Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1746 and Psyr_1747 are separated by 91 nucleotides Psyr_1747 and Psyr_1748 are separated by 106 nucleotides
Psyr_1746: Psyr_1746 - Trigger factor, at 1,983,381 to 1,984,691
_1746
Psyr_1747: Psyr_1747 - ATP-dependent Clp protease proteolytic subunit ClpP, at 1,984,783 to 1,985,424
_1747
Psyr_1748: Psyr_1748 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,985,531 to 1,986,814
_1748
Position (kb)
1984
1985
1986 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1984.041 kb on + strand, within Psyr_1746 at 1984.532 kb on - strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.571 kb on - strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.647 kb on + strand at 1984.761 kb on + strand at 1984.762 kb on - strand at 1984.799 kb on - strand at 1984.882 kb on + strand, within Psyr_1747 at 1984.891 kb on + strand, within Psyr_1747 at 1984.892 kb on - strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.423 kb on - strand at 1985.423 kb on - strand at 1985.573 kb on + strand at 1985.573 kb on + strand at 1985.574 kb on - strand at 1985.638 kb on + strand at 1985.719 kb on + strand, within Psyr_1748 at 1985.719 kb on + strand, within Psyr_1748 at 1985.720 kb on - strand, within Psyr_1748 at 1985.766 kb on + strand, within Psyr_1748 at 1985.767 kb on - strand, within Psyr_1748 at 1985.810 kb on + strand, within Psyr_1748 at 1985.810 kb on + strand, within Psyr_1748 at 1985.811 kb on - strand, within Psyr_1748 at 1985.819 kb on + strand, within Psyr_1748 at 1985.949 kb on + strand, within Psyr_1748 at 1985.993 kb on + strand, within Psyr_1748 at 1985.994 kb on - strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.080 kb on + strand, within Psyr_1748 at 1986.080 kb on + strand, within Psyr_1748 at 1986.259 kb on + strand, within Psyr_1748 at 1986.260 kb on - strand, within Psyr_1748 at 1986.272 kb on - strand, within Psyr_1748
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,984,041 + Psyr_1746 0.50 -1.1 1,984,532 - Psyr_1746 0.88 -1.6 1,984,542 + Psyr_1746 0.89 +0.5 1,984,542 + Psyr_1746 0.89 -2.0 1,984,542 + Psyr_1746 0.89 +3.5 1,984,542 + Psyr_1746 0.89 -0.8 1,984,570 + -3.0 1,984,570 + +1.5 1,984,570 + -1.4 1,984,570 + +3.4 1,984,571 - +1.2 1,984,600 + +4.2 1,984,600 + -2.1 1,984,600 + -0.4 1,984,600 + +1.5 1,984,600 + +2.8 1,984,604 + +2.5 1,984,604 + -1.4 1,984,604 + +0.2 1,984,604 + +2.8 1,984,647 + -2.2 1,984,761 + -0.0 1,984,762 - +0.3 1,984,799 - -3.6 1,984,882 + Psyr_1747 0.15 -0.0 1,984,891 + Psyr_1747 0.17 -4.2 1,984,892 - Psyr_1747 0.17 -2.7 1,984,945 + Psyr_1747 0.25 -2.9 1,984,946 - Psyr_1747 0.25 +1.5 1,984,946 - Psyr_1747 0.25 +2.0 1,985,017 + Psyr_1747 0.36 -5.4 1,985,017 + Psyr_1747 0.36 -0.0 1,985,017 + Psyr_1747 0.36 +1.5 1,985,018 - Psyr_1747 0.37 +1.5 1,985,018 - Psyr_1747 0.37 +2.0 1,985,018 - Psyr_1747 0.37 -2.6 1,985,018 - Psyr_1747 0.37 -3.3 1,985,018 - Psyr_1747 0.37 +1.5 1,985,423 - -0.0 1,985,423 - -0.0 1,985,573 + -4.3 1,985,573 + -3.6 1,985,574 - +1.0 1,985,638 + +0.9 1,985,719 + Psyr_1748 0.15 -1.4 1,985,719 + Psyr_1748 0.15 -2.4 1,985,720 - Psyr_1748 0.15 +0.4 1,985,766 + Psyr_1748 0.18 +0.9 1,985,767 - Psyr_1748 0.18 -2.6 1,985,810 + Psyr_1748 0.22 +2.5 1,985,810 + Psyr_1748 0.22 -3.4 1,985,811 - Psyr_1748 0.22 -4.0 1,985,819 + Psyr_1748 0.22 +2.8 1,985,949 + Psyr_1748 0.33 +3.5 1,985,993 + Psyr_1748 0.36 -0.1 1,985,994 - Psyr_1748 0.36 -0.1 1,986,054 + Psyr_1748 0.41 -3.6 1,986,054 + Psyr_1748 0.41 +0.9 1,986,054 + Psyr_1748 0.41 +0.9 1,986,055 - Psyr_1748 0.41 -0.1 1,986,055 - Psyr_1748 0.41 -1.8 1,986,055 - Psyr_1748 0.41 -4.8 1,986,080 + Psyr_1748 0.43 +1.5 1,986,080 + Psyr_1748 0.43 +1.5 1,986,259 + Psyr_1748 0.57 -2.5 1,986,260 - Psyr_1748 0.57 +0.9 1,986,272 - Psyr_1748 0.58 -5.1
Or see this region's nucleotide sequence