Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1620 and Psyr_1621 are separated by 219 nucleotides Psyr_1621 and Psyr_1622 are separated by 1 nucleotides Psyr_1622 and Psyr_1623 are separated by 287 nucleotides
Psyr_1620: Psyr_1620 - conserved hypothetical protein, at 1,821,315 to 1,822,511
_1620
Psyr_1621: Psyr_1621 - Autoinducer synthesis protein, at 1,822,731 to 1,823,411
_1621
Psyr_1622: Psyr_1622 - regulatory protein, LuxR:Autoinducer-binding protein, at 1,823,413 to 1,824,156
_1622
Psyr_1623: Psyr_1623 - Pyruvate dehydrogenase (lipoamide), at 1,824,444 to 1,825,547
_1623
Position (kb) 1823
1824
1825 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1822.455 kb on - strand at 1822.499 kb on - strand at 1822.499 kb on - strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.506 kb on - strand at 1822.506 kb on - strand at 1822.611 kb on + strand at 1822.611 kb on + strand at 1822.612 kb on - strand at 1822.612 kb on - strand at 1822.615 kb on + strand at 1822.615 kb on + strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.663 kb on + strand at 1822.667 kb on + strand at 1822.673 kb on - strand at 1822.691 kb on - strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.727 kb on + strand at 1822.728 kb on - strand at 1822.764 kb on + strand at 1822.764 kb on + strand at 1822.800 kb on + strand, within Psyr_1621 at 1822.801 kb on - strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1823.255 kb on - strand, within Psyr_1621 at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.428 kb on - strand at 1823.431 kb on - strand at 1823.441 kb on + strand at 1823.441 kb on + strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.546 kb on + strand, within Psyr_1622 at 1823.582 kb on - strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.811 kb on - strand, within Psyr_1622 at 1823.895 kb on + strand, within Psyr_1622 at 1823.896 kb on - strand, within Psyr_1622 at 1823.925 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.934 kb on - strand, within Psyr_1622 at 1823.934 kb on - strand, within Psyr_1622 at 1823.947 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1824.023 kb on + strand, within Psyr_1622 at 1824.024 kb on - strand, within Psyr_1622 at 1824.024 kb on - strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.046 kb on - strand, within Psyr_1622 at 1824.144 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.161 kb on + strand at 1824.162 kb on - strand at 1824.162 kb on - strand at 1824.162 kb on - strand at 1824.170 kb on + strand at 1824.170 kb on + strand at 1824.171 kb on - strand at 1824.225 kb on + strand at 1824.286 kb on + strand at 1824.286 kb on + strand at 1824.374 kb on - strand at 1824.390 kb on + strand at 1824.390 kb on + strand at 1824.390 kb on + strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.439 kb on + strand at 1824.439 kb on + strand at 1824.439 kb on + strand at 1824.440 kb on - strand at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.733 kb on - strand, within Psyr_1623 at 1824.957 kb on + strand, within Psyr_1623 at 1824.958 kb on - strand, within Psyr_1623 at 1824.958 kb on - strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1825.108 kb on + strand, within Psyr_1623
