Experiment: KB with Acridine orange 62.5 ug/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Psyr_1688 and Psyr_1689 are separated by 29 nucleotides Psyr_1689 and Psyr_1690 are separated by 12 nucleotides Psyr_1690 and Psyr_1691 are separated by 182 nucleotides Psyr_1691 and Psyr_1692 are separated by 12 nucleotides  
        
        Psyr_1688: Psyr_1688 - Protein of unknown function DUF1315, at 1,896,638 to 1,896,898 
         
        _1688 
         
        
        Psyr_1689: Psyr_1689 - conserved hypothetical protein, at 1,896,928 to 1,897,728 
         
        _1689 
         
        
        Psyr_1690: Psyr_1690 - Peptidase M1, membrane alanine aminopeptidase, at 1,897,741 to 1,900,407 
         
        _1690 
         
        
        Psyr_1691: Psyr_1691 - hypothetical protein, at 1,900,590 to 1,900,832 
         
        _1691 
         
        
        Psyr_1692: Psyr_1692 - conserved hypothetical protein, at 1,900,845 to 1,901,498 
         
        _1692 
         Position (kb)  
1897 
 
1898 
 
1899 
 
1900 
 
1901 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 
 
4 at 1896.964 kb on + strand at 1897.084 kb on + strand, within Psyr_1689 at 1897.096 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.535 kb on + strand, within Psyr_1689 at 1897.535 kb on + strand, within Psyr_1689 at 1897.536 kb on - strand, within Psyr_1689 at 1897.536 kb on - strand, within Psyr_1689 at 1897.658 kb on - strand at 1897.658 kb on - strand at 1897.696 kb on + strand at 1897.697 kb on - strand at 1897.697 kb on - strand at 1897.706 kb on - strand at 1897.706 kb on - strand at 1897.706 kb on - strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.727 kb on - strand at 1897.727 kb on - strand at 1897.768 kb on + strand at 1897.780 kb on + strand at 1897.781 kb on - strand at 1897.796 kb on - strand at 1898.086 kb on + strand, within Psyr_1690 at 1898.086 kb on + strand, within Psyr_1690 at 1898.086 kb on + strand, within Psyr_1690 at 1898.086 kb on + strand, within Psyr_1690 at 1898.086 kb on + strand, within Psyr_1690 at 1898.086 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.146 kb on + strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.147 kb on - strand, within Psyr_1690 at 1898.149 kb on + strand, within Psyr_1690 at 1898.367 kb on + strand, within Psyr_1690 at 1898.367 kb on + strand, within Psyr_1690 at 1898.368 kb on - strand, within Psyr_1690 at 1898.368 kb on - strand, within Psyr_1690 at 1898.395 kb on - strand, within Psyr_1690 at 1898.395 kb on - strand, within Psyr_1690 at 1898.398 kb on + strand, within Psyr_1690 at 1898.398 kb on + strand, within Psyr_1690 at 1898.398 kb on + strand, within Psyr_1690 at 1898.398 kb on + strand, within Psyr_1690 at 1898.399 kb on - strand, within Psyr_1690 at 1898.479 kb on + strand, within Psyr_1690 at 1898.479 kb on + strand, within Psyr_1690 at 1898.479 kb on + strand, within Psyr_1690 at 1898.565 kb on + strand, within Psyr_1690 at 1898.566 kb on - strand, within Psyr_1690 at 1898.566 kb on - strand, within Psyr_1690 at 1898.653 kb on + strand, within Psyr_1690 at 1898.653 kb on + strand, within Psyr_1690 at 1898.732 kb on - strand, within Psyr_1690 at 1898.798 kb on - strand, within Psyr_1690 at 1898.805 kb on + strand, within Psyr_1690 at 1898.806 kb on - strand, within Psyr_1690 at 1898.884 kb on + strand, within Psyr_1690 at 1898.885 kb on - strand, within Psyr_1690 at 1898.885 kb on - strand, within Psyr_1690 at 1898.977 kb on + strand, within Psyr_1690 at 1898.978 kb on - strand, within Psyr_1690 at 1899.076 kb on + strand, within Psyr_1690 at 1899.118 kb on + strand, within Psyr_1690 at 1899.119 kb on - strand, within Psyr_1690 at 1899.139 kb on + strand, within Psyr_1690 at 1899.139 kb on + strand, within Psyr_1690 at 1899.295 kb on - strand, within Psyr_1690 at 1899.500 kb on + strand, within