Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1724 and Psyr_1725 are separated by 86 nucleotides Psyr_1725 and Psyr_1726 are separated by 314 nucleotides
Psyr_1724: Psyr_1724 - glutamate dehydrogenase (NAD), at 1,953,126 to 1,957,982
_1724
Psyr_1725: Psyr_1725 - Protein of unknown function DUF204, at 1,958,069 to 1,958,605
_1725
Psyr_1726: Psyr_1726 - aconitase, at 1,958,920 to 1,961,535
_1726
Position (kb)
1958
1959 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1957.293 kb on + strand, within Psyr_1724 at 1957.294 kb on - strand, within Psyr_1724 at 1957.509 kb on + strand at 1957.510 kb on - strand at 1957.510 kb on - strand at 1957.510 kb on - strand at 1957.557 kb on - strand at 1957.569 kb on + strand at 1957.569 kb on + strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.571 kb on + strand at 1957.571 kb on + strand at 1957.572 kb on - strand at 1957.572 kb on - strand at 1957.638 kb on + strand at 1957.638 kb on + strand at 1957.638 kb on + strand at 1957.667 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.981 kb on - strand at 1957.992 kb on - strand at 1957.992 kb on - strand at 1958.006 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.246 kb on - strand, within Psyr_1725 at 1958.270 kb on + strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.554 kb on + strand at 1958.554 kb on + strand at 1958.555 kb on - strand at 1958.645 kb on + strand at 1958.646 kb on - strand at 1958.647 kb on + strand at 1958.822 kb on + strand at 1958.822 kb on + strand at 1958.823 kb on - strand at 1958.823 kb on - strand at 1958.824 kb on + strand at 1958.825 kb on - strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.847 kb on + strand at 1958.847 kb on + strand at 1958.847 kb on + strand at 1958.848 kb on - strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.917 kb on + strand at 1958.918 kb on - strand at 1958.921 kb on + strand at 1958.921 kb on + strand at 1958.922 kb on - strand at 1958.922 kb on - strand at 1959.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,957,293 + Psyr_1724 0.86 +0.7 1,957,294 - Psyr_1724 0.86 -2.1 1,957,509 + -1.1 1,957,510 - -1.0 1,957,510 - +2.8 1,957,510 - +1.0 1,957,557 - +2.0 1,957,569 + -0.9 1,957,569 + -0.7 1,957,570 - +2.5 1,957,570 - +3.1 1,957,570 - -0.1 1,957,570 - +1.0 1,957,570 - +3.1 1,957,571 + +3.0 1,957,571 + +3.1 1,957,572 - -0.0 1,957,572 - +2.0 1,957,638 + +1.0 1,957,638 + -1.6 1,957,638 + -0.2 1,957,667 + -1.9 1,957,920 + -1.4 1,957,920 + +2.3 1,957,920 + -0.7 1,957,920 + +3.0 1,957,920 + -2.4 1,957,920 + +0.4 1,957,920 + +2.0 1,957,920 + +3.7 1,957,921 - +0.2 1,957,921 - +1.5 1,957,921 - -1.9 1,957,921 - -3.9 1,957,921 - +2.4 1,957,921 - -0.0 1,957,921 - +1.5 1,957,981 - -4.1 1,957,992 - +1.4 1,957,992 - +1.0 1,958,006 + +3.8 1,958,052 + +1.0 1,958,052 + +0.6 1,958,052 + +3.3 1,958,052 + +1.5 1,958,246 - Psyr_1725 0.33 -1.0 1,958,270 + Psyr_1725 0.37 +3.3 1,958,386 - Psyr_1725 0.59 +3.8 1,958,517 - Psyr_1725 0.83 -2.0 1,958,517 - Psyr_1725 0.83 +1.5 1,958,517 - Psyr_1725 0.83 +4.0 1,958,554 + +2.5 1,958,554 + -0.3 1,958,555 - +1.3 1,958,645 + -1.4 1,958,646 - +1.0 1,958,647 + +3.8 1,958,822 + +3.0 1,958,822 + -2.6 1,958,823 - -2.2 1,958,823 - +1.0 1,958,824 + +3.0 1,958,825 - -1.6 1,958,845 + +4.2 1,958,845 + -2.6 1,958,845 + -0.8 1,958,845 + -0.7 1,958,845 + +2.0 1,958,846 - -1.5 1,958,846 - -2.4 1,958,846 - -1.2 1,958,846 - -0.9 1,958,847 + +4.0 1,958,847 + +3.7 1,958,847 + -0.0 1,958,848 - -0.0 1,958,851 + -1.0 1,958,851 + +1.0 1,958,851 + +3.3 1,958,852 - -1.6 1,958,852 - -0.3 1,958,852 - +1.4 1,958,852 - +3.1 1,958,917 + +3.1 1,958,918 - -3.2 1,958,921 + -0.0 1,958,921 + -0.0 1,958,922 - -1.5 1,958,922 - +1.5 1,959,000 - -0.0
Or see this region's nucleotide sequence