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,822,455 - -0.0 1,822,499 - -2.1 1,822,499 - -2.9 1,822,505 + -0.2 1,822,505 + +0.6 1,822,505 + -0.0 1,822,506 - +0.6 1,822,506 - -0.1 1,822,611 + -0.0 1,822,611 + +2.2 1,822,612 - +2.3 1,822,612 - +2.8 1,822,615 + +0.5 1,822,615 + -1.9 1,822,616 - +1.4 1,822,616 - +2.3 1,822,616 - +1.6 1,822,663 + -2.9 1,822,667 + -1.1 1,822,673 - +3.0 1,822,691 - -0.0 1,822,694 + +1.6 1,822,694 + -0.9 1,822,694 + +3.2 1,822,694 + +4.0 1,822,727 + -0.6 1,822,728 - -1.2 1,822,764 + -3.4 1,822,764 + -1.0 1,822,800 + Psyr_1621 0.10 +0.6 1,822,801 - Psyr_1621 0.10 +2.3 1,822,878 + Psyr_1621 0.22 +3.0 1,822,878 + Psyr_1621 0.22 +3.2 1,822,878 + Psyr_1621 0.22 +2.8 1,822,878 + Psyr_1621 0.22 -0.3 1,822,879 - Psyr_1621 0.22 +2.6 1,822,879 - Psyr_1621 0.22 -0.9 1,822,899 + Psyr_1621 0.25 -0.0 1,822,899 + Psyr_1621 0.25 -0.3 1,822,965 + Psyr_1621 0.34 +1.0 1,822,965 + Psyr_1621 0.34 -1.4 1,823,255 - Psyr_1621 0.77 +1.0 1,823,427 + -1.4 1,823,427 + -0.5 1,823,427 + +0.5 1,823,428 - +1.4 1,823,431 - -1.9 1,823,441 + -0.0 1,823,441 + +2.1 1,823,442 - -0.0 1,823,442 - -0.0 1,823,442 - -2.3 1,823,442 - +2.8 1,823,546 + Psyr_1622 0.18 +4.8 1,823,582 - Psyr_1622 0.23 -2.5 1,823,810 + Psyr_1622 0.53 -0.3 1,823,810 + Psyr_1622 0.53 +1.6 1,823,810 + Psyr_1622 0.53 -0.9 1,823,810 + Psyr_1622 0.53 -0.6 1,823,811 - Psyr_1622 0.53 -1.2 1,823,895 + Psyr_1622 0.65 +0.8 1,823,896 - Psyr_1622 0.65 +1.6 1,823,925 + Psyr_1622 0.69 -0.8 1,823,933 + Psyr_1622 0.70 +2.6 1,823,933 + Psyr_1622 0.70 -0.2 1,823,933 + Psyr_1622 0.70 +4.0 1,823,933 + Psyr_1622 0.70 +2.8 1,823,934 - Psyr_1622 0.70 -3.8 1,823,934 - Psyr_1622 0.70 -1.9 1,823,947 - Psyr_1622 0.72 -0.0 1,823,949 - Psyr_1622 0.72 +1.6 1,823,949 - Psyr_1622 0.72 -0.5 1,823,949 - Psyr_1622 0.72 -0.0 1,823,949 - Psyr_1622 0.72 -1.9 1,823,972 + Psyr_1622 0.75 -0.8 1,823,972 + Psyr_1622 0.75 -0.8 1,823,972 + Psyr_1622 0.75 -5.3 1,824,023 + Psyr_1622 0.82 -0.0 1,824,024 - Psyr_1622 0.82 +1.6 1,824,024 - Psyr_1622 0.82 -4.0 1,824,029 + Psyr_1622 0.83 +1.0 1,824,029 + Psyr_1622 0.83 +3.8 1,824,029 + Psyr_1622 0.83 +0.7 1,824,029 + Psyr_1622 0.83 +3.7 1,824,030 - Psyr_1622 0.83 -3.3 1,824,030 - Psyr_1622 0.83 -0.0 1,824,030 - Psyr_1622 0.83 +4.0 1,824,030 - Psyr_1622 0.83 -4.1 1,824,046 - Psyr_1622 0.85 -2.8 1,824,144 + -0.4 1,824,158 + +1.6 1,824,158 + -2.6 1,824,158 + +1.6 1,824,158 + -1.9 1,824,158 + -1.6 1,824,158 + +3.8 1,824,158 + +1.6 1,824,158 + -0.9 1,824,159 - -0.0 1,824,159 - -3.0 1,824,159 - -1.0 1,824,159 - -3.9 1,824,159 - +2.3 1,824,161 + +0.1 1,824,162 - -0.0 1,824,162 - +1.6 1,824,162 - +1.6 1,824,170 + +1.6 1,824,170 + -0.8 1,824,171 - -1.1 1,824,225 + +3.2 1,824,286 + -3.0 1,824,286 + -2.1 1,824,374 - +1.6 1,824,390 + -2.0 1,824,390 + -0.9 1,824,390 + -1.0 1,824,391 - -2.1 1,824,391 - -1.0 1,824,391 - -2.6 1,824,391 - +1.0 1,824,439 + -0.0 1,824,439 + +3.6 1,824,439 + +2.8 1,824,440 - +2.0 1,824,732 + Psyr_1623 0.26 -0.2 1,824,732 + Psyr_1623 0.26 -1.4 1,824,732 + Psyr_1623 0.26 +1.6 1,824,732 + Psyr_1623 0.26 +2.3 1,824,733 - Psyr_1623 0.26 +0.8 1,824,957 + Psyr_1623 0.46 +3.3 1,824,958 - Psyr_1623 0.47 -0.8 1,824,958 - Psyr_1623 0.47 +2.0 1,824,981 + Psyr_1623 0.49 -0.8 1,824,981 + Psyr_1623 0.49 -0.0 1,824,981 + Psyr_1623 0.49 +2.0 1,824,982 - Psyr_1623 0.49 +3.7 1,824,982 - Psyr_1623 0.49 +1.6 1,824,982 - Psyr_1623 0.49 +4.4 1,824,984 + Psyr_1623 0.49 -0.6 1,824,984 + Psyr_1623 0.49 -0.1 1,824,984 + Psyr_1623 0.49 -2.6 1,824,984 + Psyr_1623 0.49 +0.6 1,824,984 + Psyr_1623 0.49 +1.6 1,824,984 + Psyr_1623 0.49 -0.0 1,824,984 + Psyr_1623 0.49 +2.8 1,824,984 + Psyr_1623 0.49 -0.4 1,824,984 + Psyr_1623 0.49 +0.5 1,824,985 - Psyr_1623 0.49 +1.0 1,824,985 - Psyr_1623 0.49 -0.1 1,824,985 - Psyr_1623 0.49 -0.0 1,824,985 - Psyr_1623 0.49 +2.6 1,824,985 - Psyr_1623 0.49 -0.7 1,824,985 - Psyr_1623 0.49 -0.3 1,824,985 - Psyr_1623 0.49 -1.6 1,824,985 - Psyr_1623 0.49 -0.2 1,824,985 - Psyr_1623 0.49 +2.6 1,824,985 - -0.0 1,824,985 - Psyr_1623 0.49 -1.2 1,824,985 - Psyr_1623 0.49 +2.0 1,824,985 - Psyr_1623 0.49 +1.6 1,824,985 - Psyr_1623 0.49 +1.0 1,825,108 + Psyr_1623 0.60 -4.0
Or see this region's nucleotide sequence