Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1544

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1543 and Psyr_1544 are separated by 140 nucleotidesPsyr_1544 and Psyr_1545 are separated by 34 nucleotides Psyr_1543: Psyr_1543 - Peptidase M48, Ste24p, at 1,736,975 to 1,738,408 _1543 Psyr_1544: Psyr_1544 - SirA-like protein, at 1,738,549 to 1,738,800 _1544 Psyr_1545: Psyr_1545 - Protein of unknown function UPF0118, at 1,738,835 to 1,739,905 _1545 Position (kb) 1738 1739Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3 4at 1737.579 kb on + strand, within Psyr_1543at 1737.580 kb on - strand, within Psyr_1543at 1737.580 kb on - strand, within Psyr_1543at 1737.580 kb on - strand, within Psyr_1543at 1737.613 kb on - strand, within Psyr_1543at 1737.613 kb on - strand, within Psyr_1543at 1737.613 kb on - strand, within Psyr_1543at 1738.212 kb on + strand, within Psyr_1543at 1738.212 kb on + strand, within Psyr_1543at 1738.213 kb on - strand, within Psyr_1543at 1738.213 kb on - strand, within Psyr_1543at 1738.436 kb on + strandat 1738.436 kb on + strandat 1738.437 kb on - strandat 1738.437 kb on - strandat 1738.441 kb on + strandat 1738.441 kb on + strandat 1738.441 kb on + strandat 1738.441 kb on + strandat 1738.441 kb on + strandat 1738.441 kb on + strandat 1738.442 kb on - strandat 1738.475 kb on + strandat 1738.475 kb on + strandat 1738.475 kb on + strandat 1738.475 kb on + strandat 1738.475 kb on + strandat 1738.476 kb on - strandat 1738.476 kb on - strandat 1738.476 kb on - strandat 1738.544 kb on - strandat 1738.563 kb on - strandat 1738.563 kb on - strandat 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.679 kb on + strand, within Psyr_1544at 1738.680 kb on - strand, within Psyr_1544at 1738.680 kb on - strand, within Psyr_1544at 1738.680 kb on - strand, within Psyr_1544at 1738.774 kb on + strand, within Psyr_1544at 1738.774 kb on + strand, within Psyr_1544at 1738.774 kb on + strand, within Psyr_1544at 1738.775 kb on - strandat 1738.775 kb on - strandat 1738.868 kb on + strandat 1738.868 kb on + strandat 1738.868 kb on + strandat 1738.868 kb on + strandat 1738.869 kb on - strandat 1738.869 kb on - strandat 1738.869 kb on - strandat 1738.869 kb on - strandat 1739.073 kb on + strand, within Psyr_1545at 1739.074 kb on - strand, within Psyr_1545at 1739.074 kb on - strand, within Psyr_1545at 1739.121 kb on + strand, within Psyr_1545at 1739.150 kb on - strand, within Psyr_1545at 1739.178 kb on + strand, within Psyr_1545at 1739.178 kb on + strand, within Psyr_1545at 1739.178 kb on + strand, within Psyr_1545at 1739.179 kb on - strand, within Psyr_1545at 1739.179 kb on - strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.327 kb on + strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strandat 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.328 kb on - strand, within Psyr_1545at 1739.403 kb on - strand, within Psyr_1545at 