Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1613 and Psyr_1614 are separated by 120 nucleotides Psyr_1614 and Psyr_1615 are separated by 32 nucleotides
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Psyr_1614: Psyr_1614 - lipid A biosynthesis acyltransferase, at 1,816,749 to 1,817,681
_1614
Psyr_1615: Psyr_1615 - Patatin, at 1,817,714 to 1,818,898
_1615
Position (kb)
1816
1817
1818 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1815.770 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.088 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.594 kb on - strand at 1816.594 kb on - strand at 1816.635 kb on + strand at 1816.635 kb on + strand at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.673 kb on + strand at 1816.714 kb on + strand at 1816.714 kb on + strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.891 kb on - strand, within Psyr_1614 at 1816.891 kb on - strand, within Psyr_1614 at 1817.154 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.203 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.341 kb on - strand, within Psyr_1614 at 1817.611 kb on - strand at 1817.662 kb on + strand at 1817.662 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.676 kb on - strand at 1817.676 kb on - strand at 1817.679 kb on + strand at 1817.679 kb on + strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.808 kb on - strand at 1817.997 kb on + strand, within Psyr_1615 at 1818.259 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.469 kb on + strand, within Psyr_1615 at 1818.470 kb on - strand, within Psyr_1615 at 1818.470 kb on - strand, within Psyr_1615 at 1818.556 kb on + strand, within Psyr_1615 at 1818.562 kb on + strand, within Psyr_1615
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,815,770 - -0.2 1,815,791 - +1.6 1,815,791 - -0.4 1,815,791 - -4.1 1,815,791 - +2.3 1,815,791 - -2.1 1,815,982 + Psyr_1613 0.13 -3.3 1,815,982 + Psyr_1613 0.13 -2.3 1,815,982 + Psyr_1613 0.13 +0.0 1,815,983 - Psyr_1613 0.13 -3.6 1,815,983 - Psyr_1613 0.13 -2.3 1,816,088 - Psyr_1613 0.27 +0.0 1,816,168 + Psyr_1613 0.38 -4.3 1,816,168 + Psyr_1613 0.38 +0.0 1,816,168 + Psyr_1613 0.38 -5.0 1,816,168 + Psyr_1613 0.38 +1.0 1,816,169 - Psyr_1613 0.38 -3.9 1,816,169 - Psyr_1613 0.38 -0.7 1,816,169 - Psyr_1613 0.38 -3.2 1,816,473 - Psyr_1613 0.79 -3.4 1,816,594 - +0.0 1,816,594 - +0.0 1,816,635 + +0.0 1,816,635 + +0.0 1,816,636 - +0.0 1,816,637 + -2.8 1,816,638 - -0.1 1,816,638 - +2.0 1,816,638 - +0.3 1,816,638 - +0.1 1,816,638 - +0.0 1,816,638 - -0.9 1,816,638 - -1.3 1,816,638 - -2.1 1,816,638 - +1.0 1,816,672 - -0.7 1,816,672 - +4.3 1,816,672 - +3.0 1,816,672 - -2.4 1,816,672 - -1.8 1,816,672 - +1.0 1,816,673 + +0.0 1,816,714 + +0.0 1,816,714 + +2.8 1,816,715 - -2.2 1,816,715 - +0.6 1,816,715 - +2.0 1,816,715 - +1.0 1,816,891 - Psyr_1614 0.15 +0.0 1,816,891 - Psyr_1614 0.15 +0.0 1,817,154 + Psyr_1614 0.43 -5.5 1,817,202 + Psyr_1614 0.49 +1.6 1,817,202 + Psyr_1614 0.49 -3.6 1,817,203 - Psyr_1614 0.49 -2.8 1,817,315 - Psyr_1614 0.61 -4.2 1,817,341 - Psyr_1614 0.63 -5.5 1,817,611 - +0.0 1,817,662 + +1.6 1,817,662 + -4.0 1,817,664 + -1.6 1,817,664 + +2.3 1,817,664 + -3.9 1,817,664 + -3.1 1,817,664 + -1.0 1,817,665 - -1.3 1,817,665 - +3.9 1,817,665 - +3.2 1,817,665 - -3.0 1,817,665 - -2.2 1,817,665 - -4.8 1,817,665 - +2.0 1,817,675 + -3.3 1,817,675 + -1.0 1,817,675 + -1.9 1,817,676 - -3.0 1,817,676 - +2.0 1,817,679 + +1.0 1,817,679 + -0.7 1,817,680 - -0.1 1,817,680 - +1.6 1,817,680 - +0.4 1,817,680 - -2.2 1,817,680 - -1.0 1,817,680 - +1.6 1,817,711 + -3.8 1,817,711 + +2.3 1,817,711 + -0.3 1,817,711 + -1.1 1,817,711 + -0.6 1,817,711 + +1.0 1,817,711 + +1.0 1,817,712 - -1.3 1,817,712 - +2.6 1,817,712 - +2.0 1,817,712 - -0.5 1,817,712 - -2.1 1,817,712 - +2.0 1,817,712 - +3.3 1,817,712 - -0.9 1,817,712 - +0.2 1,817,712 - +2.3 1,817,808 - -0.9 1,817,997 + Psyr_1615 0.24 +2.3 1,818,259 - Psyr_1615 0.46 +4.6 1,818,363 - Psyr_1615 0.55 +2.6 1,818,363 - Psyr_1615 0.55 +2.3 1,818,469 + Psyr_1615 0.64 +2.0 1,818,470 - Psyr_1615 0.64 +2.0 1,818,470 - Psyr_1615 0.64 -0.7 1,818,556 + Psyr_1615 0.71 -1.4 1,818,562 + Psyr_1615 0.72 +2.6
Or see this region's nucleotide sequence