Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1618 and Psyr_1619 overlap by 11 nucleotides Psyr_1619 and Psyr_1620 overlap by 8 nucleotides Psyr_1620 and Psyr_1621 are separated by 219 nucleotides Psyr_1621 and Psyr_1622 are separated by 1 nucleotides
Psyr_1618: Psyr_1618 - Dihydrodipicolinate synthetase, at 1,820,017 to 1,820,814
_1618
Psyr_1619: Psyr_1619 - conserved hypothetical protein, at 1,820,804 to 1,821,322
_1619
Psyr_1620: Psyr_1620 - conserved hypothetical protein, at 1,821,315 to 1,822,511
_1620
Psyr_1621: Psyr_1621 - Autoinducer synthesis protein, at 1,822,731 to 1,823,411
_1621
Psyr_1622: Psyr_1622 - regulatory protein, LuxR:Autoinducer-binding protein, at 1,823,413 to 1,824,156
_1622
Position (kb)
1821
1822
1823 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.420 kb on + strand, within Psyr_1618 at 1820.421 kb on - strand, within Psyr_1618 at 1820.421 kb on - strand, within Psyr_1618 at 1820.431 kb on - strand, within Psyr_1618 at 1820.511 kb on + strand, within Psyr_1618 at 1820.511 kb on + strand, within Psyr_1618 at 1820.511 kb on + strand, within Psyr_1618 at 1820.512 kb on - strand, within Psyr_1618 at 1820.512 kb on - strand, within Psyr_1618 at 1820.512 kb on - strand, within Psyr_1618 at 1820.512 kb on - strand, within Psyr_1618 at 1820.699 kb on + strand, within Psyr_1618 at 1820.699 kb on + strand, within Psyr_1618 at 1820.805 kb on + strand at 1820.859 kb on + strand, within Psyr_1619 at 1820.859 kb on + strand, within Psyr_1619 at 1820.859 kb on + strand, within Psyr_1619 at 1820.859 kb on + strand, within Psyr_1619 at 1820.860 kb on - strand, within Psyr_1619 at 1820.966 kb on - strand, within Psyr_1619 at 1820.966 kb on - strand, within Psyr_1619 at 1820.966 kb on - strand, within Psyr_1619 at 1820.968 kb on - strand, within Psyr_1619 at 1821.074 kb on - strand, within Psyr_1619 at 1821.075 kb on + strand, within Psyr_1619 at 1821.076 kb on - strand, within Psyr_1619 at 1821.076 kb on - strand, within Psyr_1619 at 1821.076 kb on - strand, within Psyr_1619 at 1821.089 kb on + strand, within Psyr_1619 at 1821.129 kb on + strand, within Psyr_1619 at 1821.129 kb on + strand, within Psyr_1619 at 1821.129 kb on + strand, within Psyr_1619 at 1821.129 kb on + strand, within Psyr_1619 at 1821.130 kb on - strand, within Psyr_1619 at 1821.183 kb on - strand, within Psyr_1619 at 1821.216 kb on + strand, within Psyr_1619 at 1821.216 kb on + strand, within Psyr_1619 at 1821.216 kb on + strand, within Psyr_1619 at 1821.217 kb on - strand, within Psyr_1619 at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.399 kb on + strand at 1821.400 kb on - strand at 1821.400 kb on - strand at 1821.400 kb on - strand at 1821.400 kb on - strand at 1821.400 kb on - strand at 1821.403 kb on + strand at 1821.404 kb on - strand at 1821.404 kb on - strand at 1821.404 kb on - strand at 1821.404 kb on - strand at 1821.439 kb on + strand, within Psyr_1620 at 