Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1753

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1751 and Psyr_1752 are separated by 104 nucleotidesPsyr_1752 and Psyr_1753 are separated by 80 nucleotidesPsyr_1753 and Psyr_1754 are separated by 205 nucleotides Psyr_1751: Psyr_1751 - PpiC-type peptidyl-prolyl cis-trans isomerase, at 1,990,043 to 1,991,926 _1751 Psyr_1752: Psyr_1752 - transcriptional regulator, HxlR family, at 1,992,031 to 1,992,369 _1752 Psyr_1753: Psyr_1753 - conserved hypothetical protein, at 1,992,450 to 1,992,776 _1753 Psyr_1754: Psyr_1754 - Enoyl-[acyl-carrier-protein] reductase [NADH], at 1,992,982 to 1,993,776 _1754 Position (kb) 1992 1993Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1991.722 kb on + strand, within Psyr_1751at 1991.883 kb on - strandat 1991.925 kb on - strandat 1991.996 kb on + strandat 1991.996 kb on + strandat 1991.997 kb on - strandat 1991.997 kb on - strandat 1991.997 kb on - strandat 1992.000 kb on + strandat 1992.000 kb on + strandat 1992.001 kb on - strandat 1992.001 kb on - strandat 1992.001 kb on - strandat 1992.001 kb on - strandat 1992.001 kb on - strandat 1992.014 kb on + strandat 1992.014 kb on + strandat 1992.015 kb on - strandat 1992.100 kb on - strand, within Psyr_1752at 1992.171 kb on + strand, within Psyr_1752at 1992.172 kb on - strand, within Psyr_1752at 1992.175 kb on + strand, within Psyr_1752at 1992.175 kb on + strand, within Psyr_1752at 1992.175 kb on + strand, within Psyr_1752at 1992.175 kb on + strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.176 kb on - strand, within Psyr_1752at 1992.305 kb on - strand, within Psyr_1752at 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.339 kb on + strandat 1992.340 kb on - strandat 1992.340 kb on - strandat 1992.340 kb on - strandat 1992.340 kb on - strandat 1992.340 kb on - strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.341 kb on + strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.342 kb on - strandat 1992.371 kb on + strandat 1992.372 kb on - strandat 1992.436 kb on - strandat 1992.455 kb on + strandat 1992.456 kb on - strandat 1992.456 kb on - strandat 1992.456 kb on - strandat 1992.483 kb on + strand, within Psyr_1753at 1992.621 kb on + strand, within Psyr_1753at 1992.621 kb on + strand, within Psyr_1753at 1992.622 kb on - strand, within Psyr_1753at 1992.622 kb on - strand, within Psyr_1753at 1992.622 kb on - strand, within Psyr_1753at 1992.627 kb on + strand, within Psyr_1753at 1992.627 kb on + strand, within Psyr_1753at 1992.627 kb on + strand, within Psyr_1753at 1992.627 kb on + strand, within Psyr_1753at 1992.628 kb on - strand, within Psyr_1753at 1992.629 kb on + strand, within Psyr_1753at 1992.629 kb on + strand, within Psyr_1753at 1992.629 kb on + strand, within Psyr_1753at 1992.630 kb on - strand, within Psyr_1753at 1992.630 kb on - strand, within Psyr_1753at 1992.682 kb on + strand, within Psyr_1753at 1992.682 kb on + strand, within Psyr_1753at 1992.682 kb on + strand, within Psyr_1753at 1992.683 kb on - strand, within Psyr_1753at 1992.683 kb on - strand, within Psyr_1753at 1992.683 kb on - strand, within Psyr_1753at 1992.690 kb on - strand, within Psyr_1753at 1992.830 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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1,991,722 + Psyr_1751 0.89 -2.0
1,991,883 - +2.5
1,991,925 - +3.3
1,991,996 + -1.8
1,991,996 + +4.4
1,991,997 - +0.3
1,991,997 - +3.7
1,991,997 - +0.2
1,992,000 + -0.0
1,992,000 + -0.5
1,992,001 - +0.7
1,992,001 - -2.1
1,992,001 - -4.7
1,992,001 - -0.8
1,992,001 - +4.0
1,992,014 + +2.3
1,992,014 + -1.8
1,992,015 - -2.2
1,992,100 - Psyr_1752 0.20 +1.0
1,992,171 + Psyr_1752 0.41 +1.2
1,992,172 - Psyr_1752 0.42 -0.0
1,992,175 + Psyr_1752 0.42 +3.8
1,992,175 + Psyr_1752 0.42 -1.2
1,992,175 + Psyr_1752 0.42 -1.0
1,992,175 + Psyr_1752 0.42 -3.1
1,992,176 - Psyr_1752 0.43 -2.2
1,992,176 - Psyr_1752 0.43 +3.0
1,992,176 - Psyr_1752 0.43 -0.2
1,992,176 - Psyr_1752 0.43 +3.3
1,992,176 - Psyr_1752 0.43 +3.7
1,992,176 - Psyr_1752 0.43 -1.3
1,992,305 - Psyr_1752 0.81 -1.0
1,992,339 + +3.1
1,992,339 + +0.2
1,992,339 + -1.5
1,992,339 + +0.5
1,992,339 + -2.3
1,992,339 + +3.4
1,992,339 + -1.2
1,992,340 - -1.0
1,992,340 - +2.8
1,992,340 - -0.8
1,992,340 - +2.8
1,992,340 - +2.5
1,992,341 + -0.5
1,992,341 + +3.0
1,992,341 + +1.5
1,992,341 + +1.5
1,992,341 + -0.5
1,992,341 + +3.3
1,992,341 + -1.9
1,992,341 + +2.8
1,992,342 - +3.4
1,992,342 - +0.3
1,992,342 - +2.3
1,992,342 - +2.4
1,992,342 - -2.1
1,992,342 - +3.9
1,992,342 - -1.3
1,992,342 - -1.4
1,992,342 - +4.1
1,992,342 - -1.4
1,992,342 - +0.2
1,992,371 + -0.3
1,992,372 - +4.3
1,992,436 - -0.4
1,992,455 + +2.0
1,992,456 - +3.7
1,992,456 - +3.8
1,992,456 - +3.9
1,992,483 + Psyr_1753 0.10 -0.6
1,992,621 + Psyr_1753 0.52 +1.0
1,992,621 + Psyr_1753 0.52 -0.0
1,992,622 - Psyr_1753 0.53 +3.1
1,992,622 - Psyr_1753 0.53 -1.8
1,992,622 - Psyr_1753 0.53 -0.2
1,992,627 + Psyr_1753 0.54 +3.1
1,992,627 + Psyr_1753 0.54 -0.7
1,992,627 + Psyr_1753 0.54 +0.9
1,992,627 + Psyr_1753 0.54 -0.1
1,992,628 - Psyr_1753 0.54 +1.5
1,992,629 + Psyr_1753 0.55 +1.0
1,992,629 + Psyr_1753 0.55 -0.5
1,992,629 + Psyr_1753 0.55 -0.7
1,992,630 - Psyr_1753 0.55 +2.8
1,992,630 - Psyr_1753 0.55 -0.3
1,992,682 + Psyr_1753 0.71 -0.4
1,992,682 + Psyr_1753 0.71 +1.7
1,992,682 + Psyr_1753 0.71 -0.6
1,992,683 - Psyr_1753 0.71 +2.5
1,992,683 - Psyr_1753 0.71 -1.4
1,992,683 - Psyr_1753 0.71 +3.3
1,992,690 - Psyr_1753 0.73 -0.5
1,992,830 + +3.8

Or see this region's nucleotide sequence