Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1749 and Psyr_1750 are separated by 146 nucleotides Psyr_1750 and Psyr_1751 are separated by 205 nucleotides Psyr_1751 and Psyr_1752 are separated by 104 nucleotides Psyr_1752 and Psyr_1753 are separated by 80 nucleotides
Psyr_1749: Psyr_1749 - ATP-dependent proteinase, Serine peptidase, MEROPS family S16, at 1,987,022 to 1,989,418
_1749
Psyr_1750: Psyr_1750 - bacterial nucleoid protein HU beta subunit, at 1,989,565 to 1,989,837
_1750
Psyr_1751: Psyr_1751 - PpiC-type peptidyl-prolyl cis-trans isomerase, at 1,990,043 to 1,991,926
_1751
Psyr_1752: Psyr_1752 - transcriptional regulator, HxlR family, at 1,992,031 to 1,992,369
_1752
Psyr_1753: Psyr_1753 - conserved hypothetical protein, at 1,992,450 to 1,992,776
_1753
Position (kb)
1990
1991
1992 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1989.256 kb on + strand at 1989.352 kb on + strand at 1989.406 kb on + strand at 1989.406 kb on + strand at 1989.416 kb on + strand at 1989.416 kb on + strand at 1989.459 kb on + strand at 1989.461 kb on + strand at 1989.461 kb on + strand at 1989.551 kb on + strand at 1989.561 kb on + strand at 1989.709 kb on - strand, within Psyr_1750 at 1989.835 kb on + strand at 1989.982 kb on + strand at 1989.982 kb on + strand at 1990.021 kb on + strand at 1990.021 kb on + strand at 1990.021 kb on + strand at 1990.021 kb on + strand at 1990.022 kb on - strand at 1990.055 kb on - strand at 1990.482 kb on + strand, within Psyr_1751 at 1990.483 kb on - strand, within Psyr_1751 at 1990.483 kb on - strand, within Psyr_1751 at 1990.487 kb on + strand, within Psyr_1751 at 1990.695 kb on - strand, within Psyr_1751 at 1990.745 kb on + strand, within Psyr_1751 at 1991.036 kb on + strand, within Psyr_1751 at 1991.037 kb on - strand, within Psyr_1751 at 1991.037 kb on - strand, within Psyr_1751 at 1991.037 kb on - strand, within Psyr_1751 at 1991.063 kb on + strand, within Psyr_1751 at 1991.063 kb on + strand, within Psyr_1751 at 1991.064 kb on - strand, within Psyr_1751 at 1991.064 kb on - strand, within Psyr_1751 at 1991.064 kb on - strand, within Psyr_1751 at 1991.064 kb on - strand, within Psyr_1751 at 1991.419 kb on + strand, within Psyr_1751 at 1991.722 kb on + strand, within Psyr_1751 at 1991.883 kb on - strand at 1991.925 kb on - strand at 1991.996 kb on + strand at 1991.996 kb on + strand at 1991.997 kb on - strand at 1991.997 kb on - strand at 1991.997 kb on - strand at 1992.000 kb on + strand at 1992.000 kb on + strand at 1992.001 kb on - strand at 1992.001 kb on - strand at 1992.001 kb on - strand at 1992.001 kb on - strand at 1992.001 kb on - strand at 1992.014 kb on + strand at 1992.014 kb on + strand at 1992.015 kb on - strand at 1992.100 kb on - strand, within Psyr_1752 at 1992.171 kb on + strand, within Psyr_1752 at 1992.172 kb on - strand, within Psyr_1752 at 1992.175 kb on + strand, within Psyr_1752 at 1992.175 kb on + strand, within Psyr_1752 at 1992.175 kb on + strand, within Psyr_1752 at 1992.175 kb on + strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.176 kb on - strand, within Psyr_1752 at 1992.305 kb on - strand, within Psyr_1752 at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.341 kb on + strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.342 kb on - strand at 1992.371 kb on + strand at 1992.372 kb on - strand at 1992.436 kb on - strand at 1992.455 kb on + strand at 1992.456 kb on - strand at 1992.456 kb on - strand at 1992.456 kb on - strand at 1992.483 kb on + strand, within Psyr_1753 at 1992.621 kb on + strand, within Psyr_1753 at 1992.621 kb on + strand, within Psyr_1753 at 1992.622 kb on - strand, within Psyr_1753 at 1992.622 kb on - strand, within Psyr_1753 at 1992.622 kb on - strand, within Psyr_1753 at 1992.627 kb on + strand, within Psyr_1753 at 1992.627 kb on + strand, within Psyr_1753 at 1992.627 kb