1821.439 kb on + strand, within Psyr_1620 at 1821.439 kb on + strand, within Psyr_1620 at 1821.439 kb on + strand, within Psyr_1620 at 1821.439 kb on + strand, within Psyr_1620 at 1821.440 kb on - strand, within Psyr_1620 at 1821.440 kb on - strand, within Psyr_1620 at 1821.440 kb on - strand, within Psyr_1620 at 1821.440 kb on - strand, within Psyr_1620 at 1821.453 kb on + strand, within Psyr_1620 at 1821.457 kb on + strand, within Psyr_1620 at 1821.458 kb on - strand, within Psyr_1620 at 1821.478 kb on + strand, within Psyr_1620 at 1821.478 kb on + strand, within Psyr_1620 at 1821.478 kb on + strand, within Psyr_1620 at 1821.479 kb on - strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.559 kb on + strand, within Psyr_1620 at 1821.560 kb on - strand, within Psyr_1620 at 1821.560 kb on - strand, within Psyr_1620 at 1821.560 kb on - strand, within Psyr_1620 at 1821.560 kb on - strand, within Psyr_1620 at 1821.661 kb on + strand, within Psyr_1620 at 1821.662 kb on - strand, within Psyr_1620 at 1821.724 kb on + strand, within Psyr_1620 at 1821.724 kb on + strand, within Psyr_1620 at 1821.724 kb on + strand at 1821.724 kb on + strand, within Psyr_1620 at 1821.724 kb on + strand, within Psyr_1620 at 1821.724 kb on + strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.725 kb on - strand, within Psyr_1620 at 1821.801 kb on - strand, within Psyr_1620 at 1821.818 kb on - strand, within Psyr_1620 at 1821.848 kb on - strand, within Psyr_1620 at 1821.922 kb on + strand, within Psyr_1620 at 1821.922 kb on + strand, within Psyr_1620 at 1821.923 kb on - strand, within Psyr_1620 at 1821.923 kb on - strand, within Psyr_1620 at 1821.923 kb on - strand, within Psyr_1620 at 1822.054 kb on + strand, within Psyr_1620 at 1822.054 kb on + strand, within Psyr_1620 at 1822.054 kb on + strand, within Psyr_1620 at 1822.055 kb on - strand, within Psyr_1620 at 1822.055 kb on - strand, within Psyr_1620 at 1822.186 kb on + strand, within Psyr_1620 at 1822.186 kb on + strand, within Psyr_1620 at 1822.187 kb on - strand, within Psyr_1620 at 1822.187 kb on - strand, within Psyr_1620 at 1822.187 kb on - strand, within Psyr_1620 at 1822.187 kb on - strand, within Psyr_1620 at 1822.199 kb on + strand, within Psyr_1620 at 1822.199 kb on + strand, within Psyr_1620 at 1822.200 kb on - strand, within Psyr_1620 at 1822.200 kb on - strand, within Psyr_1620 at 1822.231 kb on + strand, within Psyr_1620 at 1822.232 kb on - strand, within Psyr_1620 at 1822.232 kb on - strand, within Psyr_1620 at 1822.232 kb on - strand, within Psyr_1620 at 1822.313 kb on - strand, within Psyr_1620 at 1822.455 kb on - strand at 1822.499 kb on - strand at 1822.499 kb on - strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.506 kb on - strand at 1822.506 kb on - strand at 1822.611 kb on + strand at 1822.611 kb on + strand at 1822.612 kb on - strand at 1822.612 kb on - strand at 1822.615 kb on + strand at 1822.615 kb on + strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.663 kb on + strand at 1822.667 kb on + strand at 1822.673 kb on - strand at 1822.691 kb on - strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.727 kb on + strand at 1822.728 kb on - strand at 1822.764 kb on + strand at 1822.764 kb on + strand at 1822.800 kb on + strand, within Psyr_1621 at 1822.801 kb on - strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1823.255 kb on - strand, within Psyr_1621 at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.428 kb on - strand at 1823.431 kb on - strand at 1823.441 kb on + strand at 1823.441 kb on + strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,820,420 + Psyr_1618 0.51 -0.5 1,820,420 + Psyr_1618 0.51 +0.2 1,820,420 + Psyr_1618 0.51 +3.8 1,820,420 + Psyr_1618 0.51 +1.3 1,820,420 + Psyr_1618 0.51 +2.6 1,820,420 + Psyr_1618 0.51 +2.3 1,820,420 + Psyr_1618 0.51 +0.6 1,820,420 + Psyr_1618 0.51 +0.0 1,820,420 + Psyr_1618 0.51 +2.0 1,820,420 + Psyr_1618 0.51 +0.4 1,820,421 - Psyr_1618 0.51 +0.5 1,820,421 - Psyr_1618 0.51 -3.6 1,820,431 - Psyr_1618 0.52 -0.3 1,820,511 + Psyr_1618 0.62 -1.0 1,820,511 + Psyr_1618 0.62 -1.9 1,820,511 + Psyr_1618 0.62 +2.6 1,820,512 - Psyr_1618 0.62 -1.4 1,820,512 - Psyr_1618 0.62 -1.3 1,820,512 - Psyr_1618 0.62 +2.3 1,820,512 - Psyr_1618 0.62 -0.6 1,820,699 + Psyr_1618 0.85 -0.6 1,820,699 + Psyr_1618 0.85 +0.0 1,820,805 + +2.6 1,820,859 + Psyr_1619 0.11 +3.2 1,820,859 + Psyr_1619 0.11 -0.0 1,820,859 + Psyr_1619 0.11 -0.6 1,820,859 + Psyr_1619 0.11 -2.6 1,820,860 - Psyr_1619 0.11 +1.0 1,820,966 - Psyr_1619 0.31 +3.3 1,820,966 - Psyr_1619 0.31 +0.1 1,820,966 - Psyr_1619 0.31 +3.6 1,820,968 - Psyr_1619 0.32 +3.5 1,821,074 - Psyr_1619 0.52 +2.3 1,821,075 + Psyr_1619 0.52 +2.6 1,821,076 - Psyr_1619 0.52 -1.1 1,821,076 - Psyr_1619 0.52 -1.4 1,821,076 - Psyr_1619 0.52 -0.9 1,821,089 + Psyr_1619 0.55 +2.5 1,821,129 + Psyr_1619 0.63 +3.2 1,821,129 + Psyr_1619 0.63 +3.6 1,821,129 + Psyr_1619 0.63 +1.0 1,821,129 + Psyr_1619 0.63 -0.4 1,821,130 - Psyr_1619 0.63 -2.5 1,821,183 - Psyr_1619 0.73 +3.3 1,821,216 + Psyr_1619 0.79 -3.0 1,821,216 + Psyr_1619 0.79 -0.4 1,821,216 + Psyr_1619 0.79 -0.0 1,821,217 - Psyr_1619 0.80 +1.4 1,821,399 + -1.0 1,821,399 + +0.3 1,821,399 + -1.1 1,821,399 + +0.3 1,821,399 + +1.0 1,821,399 + +0.6 1,821,399 + +0.1 1,821,400 - +2.8 1,821,400 - -2.5 1,821,400 - -3.3 1,821,400 - -1.0 1,821,400 - +3.5 1,821,403 + +3.2 1,821,404 - +1.0 1,821,404 - -2.1 1,821,404 - -2.3 1,821,404 - +4.1 1,821,439 + Psyr_1620 0.10 -2.1 1,821,439 + Psyr_1620 0.10 +0.7 1,821,439 + Psyr_1620 0.10 +1.0 1,821,439 + Psyr_1620 0.10 -0.0 1,821,439 + Psyr_1620 0.10 -1.2 1,821,440 - Psyr_1620 0.10 +3.7 1,821,440 - Psyr_1620 0.10 -0.0 1,821,440 - Psyr_1620 0.10 +0.2 1,821,440 - Psyr_1620 0.10 -1.1 1,821,453 + Psyr_1620 0.12 +1.0 1,821,457 + Psyr_1620 0.12 +3.2 1,821,458 - Psyr_1620 0.12 +1.6 1,821,478 + Psyr_1620 0.14 -0.6 1,821,478 + Psyr_1620 