 at 2069.920 kb on + strand, within Psyr_1797 at 2069.921 kb on - strand, within Psyr_1797 at 2069.921 kb on - strand, within Psyr_1797 at 2069.921 kb on - strand, within Psyr_1797 at 2070.114 kb on + strand, within Psyr_1797 at 2070.114 kb on + strand, within Psyr_1797 at 2070.114 kb on + strand, within Psyr_1797 at 2070.114 kb on + strand, within Psyr_1797 at 2070.114 kb on + strand, within Psyr_1797 at 2070.115 kb on - strand, within Psyr_1797 at 2070.115 kb on - strand, within Psyr_1797 at 2070.362 kb on + strand at 2070.484 kb on + strand at 2070.485 kb on - strand at 2070.489 kb on - strand at 2070.496 kb on - strand at 2070.517 kb on + strand at 2070.572 kb on + strand at 2070.572 kb on + strand at 2070.572 kb on + strand at 2070.573 kb on - strand at 2070.573 kb on - strand at 2070.573 kb on - strand at 2070.577 kb on - strand at 2070.638 kb on + strand at 2070.702 kb on + strand at 2070.838 kb on + strand, within Psyr_1798 at 2070.874 kb on + strand, within Psyr_1798 at 2071.249 kb on + strand, within Psyr_1798 at 2071.249 kb on + strand, within Psyr_1798 at 2071.249 kb on + strand, within Psyr_1798 at 2071.249 kb on + strand, within Psyr_1798 at 2071.249 kb on + strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.250 kb on - strand, within Psyr_1798 at 2071.251 kb on + strand, within Psyr_1798 at 2071.354 kb on + strand, within Psyr_1798 at 2071.355 kb on - strand, within Psyr_1798
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,068,352 + Psyr_1795 0.80 +0.2 2,068,352 + Psyr_1795 0.80 +3.7 2,068,353 - Psyr_1795 0.80 -1.0 2,068,467 + +3.3 2,068,468 - -2.8 2,068,468 - +1.6 2,068,468 - +3.6 2,068,592 + -0.4 2,068,592 + -0.5 2,068,592 + +2.0 2,068,592 + +1.0 2,068,593 - +1.0 2,068,593 - -0.6 2,068,593 - -0.3 2,068,593 - +3.8 2,068,593 - -0.4 2,068,593 - +3.7 2,068,593 - +1.6 2,068,593 - +0.0 2,068,593 - +2.6 2,068,593 - +0.0 2,068,594 + +0.5 2,068,594 + -1.5 2,068,594 + +0.0 2,068,595 - +3.3 2,068,953 + Psyr_1796 0.74 -1.0 2,068,985 - Psyr_1796 0.80 +3.9 2,068,985 - Psyr_1796 0.80 -0.8 2,069,077 + -2.2 2,069,078 - +2.3 2,069,078 - -0.6 2,069,078 - -0.9 2,069,078 - +4.4 2,069,129 + -0.1 2,069,154 + +0.7 2,069,155 - -0.3 2,069,280 - +0.4 2,069,280 - -0.5 2,069,481 - Psyr_1797 0.21 -4.2 2,069,481 - Psyr_1797 0.21 +0.0 2,069,553 + Psyr_1797 0.27 -1.6 2,069,554 - Psyr_1797 0.27 +1.9 2,069,920 + Psyr_1797 0.60 +1.6 2,069,920 + Psyr_1797 0.60 -3.5 2,069,921 - Psyr_1797 0.60 +3.7 2,069,921 - Psyr_1797 0.60 +3.3 2,069,921 - Psyr_1797 0.60 +3.0 2,070,114 + Psyr_1797 0.77 +2.3 2,070,114 + Psyr_1797 0.77 -1.8 2,070,114 + Psyr_1797 0.77 +3.8 2,070,114 + Psyr_1797 0.77 +4.6 2,070,114 + Psyr_1797 0.77 -1.7 2,070,115 - Psyr_1797 0.77 -1.5 2,070,115 - Psyr_1797 0.77 -0.8 2,070,362 + -1.4 2,070,484 + -1.7 2,070,485 - -1.9 2,070,489 - +2.3 2,070,496 - -1.1 2,070,517 + -0.6 2,070,572 + +0.2 2,070,572 + -0.6 2,070,572 + +0.3 2,070,573 - -3.2 2,070,573 - +2.3 2,070,573 - -0.8 2,070,577 - -0.9 2,070,638 + -1.0 2,070,702 + +2.8 2,070,838 + Psyr_1798 0.15 -1.1 2,070,874 + Psyr_1798 0.17 -0.6 2,071,249 + Psyr_1798 0.35 +2.8 2,071,249 + Psyr_1798 0.35 -2.0 2,071,249 + Psyr_1798 0.35 +3.2 2,071,249 + Psyr_1798 0.35 +0.4 2,071,249 + Psyr_1798 0.35 +1.0 2,071,250 - Psyr_1798 0.36 +0.2 2,071,250 - Psyr_1798 0.36 +3.7 2,071,250 - Psyr_1798 0.36 -3.3 2,071,250 - Psyr_1798 0.36 -4.6 2,071,250 - Psyr_1798 0.36 -1.6 2,071,250 - Psyr_1798 0.36 -2.3 2,071,251 + Psyr_1798 0.36 +3.2 2,071,354 + Psyr_1798 0.41 -1.2 2,071,355 - Psyr_1798 0.41 -0.7
Or see this region's nucleotide sequence