Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1586 and Psyr_1587 are separated by 499 nucleotides Psyr_1587 and Psyr_1588 are separated by 96 nucleotides
Psyr_1586: Psyr_1586 - Phospholipid/glycerol acyltransferase:Phospholipid/glycerol acyltransferase, at 1,783,706 to 1,785,580
_1586
Psyr_1587: Psyr_1587 - Bile acid:sodium symporter, at 1,786,080 to 1,787,027
_1587
Psyr_1588: Psyr_1588 - Putative exonuclease, RdgC, at 1,787,124 to 1,788,044
_1588
Position (kb)
1786
1787
1788 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1785.158 kb on + strand, within Psyr_1586 at 1785.159 kb on - strand, within Psyr_1586 at 1785.159 kb on - strand, within Psyr_1586 at 1785.159 kb on - strand, within Psyr_1586 at 1785.159 kb on - strand, within Psyr_1586 at 1785.159 kb on - strand, within Psyr_1586 at 1785.161 kb on + strand, within Psyr_1586 at 1785.161 kb on + strand, within Psyr_1586 at 1785.161 kb on + strand, within Psyr_1586 at 1785.162 kb on - strand, within Psyr_1586 at 1785.162 kb on - strand, within Psyr_1586 at 1785.171 kb on - strand, within Psyr_1586 at 1785.171 kb on - strand, within Psyr_1586 at 1785.187 kb on + strand, within Psyr_1586 at 1785.187 kb on + strand, within Psyr_1586 at 1785.187 kb on + strand, within Psyr_1586 at 1785.188 kb on - strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.251 kb on + strand, within Psyr_1586 at 1785.252 kb on - strand, within Psyr_1586 at 1785.252 kb on - strand, within Psyr_1586 at 1785.281 kb on + strand, within Psyr_1586 at 1785.540 kb on + strand at 1785.541 kb on - strand at 1785.541 kb on - strand at 1785.594 kb on + strand at 1785.594 kb on + strand at 1785.653 kb on + strand at 1785.653 kb on + strand at 1785.653 kb on + strand at 1785.654 kb on - strand at 1785.654 kb on - strand at 1785.654 kb on - strand at 1785.880 kb on - strand at 1785.880 kb on - strand at 1785.880 kb on - strand at 1785.898 kb on + strand at 1785.898 kb on + strand at 1785.899 kb on - strand at 1785.899 kb on - strand at 1785.899 kb on - strand at 1785.899 kb on - strand at 1785.899 kb on - strand at 1786.068 kb on - strand at 1786.068 kb on - strand at 1786.094 kb on + strand at 1786.094 kb on + strand at 1786.094 kb on + strand at 1786.095 kb on - strand at 1786.095 kb on - strand at 1786.123 kb on + strand at 1786.123 kb on + strand at 1786.123 kb on + strand at 1786.123 kb on + strand at 1786.124 kb on - strand at 1786.148 kb on + strand at 1786.148 kb on + strand at 1786.149 kb on - strand at 1786.307 kb on - strand at 1786.421 kb on + strand, within Psyr_1587 at 1786.422 kb on - strand, within Psyr_1587 at 1786.422 kb on - strand, within Psyr_1587 at 1786.422 kb on - strand, within Psyr_1587 at 1786.459 kb on + strand, within Psyr_1587 at 1786.459 kb on + strand, within Psyr_1587 at 1786.460 kb on - strand, within Psyr_1587 at 1786.545 kb on - strand, within Psyr_1587 at 1786.642 kb