Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1665 and Psyr_1666 overlap by 17 nucleotides Psyr_1666 and Psyr_1667 are separated by 118 nucleotides Psyr_1667 and Psyr_1668 are separated by 12 nucleotides
Psyr_1665: Psyr_1665 - Folylpolyglutamate synthetase, at 1,869,795 to 1,871,099
_1665
Psyr_1666: Psyr_1666 - Sporulation related protein, at 1,871,083 to 1,871,736
_1666
Psyr_1667: Psyr_1667 - Colicin V production protein, at 1,871,855 to 1,872,442
_1667
Psyr_1668: Psyr_1668 - amidophosphoribosyltransferase, at 1,872,455 to 1,873,963
_1668
Position (kb)
1871
1872
1873 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.584 kb on + strand, within Psyr_1666 at 1871.584 kb on + strand, within Psyr_1666 at 1871.584 kb on + strand, within Psyr_1666 at 1871.585 kb on - strand, within Psyr_1666 at 1871.593 kb on + strand, within Psyr_1666 at 1871.593 kb on + strand, within Psyr_1666 at 1871.594 kb on - strand, within Psyr_1666 at 1871.594 kb on - strand, within Psyr_1666 at 1871.703 kb on + strand at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.024 kb on - strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.028 kb on - strand, within Psyr_1667 at 1872.028 kb on - strand, within Psyr_1667 at 1872.061 kb on + strand, within Psyr_1667 at 1872.114 kb on + strand, within Psyr_1667 at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.398 kb on - strand at 1872.398 kb on - strand at 1872.436 kb on + strand at 1872.437 kb on - strand at 1872.437 kb on - strand at 1872.506 kb on + strand at 1872.506 kb on + strand at 1872.506 kb on + strand at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.653 kb on - strand, within Psyr_1668 at 1872.908 kb on - strand, within Psyr_1668 at 1873.021 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.062 kb on - strand, within Psyr_1668 at 1873.062 kb on - strand, within Psyr_1668 at 1873.142 kb on + strand, within Psyr_1668 at 1873.142 kb on + strand, within Psyr_1668 at 1873.143 kb on - strand, within Psyr_1668 at 1873.197 kb on - strand, within Psyr_1668 at 1873.197 kb on - strand, within Psyr_1668 at 1873.224 kb on - strand, within Psyr_1668 at 1873.224 kb on - strand, within Psyr_1668 at 1873.256 kb on - strand, within Psyr_1668 at 1873.265 kb on + strand, within Psyr_1668 at 1873.265 kb on + strand, within Psyr_1668 at 1873.266 kb on - strand, within Psyr_1668 at 1873.436 kb on + strand, within Psyr_1668 at 1873.436 kb on + strand, within Psyr_1668 at 1873.437 kb on - strand, within Psyr_1668 at 1873.437 kb on - strand, within Psyr_1668
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,871,102 + -1.3 1,871,102 + -2.1 1,871,102 + -3.5 1,871,102 + +2.6 1,871,102 + -2.0 1,871,102 + -1.0 1,871,102 + -2.1 1,871,103 - -0.0 1,871,103 - -3.2 1,871,103 - -1.9 1,871,103 - +0.3 1,871,103 - -5.6 1,871,103 - -4.3 1,871,103 - -3.7 1,871,103 - -1.6 1,871,103 - -0.0 1,871,103 - -4.1 1,871,584 + Psyr_1666 0.77 -0.7 1,871,584 + Psyr_1666 0.77 +0.4 1,871,584 + Psyr_1666 0.77 -1.5 1,871,585 - Psyr_1666 0.77 -0.0 1,871,593 + Psyr_1666 0.78 -0.6 1,871,593 + Psyr_1666 0.78 +3.7 1,871,594 - Psyr_1666 0.78 +0.6 1,871,594 - Psyr_1666 0.78 +0.6 1,871,703 + +3.0 1,872,023 + Psyr_1667 0.29 +2.8 1,872,023 + Psyr_1667 0.29 -1.2 1,872,023 + Psyr_1667 0.29 -1.9 1,872,023 + Psyr_1667 0.29 -0.0 1,872,023 + Psyr_1667 0.29 -2.2 1,872,023 + Psyr_1667 0.29 -2.3 1,872,024 - Psyr_1667 0.29 -5.0 1,872,027 + Psyr_1667 0.29 -0.3 1,872,027 + Psyr_1667 0.29 -2.9 1,872,027 + Psyr_1667 0.29 +2.3 1,872,027 + Psyr_1667 0.29 -0.0 1,872,028 - Psyr_1667 0.29 -0.0 1,872,028 - Psyr_1667 0.29 -0.0 1,872,061 + Psyr_1667 0.35 -1.5 1,872,114 + Psyr_1667 0.44 -0.4 1,872,397 + -1.0 1,872,397 + -2.0 1,872,397 + +1.3 1,872,397 + +3.8 1,872,398 - -2.8 1,872,398 - +3.2 1,872,436 + -0.8 1,872,437 - +3.6 1,872,437 - +1.6 1,872,506 + +2.3 1,872,506 + +3.0 1,872,506 + +2.8 1,872,631 + Psyr_1668 0.12 -1.3 1,872,631 + Psyr_1668 0.12 +2.3 1,872,631 + Psyr_1668 0.12 +2.3 1,872,631 + Psyr_1668 0.12 +3.8 1,872,632 - Psyr_1668 0.12 -1.1 1,872,632 - Psyr_1668 0.12 +2.3 1,872,632 - Psyr_1668 0.12 -0.0 1,872,632 - Psyr_1668 0.12 +1.6 1,872,653 - Psyr_1668 0.13 -0.0 1,872,908 - Psyr_1668 0.30 +1.6 1,873,021 + Psyr_1668 0.38 -1.8 1,873,061 + Psyr_1668 0.40 -0.5 1,873,061 + Psyr_1668 0.40 +2.3 1,873,061 + Psyr_1668 0.40 -0.7 1,873,062 - Psyr_1668 0.40 -0.0 1,873,062 - Psyr_1668 0.40 -3.3 1,873,142 + Psyr_1668 0.46 +1.0 1,873,142 + Psyr_1668 0.46 -4.1 1,873,143 - Psyr_1668 0.46 +3.6 1,873,197 - Psyr_1668 0.49 -0.3 1,873,197 - Psyr_1668 0.49 -3.3 1,873,224 - Psyr_1668 0.51 -0.9 1,873,224 - Psyr_1668 0.51 +3.6 1,873,256 - Psyr_1668 0.53 -2.2 1,873,265 + Psyr_1668 0.54 -0.0 1,873,265 + Psyr_1668 0.54 -1.7 1,873,266 - Psyr_1668 0.54 -1.4 1,873,436 + Psyr_1668 0.65 +2.0 1,873,436 + Psyr_1668 0.65 +1.6 1,873,437 - Psyr_1668 0.65 -0.0 1,873,437 - Psyr_1668 0.65 -1.6
Or see this region's nucleotide sequence