Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1709 and Psyr_1710 are separated by 35 nucleotides Psyr_1710 and Psyr_1711 are separated by 126 nucleotides Psyr_1711 and Psyr_1712 are separated by 66 nucleotides
Psyr_1709: Psyr_1709 - Major facilitator superfamily, at 1,934,834 to 1,936,147
_1709
Psyr_1710: Psyr_1710 - Protein of unknown function DUF1238, at 1,936,183 to 1,937,175
_1710
Psyr_1711: Psyr_1711 - conserved hypothetical protein, at 1,937,302 to 1,937,598
_1711
Psyr_1712: Psyr_1712 - conserved hypothetical protein, at 1,937,665 to 1,938,849
_1712
Position (kb)
1936
1937
1938 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1935.206 kb on + strand, within Psyr_1709 at 1935.206 kb on + strand, within Psyr_1709 at 1935.206 kb on + strand, within Psyr_1709 at 1935.206 kb on + strand, within Psyr_1709 at 1935.207 kb on - strand, within Psyr_1709 at 1935.207 kb on - strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.209 kb on + strand, within Psyr_1709 at 1935.210 kb on - strand, within Psyr_1709 at 1935.210 kb on - strand at 1935.210 kb on - strand, within Psyr_1709 at 1935.210 kb on - strand, within Psyr_1709 at 1935.210 kb on - strand, within Psyr_1709 at 1935.504 kb on - strand, within Psyr_1709 at 1935.576 kb on - strand, within Psyr_1709 at 1935.576 kb on - strand, within Psyr_1709 at 1935.599 kb on + strand, within Psyr_1709 at 1935.599 kb on + strand, within Psyr_1709 at 1935.600 kb on - strand, within Psyr_1709 at 1935.600 kb on - strand, within Psyr_1709 at 1935.683 kb on + strand, within Psyr_1709 at 1935.767 kb on + strand, within Psyr_1709 at 1935.767 kb on + strand, within Psyr_1709 at 1935.767 kb on + strand, within Psyr_1709 at 1935.767 kb on + strand, within Psyr_1709 at 1935.767 kb on + strand, within Psyr_1709 at 1935.768 kb on - strand, within Psyr_1709 at 1935.768 kb on - strand, within Psyr_1709 at 1935.768 kb on - strand, within Psyr_1709 at 1935.768 kb on - strand, within Psyr_1709 at 1935.965 kb on + strand, within Psyr_1709 at 1936.028 kb on + strand at 1936.145 kb on + strand at 1936.146 kb on - strand at 1936.193 kb on + strand at 1936.193 kb on + strand at 1936.194 kb on - strand at 1936.530 kb on + strand, within Psyr_1710 at 1936.531 kb on - strand, within Psyr_1710 at 1936.600 kb on + strand, within Psyr_1710 at 1936.699 kb on + strand, within Psyr_1710 at 1936.699 kb on + strand, within Psyr_1710 at 1936.699 kb on + strand, within Psyr_1710 at 1936.700 kb on - strand, within Psyr_1710 at 1936.700 kb on - strand, within Psyr_1710 at 1936.700 kb on - strand, within Psyr_1710 at 1936.700 kb on - strand, within Psyr_1710 at 1936.700 kb on - strand, within Psyr_1710 at 1936.757 kb on - strand, within Psyr_1710 at 1936.757 kb on - strand, within Psyr_1710 at 1936.786 kb on + strand, within Psyr_1710 at 1936.787 kb on - strand, within Psyr_1710 at 1936.787 kb on - strand, within Psyr_1710 at 1936.787 kb on - strand, within Psyr_1710 at 1936.787 kb on - strand at 1936.787 kb on - strand, within Psyr_1710 at 1936.792 kb on + strand, within Psyr_1710 at 1936.793 kb on - strand, within Psyr_1710 at 1936.793 kb on - strand, within Psyr_1710 at 1936.793 kb on - strand, within Psyr_1710 at 1936.793 kb on - strand, within Psyr_1710 at 1936.793 kb on - strand, within Psyr_1710 at 1937.173 kb on + strand at 1937.174 kb on - strand at 1937.231 kb on - strand at 1937.239 kb on - strand at 1937.255 kb on + strand at 1937.255 kb on + strand at 1937.256 kb on - strand at 1937.256 kb on - strand at 1937.316 kb on + strand at 1937.316 kb on + strand