on + strand, within Psyr_1753 at 1992.627 kb on + strand, within Psyr_1753 at 1992.628 kb on - strand, within Psyr_1753 at 1992.629 kb on + strand, within Psyr_1753 at 1992.629 kb on + strand, within Psyr_1753 at 1992.629 kb on + strand, within Psyr_1753 at 1992.630 kb on - strand, within Psyr_1753 at 1992.630 kb on - strand, within Psyr_1753 at 1992.682 kb on + strand, within Psyr_1753 at 1992.682 kb on + strand, within Psyr_1753 at 1992.682 kb on + strand, within Psyr_1753 at 1992.683 kb on - strand, within Psyr_1753 at 1992.683 kb on - strand, within Psyr_1753 at 1992.683 kb on - strand, within Psyr_1753 at 1992.690 kb on - strand, within Psyr_1753 at 1992.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,989,256 + -1.2 1,989,352 + -1.6 1,989,406 + -0.6 1,989,406 + +1.5 1,989,416 + -0.7 1,989,416 + -2.4 1,989,459 + +3.3 1,989,461 + -4.2 1,989,461 + +3.9 1,989,551 + +1.5 1,989,561 + +1.5 1,989,709 - Psyr_1750 0.53 +1.5 1,989,835 + +1.0 1,989,982 + +0.4 1,989,982 + -1.4 1,990,021 + -0.8 1,990,021 + -2.0 1,990,021 + +1.4 1,990,021 + +3.3 1,990,022 - -2.6 1,990,055 - -2.9 1,990,482 + Psyr_1751 0.23 +2.3 1,990,483 - Psyr_1751 0.23 -2.1 1,990,483 - Psyr_1751 0.23 -3.4 1,990,487 + Psyr_1751 0.24 -0.9 1,990,695 - Psyr_1751 0.35 -3.5 1,990,745 + Psyr_1751 0.37 -2.2 1,991,036 + Psyr_1751 0.53 +1.5 1,991,037 - Psyr_1751 0.53 -0.0 1,991,037 - Psyr_1751 0.53 -0.0 1,991,037 - Psyr_1751 0.53 +1.0 1,991,063 + Psyr_1751 0.54 +1.0 1,991,063 + Psyr_1751 0.54 -2.9 1,991,064 - Psyr_1751 0.54 -0.0 1,991,064 - Psyr_1751 0.54 -3.6 1,991,064 - Psyr_1751 0.54 -0.0 1,991,064 - Psyr_1751 0.54 -4.1 1,991,419 + Psyr_1751 0.73 +1.5 1,991,722 + Psyr_1751 0.89 -2.0 1,991,883 - +2.5 1,991,925 - +3.3 1,991,996 + -1.8 1,991,996 + +4.4 1,991,997 - +0.3 1,991,997 - +3.7 1,991,997 - +0.2 1,992,000 + -0.0 1,992,000 + -0.5 1,992,001 - +0.7 1,992,001 - -2.1 1,992,001 - -4.7 1,992,001 - -0.8 1,992,001 - +4.0 1,992,014 + +2.3 1,992,014 + -1.8 1,992,015 - -2.2 1,992,100 - Psyr_1752 0.20 +1.0 1,992,171 + Psyr_1752 0.41 +1.2 1,992,172 - Psyr_1752 0.42 -0.0 1,992,175 + Psyr_1752 0.42 +3.8 1,992,175 + Psyr_1752 0.42 -1.2 1,992,175 + Psyr_1752 0.42 -1.0 1,992,175 + Psyr_1752 0.42 -3.1 1,992,176 - Psyr_1752 0.43 -2.2 1,992,176 - Psyr_1752 0.43 +3.0 1,992,176 - Psyr_1752 0.43 -0.2 1,992,176 - Psyr_1752 0.43 +3.3 1,992,176 - Psyr_1752 0.43 +3.7 1,992,176 - Psyr_1752 0.43 -1.3 1,992,305 - Psyr_1752 0.81 -1.0 1,992,339 + +3.1 1,992,339 + +0.2 1,992,339 + -1.5 1,992,339 + +0.5 1,992,339 + -2.3 1,992,339 + +3.4 1,992,339 + -1.2 1,992,340 - -1.0 1,992,340 - +2.8 1,992,340 - -0.8 1,992,340 - +2.8 1,992,340 - +2.5 1,992,341 + -0.5 1,992,341 + +3.0 1,992,341 + +1.5 1,992,341 + +1.5 1,992,341 + -0.5 1,992,341 + +3.3 1,992,341 + -1.9 1,992,341 + +2.8 1,992,342 - +3.4 1,992,342 - +0.3 1,992,342 - +2.3 1,992,342 - +2.4 1,992,342 - -2.1 1,992,342 - +3.9 1,992,342 - -1.3 1,992,342 - -1.4 1,992,342 - +4.1 1,992,342 - -1.4 1,992,342 - +0.2 1,992,371 + -0.3 1,992,372 - +4.3 1,992,436 - -0.4 1,992,455 + +2.0 1,992,456 - +3.7 1,992,456 - +3.8 1,992,456 - +3.9 1,992,483 + Psyr_1753 0.10 -0.6 1,992,621 + Psyr_1753 0.52 +1.0 1,992,621 + Psyr_1753 0.52 -0.0 1,992,622 - Psyr_1753 0.53 +3.1 1,992,622 - Psyr_1753 0.53 -1.8 1,992,622 - Psyr_1753 0.53 -0.2 1,992,627 + Psyr_1753 0.54 +3.1 1,992,627 + Psyr_1753 0.54 -0.7 1,992,627 + Psyr_1753 0.54 +0.9 1,992,627 + Psyr_1753 0.54 -0.1 1,992,628 - Psyr_1753 0.54 +1.5 1,992,629 + Psyr_1753 0.55 +1.0 1,992,629 + Psyr_1753 0.55 -0.5 1,992,629 + Psyr_1753 0.55 -0.7 1,992,630 - Psyr_1753 0.55 +2.8 1,992,630 - Psyr_1753 0.55 -0.3 1,992,682 + Psyr_1753 0.71 -0.4 1,992,682 + Psyr_1753 0.71 +1.7 1,992,682 + Psyr_1753 0.71 -0.6 1,992,683 - Psyr_1753 0.71 +2.5 1,992,683 - Psyr_1753 0.71 -1.4 1,992,683 - Psyr_1753 0.71 +3.3 1,992,690 - Psyr_1753 0.73 -0.5 1,992,830 + +3.8
Or see this region's nucleotide sequence