Psyr_1690 at 1899.646 kb on + strand, within Psyr_1690 at 1899.646 kb on + strand, within Psyr_1690 at 1899.647 kb on - strand, within Psyr_1690 at 1899.647 kb on - strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.790 kb on + strand, within Psyr_1690 at 1899.791 kb on - strand, within Psyr_1690 at 1899.791 kb on - strand, within Psyr_1690 at 1899.791 kb on - strand, within Psyr_1690 at 1899.791 kb on - strand, within Psyr_1690 at 1900.375 kb on + strand at 1900.375 kb on + strand at 1900.376 kb on - strand at 1900.376 kb on - strand at 1900.376 kb on - strand at 1900.584 kb on + strand at 1900.585 kb on - strand at 1900.628 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.713 kb on - strand, within Psyr_1691 at 1900.734 kb on + strand, within Psyr_1691 at 1900.734 kb on + strand, within Psyr_1691 at 1900.777 kb on + strand, within Psyr_1691 at 1900.777 kb on + strand, within Psyr_1691 at 1900.781 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.809 kb on + strand at 1900.809 kb on + strand at 1900.810 kb on - strand at 1900.854 kb on - strand at 1900.854 kb on - strand at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1901.045 kb on - strand, within Psyr_1692 at 1901.045 kb on - strand, within Psyr_1692 at 1901.073 kb on - strand, within Psyr_1692 at 1901.196 kb on + strand, within Psyr_1692 at 1901.214 kb on + strand, within Psyr_1692 at 1901.214 kb on + strand, within Psyr_1692 at 1901.215 kb on - strand, within Psyr_1692 at 1901.215 kb on - strand, within Psyr_1692 at 1901.224 kb on + strand, within Psyr_1692 at 1901.225 kb on - strand, within Psyr_1692  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   KB with Acridine orange 62.5 ug/ml             remove   1,896,964   +        -0.7   1,897,084   +    Psyr_1689   0.19  -1.8   1,897,096   +    Psyr_1689   0.21  +4.4   1,897,231   +    Psyr_1689   0.38  -0.6   1,897,231   +    Psyr_1689   0.38  -1.2   1,897,231   +    Psyr_1689   0.38  +3.6   1,897,535   +    Psyr_1689   0.76  -0.3   1,897,535   +    Psyr_1689   0.76  +1.0   1,897,536   -    Psyr_1689   0.76  +3.0   1,897,536   -    Psyr_1689   0.76  +2.4   1,897,658   -        +2.8   1,897,658   -        -0.5   1,897,696   +        +2.8   1,897,697   -        -0.8   1,897,697   -        +0.7   1,897,706   -        -2.4   1,897,706   -        -1.3   1,897,706   -        +4.4   1,897,726   +        -0.9   1,897,726   +        +1.6   1,897,726   +        -0.9   1,897,726   +        +2.0   1,897,726   +        -0.3   1,897,727   -        +2.8   1,897,727   -        -1.3   1,897,768   +        -2.2   1,897,780   +        +0.9   1,897,781   -        +2.0   1,897,796   -        -1.7   1,898,086   +    Psyr_1690   0.13  +0.5   1,898,086   +    Psyr_1690   0.13  +0.8   1,898,086   +    Psyr_1690   0.13  -2.1   1,898,086   +    Psyr_1690   0.13  -0.3   1,898,086   +    Psyr_1690   0.13  +0.3   1,898,086   +    Psyr_1690   0.13  +1.2   1,898,146   +    Psyr_1690   0.15  -3.1   1,898,146   +    Psyr_1690   0.15  +4.2   1,898,146   +    Psyr_1690   0.15  +3.0   1,898,146   +    Psyr_1690   0.15  +2.3   1,898,146   +    Psyr_1690   0.15  +0.4   1,898,146   +    Psyr_1690   0.15  -0.3   1,898,146   +    Psyr_1690   0.15  +3.6   1,898,146   +    Psyr_1690   0.15  -0.2   1,898,147   -    Psyr_1690   0.15  +1.6   1,898,147   -    Psyr_1690   0.15  -1.9   1,898,147   -    Psyr_1690   0.15  -0.4   1,898,147   -    Psyr_1690   0.15  -1.9   1,898,147   -    Psyr_1690   0.15  -1.6   1,898,147   -    Psyr_1690   0.15  +3.2   1,898,147   -    Psyr_1690   0.15  +3.2   1,898,147   -    Psyr_1690   0.15  -1.2   1,898,147   -    Psyr_1690   0.15  +3.3   1,898,147   -    Psyr_1690   0.15  -2.1   1,898,147   -    Psyr_1690   0.15  -3.5   1,898,149   +    Psyr_1690   0.15  +3.0   1,898,367   +    Psyr_1690   0.23  +3.6   1,898,367   +    Psyr_1690   0.23  +3.0   1,898,368   -    Psyr_1690   0.24  +3.0   1,898,368   -    Psyr_1690   0.24  +2.0   1,898,395   -    Psyr_1690   0.25  -2.3   1,898,395   -    Psyr_1690   0.25  +2.6   1,898,398   +    Psyr_1690   0.25  -2.2   1,898,398   +    Psyr_1690   0.25  -1.7   1,898,398   +    Psyr_1690   0.25  +3.9   1,898,398   +    Psyr_1690   0.25  +2.0   1,898,399   -    Psyr_1690   0.25  +2.8   1,898,479   +    Psyr_1690   0.28  +3.7   1,898,479   +    Psyr_1690   0.28  +2.3   1,898,479   +    Psyr_1690   0.28  -0.9   1,898,565   +    Psyr_1690   0.31  +1.0   1,898,566   -    Psyr_1690   0.31  -0.4   1,898,566   -    Psyr_1690   0.31  -1.8   1,898,653   +    Psyr_1690   0.34  +0.8   1,898,653   +    Psyr_1690   0.34  +2.6   1,898,732   -    Psyr_1690   0.37  -1.0   1,898,798   -    Psyr_1690   0.40  -2.1   1,898,805   +    Psyr_1690   0.40  +0.7   1,898,806   -    Psyr_1690   0.40  -1.0   1,898,884   +    Psyr_1690   0.43  -1.0   1,898,885   -    Psyr_1690   0.43  -0.6   1,898,885   -    Psyr_1690   0.43  +2.0   1,898,977   +    Psyr_1690   0.46  +0.9   1,898,978   -    Psyr_1690   0.46  +3.3   1,899,076   +    Psyr_1690   0.50  +2.3   1,899,118   +    Psyr_1690   0.52  +1.1   1,899,119   -    Psyr_1690   0.52  -0.9   1,899,139   +    Psyr_1690   0.52  +2.6   1,899,139   +    Psyr_1690   0.52  +0.9   1,899,295   -    Psyr_1690   0.58  +3.7   1,899,500   +    Psyr_1690   0.66  -0.6   1,899,646   +    Psyr_1690   0.71  +0.8   1,899,646   +    Psyr_1690   0.71  -1.2   1,899,647   -    Psyr_1690   0.71  +3.8   1,899,647   -    Psyr_1690   0.71  +1.6   1,899,790   +    Psyr_1690   0.77  +1.0   1,899,790   +    Psyr_1690   0.77  +3.0   1,899,790   +    Psyr_1690   0.77  +2.0   1,899,790   +    Psyr_1690   0.77  -1.8   1,899,790   +    Psyr_1690   0.77  +2.6   1,899,790   +    Psyr_1690   0.77  -3.1   1,899,791   -    Psyr_1690   0.77  -2.3   1,899,791   -    Psyr_1690   0.77  +0.2   1,899,791   -    Psyr_1690   0.77  +1.6   1,899,791   -    Psyr_1690   0.77  -1.4   1,900,375   +        +2.3   1,900,375   +        -2.1   1,900,376   -        -0.6   1,900,376   -        +1.0   1,900,376   -        -0.7   1,900,584   +        -2.4   1,900,585   -        +3.7   1,900,628   +    Psyr_1691   0.16  +1.9   1,900,712   +    Psyr_1691   0.50  -2.8   1,900,712   +    Psyr_1691   0.50  +1.0   1,900,712   +    Psyr_1691   0.50  +3.2   1,900,712   +    Psyr_1691   0.50  -0.9   1,900,712   +    Psyr_1691   0.50  +2.3   1,900,713   -    Psyr_1691   0.51  +2.3   1,900,734   +    Psyr_1691   0.59  -2.2   1,900,734   +    Psyr_1691   0.59  -1.0   1,900,777   +    Psyr_1691   0.77  +2.3   1,900,777   +    Psyr_1691   0.77  +4.1   1,900,781   +    Psyr_1691   0.79  -0.0   1,900,800   +    Psyr_1691   0.86  +0.8   1,900,800   +    Psyr_1691   0.86  -1.6   1,900,800   +    Psyr_1691   0.86  -2.2   1,900,801   -    Psyr_1691   0.87  +2.0   1,900,801   -    Psyr_1691   0.87  +4.5   1,900,801   -    Psyr_1691   0.87  -0.8   1,900,809   +        +0.1   1,900,809   +        -0.4   1,900,810   -        -0.9   1,900,854   -        -0.7   1,900,854   -        +0.2   1,900,911   +    Psyr_1692   0.10  +2.0   1,900,911   +    Psyr_1692   0.10  -1.2   1,900,911   +    Psyr_1692   0.10  +0.3   1,900,911   +    Psyr_1692   0.10  +3.6   1,900,912   -    Psyr_1692   0.10  +3.4   1,900,912   -    Psyr_1692   0.10  +3.6   1,900,912   -    Psyr_1692   0.10  -1.4   1,900,912   -    Psyr_1692   0.10  +4.1   1,901,045   -    Psyr_1692   0.31  -3.2   1,901,045   -    Psyr_1692   0.31  +3.3   1,901,073   -    Psyr_1692   0.35  -1.3   1,901,196   +    Psyr_1692   0.54  +3.1   1,901,214   +    Psyr_1692   0.56  +1.0   1,901,214   +    Psyr_1692   0.56  +3.1   1,901,215   -    Psyr_1692   0.57  +2.6   1,901,215   -    Psyr_1692   0.57  -0.0   1,901,224   +    Psyr_1692   0.58  +3.6   1,901,225   -    Psyr_1692   0.58  -0.0 
 
Or see this region's nucleotide sequence