at 1937.316 kb on + strand at 1937.448 kb on - strand, within Psyr_1711 at 1937.600 kb on + strand at 1937.600 kb on + strand at 1937.601 kb on - strand at 1937.601 kb on - strand at 1937.622 kb on + strand at 1937.622 kb on + strand at 1937.622 kb on + strand at 1937.668 kb on + strand at 1937.686 kb on - strand at 1937.757 kb on + strand at 1937.758 kb on - strand at 1937.806 kb on + strand, within Psyr_1712 at 1937.807 kb on - strand, within Psyr_1712 at 1937.807 kb on - strand, within Psyr_1712 at 1937.807 kb on - strand, within Psyr_1712 at 1937.807 kb on - strand, within Psyr_1712 at 1938.062 kb on - strand, within Psyr_1712 at 1938.092 kb on - strand, within Psyr_1712 at 1938.098 kb on + strand, within Psyr_1712
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,935,206 + Psyr_1709 0.28 +1.6 1,935,206 + Psyr_1709 0.28 -4.7 1,935,206 + Psyr_1709 0.28 +1.0 1,935,206 + Psyr_1709 0.28 -0.8 1,935,207 - Psyr_1709 0.28 -0.7 1,935,207 - Psyr_1709 0.28 +1.6 1,935,209 + Psyr_1709 0.29 -0.9 1,935,209 + Psyr_1709 0.29 +1.6 1,935,209 + Psyr_1709 0.29 +2.0 1,935,209 + Psyr_1709 0.29 +2.0 1,935,209 + Psyr_1709 0.29 +0.4 1,935,209 + Psyr_1709 0.29 -0.5 1,935,209 + Psyr_1709 0.29 -0.4 1,935,209 + Psyr_1709 0.29 -0.0 1,935,210 - Psyr_1709 0.29 -1.5 1,935,210 - -0.0 1,935,210 - Psyr_1709 0.29 +2.0 1,935,210 - Psyr_1709 0.29 +2.0 1,935,210 - Psyr_1709 0.29 -0.0 1,935,504 - Psyr_1709 0.51 +2.0 1,935,576 - Psyr_1709 0.56 +3.8 1,935,576 - Psyr_1709 0.56 +2.6 1,935,599 + Psyr_1709 0.58 +2.3 1,935,599 + Psyr_1709 0.58 +1.1 1,935,600 - Psyr_1709 0.58 -0.8 1,935,600 - Psyr_1709 0.58 -0.3 1,935,683 + Psyr_1709 0.65 -1.1 1,935,767 + Psyr_1709 0.71 -1.2 1,935,767 + Psyr_1709 0.71 -0.8 1,935,767 + Psyr_1709 0.71 -1.2 1,935,767 + Psyr_1709 0.71 +4.6 1,935,767 + Psyr_1709 0.71 +0.8 1,935,768 - Psyr_1709 0.71 +1.6 1,935,768 - Psyr_1709 0.71 +4.6 1,935,768 - Psyr_1709 0.71 -1.6 1,935,768 - Psyr_1709 0.71 -0.8 1,935,965 + Psyr_1709 0.86 -0.0 1,936,028 + -1.4 1,936,145 + +0.6 1,936,146 - +0.1 1,936,193 + -0.2 1,936,193 + -0.3 1,936,194 - +1.6 1,936,530 + Psyr_1710 0.35 -3.2 1,936,531 - Psyr_1710 0.35 +0.2 1,936,600 + Psyr_1710 0.42 +3.1 1,936,699 + Psyr_1710 0.52 +2.8 1,936,699 + Psyr_1710 0.52 -0.6 1,936,699 + Psyr_1710 0.52 -1.2 1,936,700 - Psyr_1710 0.52 +0.3 1,936,700 - Psyr_1710 0.52 -0.0 1,936,700 - Psyr_1710 0.52 +3.1 1,936,700 - Psyr_1710 0.52 -0.9 1,936,700 - Psyr_1710 0.52 +2.0 1,936,757 - Psyr_1710 0.58 +2.0 1,936,757 - Psyr_1710 0.58 +3.1 1,936,786 + Psyr_1710 0.61 +4.3 1,936,787 - Psyr_1710 0.61 +3.1 1,936,787 - Psyr_1710 0.61 +1.6 1,936,787 - Psyr_1710 0.61 -0.8 1,936,787 - +2.3 1,936,787 - Psyr_1710 0.61 -0.3 1,936,792 + Psyr_1710 0.61 +0.5 1,936,793 - Psyr_1710 0.61 +2.0 1,936,793 - Psyr_1710 0.61 +3.7 1,936,793 - Psyr_1710 0.61 -0.2 1,936,793 - Psyr_1710 0.61 +2.8 1,936,793 - Psyr_1710 0.61 -1.4 1,937,173 + -0.9 1,937,174 - -1.7 1,937,231 - -2.1 1,937,239 - -1.9 1,937,255 + -1.1 1,937,255 + +2.8 1,937,256 - -0.9 1,937,256 - -1.2 1,937,316 + -1.0 1,937,316 + +2.6 1,937,316 + +3.3 1,937,448 - Psyr_1711 0.49 -0.1 1,937,600 + -1.5 1,937,600 + -0.0 1,937,601 - -0.0 1,937,601 - -2.4 1,937,622 + -0.0 1,937,622 + -2.0 1,937,622 + -1.5 1,937,668 + -0.0 1,937,686 - +0.5 1,937,757 + +0.2 1,937,758 - +0.5 1,937,806 + Psyr_1712 0.12 -0.5 1,937,807 - Psyr_1712 0.12 +3.1 1,937,807 - Psyr_1712 0.12 -0.0 1,937,807 - Psyr_1712 0.12 -1.1 1,937,807 - Psyr_1712 0.12 +0.7 1,938,062 - Psyr_1712 0.34 +2.3 1,938,092 - Psyr_1712 0.36 -0.2 1,938,098 + Psyr_1712 0.37 -1.3
Or see this region's nucleotide sequence