1739.403 kb on - strand, within Psyr_1545at 1739.403 kb on - strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.468 kb on + strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.469 kb on - strand, within Psyr_1545at 1739.470 kb on + strand, within Psyr_1545at 1739.470 kb on + strand, within Psyr_1545at 1739.470 kb on + strand, within Psyr_1545at 1739.470 kb on + strand, within Psyr_1545at 1739.470 kb on + strand, within Psyr_1545at 1739.471 kb on - strand, within Psyr_1545at 1739.471 kb on - strand, within Psyr_1545at 1739.471 kb on - strand, within Psyr_1545at 1739.471 kb on - strand, within Psyr_1545at 1739.498 kb on - strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.516 kb on + strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.517 kb on - strand, within Psyr_1545at 1739.546 kb on + strand, within Psyr_1545at 1739.547 kb on - strand, within Psyr_1545at 1739.547 kb on - strand, within Psyr_1545at 1739.547 kb on - strand, within Psyr_1545at 1739.594 kb on + strand, within Psyr_1545at 1739.594 kb on + strand, within Psyr_1545at 1739.594 kb on + strand, within Psyr_1545at 1739.595 kb on - strand, within Psyr_1545at 1739.672 kb on + strand, within Psyr_1545at 1739.673 kb on - strand, within Psyr_1545

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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1,737,579 + Psyr_1543 0.42 -0.6
1,737,580 - Psyr_1543 0.42 -2.9
1,737,580 - Psyr_1543 0.42 -2.1
1,737,580 - Psyr_1543 0.42 +0.0
1,737,613 - Psyr_1543 0.44 -3.4
1,737,613 - Psyr_1543 0.44 +0.0
1,737,613 - Psyr_1543 0.44 -1.7
1,738,212 + Psyr_1543 0.86 -1.0
1,738,212 + Psyr_1543 0.86 +2.0
1,738,213 - Psyr_1543 0.86 -1.2
1,738,213 - Psyr_1543 0.86 -5.7
1,738,436 + -0.6
1,738,436 + -3.0
1,738,437 - -0.2
1,738,437 - +0.6
1,738,441 + -2.4
1,738,441 + -2.7
1,738,441 + -1.4
1,738,441 + +3.5
1,738,441 + -1.5
1,738,441 + -2.7
1,738,442 - +1.0
1,738,475 + -1.3
1,738,475 + +1.0
1,738,475 + +1.0
1,738,475 + -0.8
1,738,475 + +3.2
1,738,476 - -0.9
1,738,476 - -0.8
1,738,476 - +1.6
1,738,544 - -5.0
1,738,563 - +0.0
1,738,563 - +1.0
1,738,679 + Psyr_1544 0.52 -4.5
1,738,679 + Psyr_1544 0.52 +1.0
1,738,679 + Psyr_1544 0.52 +1.0
1,738,679 + Psyr_1544 0.52 -4.1
1,738,679 + Psyr_1544 0.52 -2.0
1,738,679 + Psyr_1544 0.52 -3.8
1,738,679 + Psyr_1544 0.52 -4.6
1,738,679 + Psyr_1544 0.52 -4.2
1,738,680 - Psyr_1544 0.52 -4.8
1,738,680 - Psyr_1544 0.52 -4.4
1,738,680 - Psyr_1544 0.52 -5.9
1,738,774 + Psyr_1544 0.89 -3.4
1,738,774 + Psyr_1544 0.89 +0.0
1,738,774 + Psyr_1544 0.89 -4.5
1,738,775 - -3.4
1,738,775 - -1.4
1,738,868 + +3.6
1,738,868 + -1.3
1,738,868 + -2.2
1,738,868 + +2.4
1,738,869 - +2.6
1,738,869 - -1.2
1,738,869 - +2.4
1,738,869 - +2.4
1,739,073 + Psyr_1545 0.22 +0.0
1,739,074 - Psyr_1545 0.22 +3.2
1,739,074 - Psyr_1545 0.22 +2.8
1,739,121 + Psyr_1545 0.27 +1.7
1,739,150 - Psyr_1545 0.29 +0.5
1,739,178 + Psyr_1545 0.32 -0.2