on - strand, within Psyr_1587 at 1786.920 kb on - strand, within Psyr_1587 at 1787.040 kb on - strand at 1787.040 kb on - strand at 1787.746 kb on + strand, within Psyr_1588 at 1787.746 kb on + strand, within Psyr_1588 at 1787.874 kb on + strand, within Psyr_1588 at 1787.920 kb on + strand, within Psyr_1588 at 1787.920 kb on + strand, within Psyr_1588 at 1787.920 kb on + strand, within Psyr_1588 at 1787.920 kb on + strand, within Psyr_1588 at 1787.921 kb on - strand, within Psyr_1588 at 1787.921 kb on - strand, within Psyr_1588 at 1787.928 kb on + strand, within Psyr_1588 at 1787.929 kb on - strand, within Psyr_1588
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,785,158 + Psyr_1586 0.77 -1.3 1,785,159 - Psyr_1586 0.77 -1.8 1,785,159 - Psyr_1586 0.77 +2.3 1,785,159 - Psyr_1586 0.77 -0.5 1,785,159 - Psyr_1586 0.77 -1.2 1,785,159 - Psyr_1586 0.77 -1.1 1,785,161 + Psyr_1586 0.78 +3.0 1,785,161 + Psyr_1586 0.78 +0.5 1,785,161 + Psyr_1586 0.78 -1.4 1,785,162 - Psyr_1586 0.78 -1.9 1,785,162 - Psyr_1586 0.78 -1.6 1,785,171 - Psyr_1586 0.78 -2.2 1,785,171 - Psyr_1586 0.78 -1.3 1,785,187 + Psyr_1586 0.79 -2.0 1,785,187 + Psyr_1586 0.79 +0.1 1,785,187 + Psyr_1586 0.79 -0.9 1,785,188 - Psyr_1586 0.79 +0.4 1,785,251 + Psyr_1586 0.82 -0.5 1,785,251 + Psyr_1586 0.82 +0.0 1,785,251 + Psyr_1586 0.82 -1.3 1,785,251 + Psyr_1586 0.82 +2.3 1,785,251 + Psyr_1586 0.82 -4.0 1,785,251 + Psyr_1586 0.82 +0.4 1,785,252 - Psyr_1586 0.82 +2.0 1,785,252 - Psyr_1586 0.82 -0.8 1,785,281 + Psyr_1586 0.84 -2.0 1,785,540 + +0.4 1,785,541 - -1.3 1,785,541 - -1.2 1,785,594 + -0.6 1,785,594 + +3.8 1,785,653 + +2.0 1,785,653 + +2.3 1,785,653 + +0.0 1,785,654 - +3.9 1,785,654 - -0.4 1,785,654 - +3.8 1,785,880 - -0.1 1,785,880 - +0.2 1,785,880 - -0.1 1,785,898 + +2.0 1,785,898 + +3.0 1,785,899 - +0.0 1,785,899 - -1.6 1,785,899 - -0.2 1,785,899 - +0.3 1,785,899 - -1.9 1,786,068 - -0.7 1,786,068 - +2.8 1,786,094 + +1.6 1,786,094 + -0.8 1,786,094 + -2.8 1,786,095 - -1.5 1,786,095 - +1.6 1,786,123 + -2.2 1,786,123 + -1.6 1,786,123 + +3.0 1,786,123 + +2.0 1,786,124 - +2.8 1,786,148 + +2.8 1,786,148 + -1.6 1,786,149 - +1.6 1,786,307 - -3.6 1,786,421 + Psyr_1587 0.36 +1.0 1,786,422 - Psyr_1587 0.36 -0.4 1,786,422 - Psyr_1587 0.36 +2.0 1,786,422 - Psyr_1587 0.36 -4.7 1,786,459 + Psyr_1587 0.40 +3.6 1,786,459 + Psyr_1587 0.40 +2.3 1,786,460 - Psyr_1587 0.40 -3.1 1,786,545 - Psyr_1587 0.49 +0.9 1,786,642 - Psyr_1587 0.59 +1.1 1,786,920 - Psyr_1587 0.89 +3.3 1,787,040 - +3.0 1,787,040 - +3.8 1,787,746 + Psyr_1588 0.68 -0.5 1,787,746 + Psyr_1588 0.68 -2.3 1,787,874 + Psyr_1588 0.81 -1.0 1,787,920 + Psyr_1588 0.86 -0.4 1,787,920 + Psyr_1588 0.86 -1.7 1,787,920 + Psyr_1588 0.86 +3.6 1,787,920 + Psyr_1588 0.86 +1.0 1,787,921 - Psyr_1588 0.87 +1.6 1,787,921 - Psyr_1588 0.87 +3.2 1,787,928 + Psyr_1588 0.87 +2.0 1,787,929 - Psyr_1588 0.87 +0.0
Or see this region's nucleotide sequence