Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1719 and Psyr_1720 overlap by 4 nucleotides Psyr_1720 and Psyr_1721 are separated by 4 nucleotides Psyr_1721 and Psyr_1722 are separated by 209 nucleotides
Psyr_1719: Psyr_1719 - conserved hypothetical protein, at 1,948,996 to 1,949,490
_1719
Psyr_1720: Psyr_1720 - Protein of unknown function DUF58, at 1,949,487 to 1,950,431
_1720
Psyr_1721: Psyr_1721 - moxR protein, putative, at 1,950,436 to 1,951,395
_1721
Psyr_1722: Psyr_1722 - Amidohydrolase 2, at 1,951,605 to 1,952,351
_1722
Position (kb) 1950
1951
1952 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 1949.521 kb on + strand at 1949.996 kb on - strand, within Psyr_1720 at 1949.996 kb on - strand, within Psyr_1720 at 1950.200 kb on + strand, within Psyr_1720 at 1950.201 kb on - strand, within Psyr_1720 at 1950.250 kb on + strand, within Psyr_1720 at 1950.251 kb on - strand, within Psyr_1720 at 1950.722 kb on + strand, within Psyr_1721 at 1950.722 kb on + strand, within Psyr_1721 at 1950.723 kb on - strand, within Psyr_1721 at 1950.723 kb on - strand, within Psyr_1721 at 1950.746 kb on + strand, within Psyr_1721 at 1950.746 kb on + strand, within Psyr_1721 at 1950.746 kb on + strand, within Psyr_1721 at 1950.747 kb on - strand, within Psyr_1721 at 1950.747 kb on - strand, within Psyr_1721 at 1950.747 kb on - strand, within Psyr_1721 at 1950.995 kb on + strand, within Psyr_1721 at 1950.995 kb on + strand, within Psyr_1721 at 1950.995 kb on + strand, within Psyr_1721 at 1950.996 kb on - strand, within Psyr_1721 at 1950.996 kb on - strand, within Psyr_1721 at 1951.455 kb on + strand at 1951.455 kb on + strand at 1951.531 kb on + strand at 1951.532 kb on - strand at 1951.532 kb on - strand at 1951.532 kb on - strand at 1951.532 kb on - strand at 1951.539 kb on + strand at 1951.539 kb on + strand at 1951.547 kb on + strand at 1951.548 kb on - strand at 1951.570 kb on + strand at 1951.570 kb on + strand at 1951.571 kb on - strand at 1951.571 kb on - strand at 1951.571 kb on - strand at 1951.571 kb on - strand at 1951.571 kb on - strand at 1951.833 kb on + strand, within Psyr_1722 at 1951.960 kb on + strand, within Psyr_1722 at 1951.960 kb on + strand, within Psyr_1722 at 1951.960 kb on + strand, within Psyr_1722 at 1951.961 kb on - strand, within Psyr_1722 at 1951.961 kb on - strand, within Psyr_1722 at 1951.961 kb on - strand, within Psyr_1722 at 1952.174 kb on - strand, within Psyr_1722 at 1952.251 kb on + strand, within Psyr_1722
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,949,521 + -1.6 1,949,996 - Psyr_1720 0.54 -1.4 1,949,996 - Psyr_1720 0.54 +1.0 1,950,200 + Psyr_1720 0.75 +1.5 1,950,201 - Psyr_1720 0.76 -1.1 1,950,250 + Psyr_1720 0.81 +2.8 1,950,251 - Psyr_1720 0.81 -0.5 1,950,722 + Psyr_1721 0.30 -4.2 1,950,722 + Psyr_1721 0.30 -0.0 1,950,723 - Psyr_1721 0.30 -1.2 1,950,723 - Psyr_1721 0.30 -1.9 1,950,746 + Psyr_1721 0.32 +3.3 1,950,746 + Psyr_1721 0.32 +1.5 1,950,746 + Psyr_1721 0.32 +3.1 1,950,747 - Psyr_1721 0.32 -3.0 1,950,747 - Psyr_1721 0.32 +1.5 1,950,747 - Psyr_1721 0.32 -0.9 1,950,995 + Psyr_1721 0.58 -3.1 1,950,995 + Psyr_1721 0.58 -0.9 1,950,995 + Psyr_1721 0.58 -3.2 1,950,996 - Psyr_1721 0.58 -2.0 1,950,996 - Psyr_1721 0.58 -0.4 1,951,455 + +1.0 1,951,455 + -0.6 1,951,531 + -0.4 1,951,532 - +2.5 1,951,532 - -1.8 1,951,532 - +0.3 1,951,532 - +2.0 1,951,539 + +0.9 1,951,539 + -0.0 1,951,547 + +3.4 1,951,548 - +2.8 1,951,570 + -1.0 1,951,570 + +2.5 1,951,571 - +2.5 1,951,571 - -2.2 1,951,571 - +3.0 1,951,571 - +2.8 1,951,571 - +2.0 1,951,833 + Psyr_1722 0.31 +1.1 1,951,960 + Psyr_1722 0.48 -3.1 1,951,960 + Psyr_1722 0.48 -2.2 1,951,960 + Psyr_1722 0.48 +4.5 1,951,961 - Psyr_1722 0.48 -2.5 1,951,961 - Psyr_1722 0.48 +2.3 1,951,961 - Psyr_1722 0.48 +4.0 1,952,174 - Psyr_1722 0.76 -0.5 1,952,251 + Psyr_1722 0.86 -1.4
Or see this region's nucleotide sequence