1,739,178 + Psyr_1545 0.32 +2.0
1,739,178 + Psyr_1545 0.32 +1.6
1,739,179 - Psyr_1545 0.32 +0.9
1,739,179 - Psyr_1545 0.32 +2.6
1,739,327 + Psyr_1545 0.46 +0.0
1,739,327 + Psyr_1545 0.46 +3.7
1,739,327 + Psyr_1545 0.46 -2.2
1,739,327 + Psyr_1545 0.46 +3.9
1,739,327 + Psyr_1545 0.46 -0.6
1,739,327 + Psyr_1545 0.46 -1.6
1,739,327 + Psyr_1545 0.46 -0.3
1,739,327 + Psyr_1545 0.46 +4.0
1,739,328 - Psyr_1545 0.46 -1.7
1,739,328 - Psyr_1545 0.46 -5.3
1,739,328 - Psyr_1545 0.46 +2.6
1,739,328 - Psyr_1545 0.46 -1.5
1,739,328 - +1.0
1,739,328 - Psyr_1545 0.46 -2.3
1,739,328 - Psyr_1545 0.46 +2.4
1,739,328 - Psyr_1545 0.46 +3.5
1,739,328 - Psyr_1545 0.46 -3.7
1,739,328 - Psyr_1545 0.46 +3.4
1,739,328 - Psyr_1545 0.46 +0.4
1,739,403 - Psyr_1545 0.53 -0.9
1,739,403 - Psyr_1545 0.53 -0.5
1,739,403 - Psyr_1545 0.53 -1.2
1,739,468 + Psyr_1545 0.59 -0.1
1,739,468 + Psyr_1545 0.59 +3.6
1,739,468 + Psyr_1545 0.59 -0.5
1,739,468 + Psyr_1545 0.59 +3.2
1,739,468 + Psyr_1545 0.59 -1.3
1,739,468 + Psyr_1545 0.59 +0.4
1,739,468 + Psyr_1545 0.59 +0.6
1,739,468 + Psyr_1545 0.59 +3.4
1,739,468 + Psyr_1545 0.59 +2.0
1,739,468 + Psyr_1545 0.59 -0.2
1,739,468 + Psyr_1545 0.59 +2.0
1,739,468 + Psyr_1545 0.59 +1.0
1,739,468 + Psyr_1545 0.59 -1.4
1,739,468 + Psyr_1545 0.59 +3.0
1,739,468 + Psyr_1545 0.59 +1.6
1,739,468 + Psyr_1545 0.59 -1.3
1,739,468 + Psyr_1545 0.59 +0.0
1,739,469 - Psyr_1545 0.59 +2.0
1,739,469 - Psyr_1545 0.59 -1.9
1,739,469 - Psyr_1545 0.59 +3.5
1,739,469 - Psyr_1545 0.59 +1.0
1,739,469 - Psyr_1545 0.59 -2.1
1,739,469 - Psyr_1545 0.59 +1.0
1,739,469 - Psyr_1545 0.59 -1.6
1,739,469 - Psyr_1545 0.59 +0.0
1,739,469 - Psyr_1545 0.59 +0.4
1,739,469 - Psyr_1545 0.59 -2.4
1,739,469 - Psyr_1545 0.59 -1.3
1,739,469 - Psyr_1545 0.59 +4.7
1,739,469 - Psyr_1545 0.59 -1.3
1,739,469 - Psyr_1545 0.59 +3.0
1,739,470 + Psyr_1545 0.59 +3.2
1,739,470 + Psyr_1545 0.59 +3.2
1,739,470 + Psyr_1545 0.59 -1.4
1,739,470 + Psyr_1545 0.59 +2.8
1,739,470 + Psyr_1545 0.59 +0.4
1,739,471 - Psyr_1545 0.59 +2.4
1,739,471 - Psyr_1545 0.59 +0.0
1,739,471 - Psyr_1545 0.59 +2.0
1,739,471 - Psyr_1545 0.59 +2.0
1,739,498 - Psyr_1545 0.62 -0.3
1,739,516 + Psyr_1545 0.64 -0.6
1,739,516 + Psyr_1545 0.64 -0.6
1,739,516 + Psyr_1545 0.64 +3.0
1,739,516 + Psyr_1545 0.64 -0.9
1,739,516 + Psyr_1545 0.64 -0.5
1,739,516 + Psyr_1545 0.64 +4.3
1,739,516 + Psyr_1545 0.64 -2.1
1,739,517 - Psyr_1545 0.64 -1.0
1,739,517 - Psyr_1545 0.64 +2.4
1,739,517 - Psyr_1545 0.64 -0.4
1,739,517 - Psyr_1545 0.64 +1.6
1,739,517 - Psyr_1545 0.64 +2.0
1,739,517 - Psyr_1545 0.64 -0.5
1,739,546 + Psyr_1545 0.66 -0.8
1,739,547 - Psyr_1545 0.66 -1.4
1,739,547 - Psyr_1545 0.66 +2.8
1,739,547 - Psyr_1545 0.66 +0.0
1,739,594 + Psyr_1545 0.71 +2.6
1,739,594 + Psyr_1545 0.71 +3.4
1,739,594 + Psyr_1545 0.71 +0.4
1,739,595 - Psyr_1545 0.71 -1.9
1,739,672 + Psyr_1545 0.78 -0.2
1,739,673 - Psyr_1545 0.78 -3.4

Or see this region's nucleotide sequence