0.14 +3.0 1,821,478 + Psyr_1620 0.14 +1.4 1,821,479 - Psyr_1620 0.14 -1.7 1,821,559 + Psyr_1620 0.20 +2.2 1,821,559 + Psyr_1620 0.20 -0.0 1,821,559 + Psyr_1620 0.20 +2.8 1,821,559 + Psyr_1620 0.20 +2.8 1,821,559 + Psyr_1620 0.20 +2.0 1,821,559 + Psyr_1620 0.20 +0.1 1,821,559 + Psyr_1620 0.20 -3.4 1,821,559 + Psyr_1620 0.20 +2.0 1,821,560 - Psyr_1620 0.20 +2.0 1,821,560 - Psyr_1620 0.20 +2.6 1,821,560 - Psyr_1620 0.20 +1.6 1,821,560 - Psyr_1620 0.20 -0.0 1,821,661 + Psyr_1620 0.29 -2.2 1,821,662 - Psyr_1620 0.29 -2.7 1,821,724 + Psyr_1620 0.34 -2.0 1,821,724 + Psyr_1620 0.34 +2.8 1,821,724 + -0.0 1,821,724 + Psyr_1620 0.34 +2.3 1,821,724 + Psyr_1620 0.34 -0.9 1,821,724 + Psyr_1620 0.34 -2.8 1,821,725 - Psyr_1620 0.34 +2.6 1,821,725 - Psyr_1620 0.34 +3.3 1,821,725 - Psyr_1620 0.34 -2.3 1,821,725 - Psyr_1620 0.34 -0.7 1,821,725 - Psyr_1620 0.34 +2.8 1,821,725 - Psyr_1620 0.34 -0.0 1,821,801 - Psyr_1620 0.41 -1.2 1,821,818 - Psyr_1620 0.42 +3.5 1,821,848 - Psyr_1620 0.45 +1.0 1,821,922 + Psyr_1620 0.51 -3.7 1,821,922 + Psyr_1620 0.51 +1.6 1,821,923 - Psyr_1620 0.51 -3.4 1,821,923 - Psyr_1620 0.51 +1.9 1,821,923 - Psyr_1620 0.51 -2.2 1,822,054 + Psyr_1620 0.62 -1.9 1,822,054 + Psyr_1620 0.62 +1.0 1,822,054 + Psyr_1620 0.62 +2.8 1,822,055 - Psyr_1620 0.62 +2.6 1,822,055 - Psyr_1620 0.62 +2.0 1,822,186 + Psyr_1620 0.73 -0.3 1,822,186 + Psyr_1620 0.73 +0.4 1,822,187 - Psyr_1620 0.73 -0.3 1,822,187 - Psyr_1620 0.73 -1.1 1,822,187 - Psyr_1620 0.73 -2.7 1,822,187 - Psyr_1620 0.73 -1.2 1,822,199 + Psyr_1620 0.74 +0.4 1,822,199 + Psyr_1620 0.74 +3.5 1,822,200 - Psyr_1620 0.74 -1.8 1,822,200 - Psyr_1620 0.74 -1.2 1,822,231 + Psyr_1620 0.77 -1.4 1,822,232 - Psyr_1620 0.77 +2.6 1,822,232 - Psyr_1620 0.77 +2.0 1,822,232 - Psyr_1620 0.77 +0.7 1,822,313 - Psyr_1620 0.83 -0.0 1,822,455 - -0.0 1,822,499 - -2.1 1,822,499 - -2.9 1,822,505 + -0.2 1,822,505 + +0.6 1,822,505 + -0.0 1,822,506 - +0.6 1,822,506 - -0.1 1,822,611 + -0.0 1,822,611 + +2.2 1,822,612 - +2.3 1,822,612 - +2.8 1,822,615 + +0.5 1,822,615 + -1.9 1,822,616 - +1.4 1,822,616 - +2.3 1,822,616 - +1.6 1,822,663 + -2.9 1,822,667 + -1.1 1,822,673 - +3.0 1,822,691 - -0.0 1,822,694 + +1.6 1,822,694 + -0.9 1,822,694 + +3.2 1,822,694 + +4.0 1,822,727 + -0.6 1,822,728 - -1.2 1,822,764 + -3.4 1,822,764 + -1.0 1,822,800 + Psyr_1621 0.10 +0.6 1,822,801 - Psyr_1621 0.10 +2.3 1,822,878 + Psyr_1621 0.22 +3.0 1,822,878 + Psyr_1621 0.22 +3.2 1,822,878 + Psyr_1621 0.22 +2.8 1,822,878 + Psyr_1621 0.22 -0.3 1,822,879 - Psyr_1621 0.22 +2.6 1,822,879 - Psyr_1621 0.22 -0.9 1,822,899 + Psyr_1621 0.25 -0.0 1,822,899 + Psyr_1621 0.25 -0.3 1,822,965 + Psyr_1621 0.34 +1.0 1,822,965 + Psyr_1621 0.34 -1.4 1,823,255 - Psyr_1621 0.77 +1.0 1,823,427 + -1.4 1,823,427 + -0.5 1,823,427 + +0.5 1,823,428 - +1.4 1,823,431 - -1.9 1,823,441 + -0.0 1,823,441 + +2.1 1,823,442 - -0.0 1,823,442 - -0.0 1,823,442 - -2.3 1,823,442 - +2.8
Or see this region's nucleotide sequence