Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1513 and Psyr_1514 overlap by 11 nucleotides Psyr_1514 and Psyr_1515 overlap by 4 nucleotides Psyr_1515 and Psyr_1516 overlap by 11 nucleotides
Psyr_1513: Psyr_1513 - type II secretion system protein, at 1,699,012 to 1,700,103
_1513
Psyr_1514: Psyr_1514 - type II secretion system protein E, at 1,700,093 to 1,701,724
_1514
Psyr_1515: Psyr_1515 - hypothetical protein, at 1,701,721 to 1,702,323
_1515
Psyr_1516: Psyr_1516 - hypothetical protein, at 1,702,313 to 1,703,671
_1516
Position (kb) 1700
1701
1702 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 1699.147 kb on + strand, within Psyr_1513 at 1699.148 kb on - strand, within Psyr_1513 at 1699.264 kb on + strand, within Psyr_1513 at 1699.264 kb on + strand, within Psyr_1513 at 1699.264 kb on + strand, within Psyr_1513 at 1699.265 kb on - strand, within Psyr_1513 at 1699.265 kb on - strand, within Psyr_1513 at 1699.265 kb on - strand, within Psyr_1513 at 1699.265 kb on - strand, within Psyr_1513 at 1699.265 kb on - strand, within Psyr_1513 at 1699.351 kb on + strand, within Psyr_1513 at 1699.439 kb on + strand, within Psyr_1513 at 1699.439 kb on + strand, within Psyr_1513 at 1699.439 kb on + strand at 1699.439 kb on + strand, within Psyr_1513 at 1699.440 kb on - strand, within Psyr_1513 at 1699.440 kb on - strand, within Psyr_1513 at 1699.440 kb on - strand, within Psyr_1513 at 1699.440 kb on - strand, within Psyr_1513 at 1699.494 kb on - strand, within Psyr_1513 at 1699.560 kb on + strand, within Psyr_1513 at 1699.561 kb on - strand, within Psyr_1513 at 1699.561 kb on - strand, within Psyr_1513 at 1699.660 kb on + strand, within Psyr_1513 at 1699.661 kb on - strand, within Psyr_1513 at 1699.673 kb on + strand, within Psyr_1513 at 1699.674 kb on - strand, within Psyr_1513 at 1699.674 kb on - strand, within Psyr_1513 at 1699.674 kb on - strand, within Psyr_1513 at 1699.674 kb on - strand, within Psyr_1513 at 1699.842 kb on + strand, within Psyr_1513 at 1699.888 kb on + strand, within Psyr_1513 at 1699.888 kb on + strand, within Psyr_1513 at 1699.895 kb on + strand, within Psyr_1513 at 1699.895 kb on + strand, within Psyr_1513 at 1699.943 kb on + strand, within Psyr_1513 at 1699.943 kb on + strand, within Psyr_1513 at 1699.943 kb on + strand at 1699.943 kb on + strand, within Psyr_1513 at 1699.944 kb on - strand, within Psyr_1513 at 1699.944 kb on - strand, within Psyr_1513 at 1699.944 kb on - strand, within Psyr_1513 at 1699.944 kb on - strand, within Psyr_1513 at 1699.944 kb on - strand, within Psyr_1513 at 1699.956 kb on + strand, within Psyr_1513 at 1699.956 kb on + strand, within Psyr_1513 at 1700.054 kb on + strand at 1700.054 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.065 kb on + strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.066 kb on - strand at 1700.094 kb on + strand at 1700.095 kb on - strand at 1700.095 kb on - strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.139 kb on + strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.140 kb on - strand at 1700.200 kb on + strand at 1700.200 kb on + strand at 1700.200 kb on + strand at 1700.201 kb on - strand at 1700.201 kb on - strand at 1700.201 kb on - strand at 1700.204 kb on + strand at 1700.204 kb on + strand at 1700.204 kb on + strand at 1700.205 kb on - strand at 1700.205 kb on - strand at 1700.366 kb on + strand, within Psyr_1514 at 1700.366 kb on + strand, within Psyr_1514 at 1700.449 kb on + strand, within Psyr_1514 at 1700.516 kb on - strand, within Psyr_1514 at 1700.554 kb on + strand, within Psyr_1514 at 1700.554 kb on + strand, within Psyr_1514 at 1700.554 kb on + strand, within Psyr_1514 at 1700.555 kb on - strand, within Psyr_1514 at 1700.555 kb on - strand, within Psyr_1514 at 1700.555 kb on - strand, within Psyr_1514 at 1700.592 kb on - strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.688 kb on + strand, within Psyr_1514 at 1700.689 kb on - strand, within Psyr_1514 at 1700.689 kb on - strand, within Psyr_1514 at 1700.689 kb on - strand, within Psyr_1514 at 1700.696 kb on + strand, within Psyr_1514 at 1700.696 kb on + strand, within Psyr_1514 at 1700.697 kb on - strand, within Psyr_1514 at 1700.697 kb on - strand, within Psyr_1514 at 1700.697 kb on - strand, within Psyr_1514 at 1700.725 kb on + strand, within Psyr_1514 at 1700.725 kb on + strand, within Psyr_1514 at 1700.812 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.901 kb on - strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.001 kb on - strand, within Psyr_1514 at 1701.020 kb on + strand, within Psyr_1514 at 1701.020 kb on + strand, within Psyr_1514 at 1701.051 kb on - strand, within Psyr_1514 at 1701.126 kb on + strand, within Psyr_1514 at 1701.127 kb on - strand, within Psyr_1514 at 1701.127 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.246 kb on + strand, within Psyr_1514 at 1701.246 kb on + strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.314 kb on - strand, within Psyr_1514 at 1701.314 kb on - strand, within Psyr_1514 at 1701.340 kb on + strand, within Psyr_1514 at 1701.347 kb on + strand, within Psyr_1514 at 1701.347 kb on + strand, within Psyr_1514 at 1701.348 kb on - strand, within Psyr_1514 at 1701.348 kb on - strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.454 kb on + strand, within Psyr_1514 at 1701.454 kb on + strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.534 kb on - strand, within Psyr_1514 at 1701.856 kb on + strand, within Psyr_1515 at 1701.856 kb on + strand, within Psyr_1515 at 1701.857 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.942 kb on + strand, within Psyr_1515 at 1701.982 kb on + strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1702.073 kb on + strand, within Psyr_1515 at 1702.073 kb on + strand, within Psyr_1515 at 1702.086 kb on + strand, within Psyr_1515 at 1702.282 kb on + strand at 1702.282 kb on + strand at 1702.282 kb on + strand at 1702.283 kb on - strand at 1702.283 kb on - strand at 1702.283 kb on - strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.334 kb on + strand at 1702.335 kb on - strand at 1702.349 kb on + strand at 1702.538 kb on + strand, within Psyr_1516 at 1702.539 kb on - strand, within Psyr_1516 at 1702.696 kb on + strand, within Psyr_1516 at 1702.707 kb on + strand, within Psyr_1516
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,699,147 + Psyr_1513 0.12 -1.0 1,699,148 - Psyr_1513 0.12 -0.3 1,699,264 + Psyr_1513 0.23 +3.2 1,699,264 + Psyr_1513 0.23 +0.0 1,699,264 + Psyr_1513 0.23 +1.4 1,699,265 - Psyr_1513 0.23 +2.8 1,699,265 - Psyr_1513 0.23 +1.0 1,699,265 - Psyr_1513 0.23 +1.6 1,699,265 - Psyr_1513 0.23 -0.4 1,699,265 - Psyr_1513 0.23 +0.0 1,699,351 + Psyr_1513 0.31 +2.0 1,699,439 + Psyr_1513 0.39 +2.0 1,699,439 + Psyr_1513 0.39 -1.6 1,699,439 + +1.6 1,699,439 + Psyr_1513 0.39 -0.6 1,699,440 - Psyr_1513 0.39 +2.4 1,699,440 - Psyr_1513 0.39 +2.8 1,699,440 - Psyr_1513 0.39 -2.0 1,699,440 - Psyr_1513 0.39 +2.4 1,699,494 - Psyr_1513 0.44 +0.3 1,699,560 + Psyr_1513 0.50 -0.6 1,699,561 - Psyr_1513 0.50 +0.4 1,699,561 - Psyr_1513 0.50 +2.0 1,699,660 + Psyr_1513 0.59 +1.6 1,699,661 - Psyr_1513 0.59 -1.1 1,699,673 + Psyr_1513 0.61 +1.6 1,699,674 - Psyr_1513 0.61 -2.7 1,699,674 - Psyr_1513 0.61 -0.3 1,699,674 - Psyr_1513 0.61 +3.6 1,699,674 - Psyr_1513 0.61 +3.2 1,699,842 + Psyr_1513 0.76 +3.7 1,699,888 + Psyr_1513 0.80 -1.8 1,699,888 + Psyr_1513 0.80 -1.5 1,699,895 + Psyr_1513 0.81 +0.7 1,699,895 + Psyr_1513 0.81 -4.9 1,699,943 + Psyr_1513 0.85 -1.0 1,699,943 + Psyr_1513 0.85 +1.6 1,699,943 + +0.0 1,699,943 + Psyr_1513 0.85 -2.6 1,699,944 - Psyr_1513 0.85 +2.6 1,699,944 - Psyr_1513 0.85 -2.1 1,699,944 - Psyr_1513 0.85 +0.6 1,699,944 - Psyr_1513 0.85 +2.4 1,699,944 - Psyr_1513 0.85 -3.6 1,699,956 + Psyr_1513 0.86 -3.8 1,699,956 + Psyr_1513 0.86 -3.0 1,700,054 + +3.4 1,700,054 + +0.0 1,700,065 + -1.6 1,700,065 + -4.8 1,700,065 + -3.0 1,700,065 + -0.9 1,700,065 + -3.2 1,700,065 + -3.8 1,700,065 + +0.0 1,700,065 + +0.0 1,700,066 - +2.6 1,700,066 - -1.5 1,700,066 - +2.6 1,700,066 - +4.2 1,700,066 - +2.4 1,700,066 - -0.5 1,700,066 - +3.2 1,700,066 - +1.8 1,700,094 + +3.4 1,700,095 - +2.4 1,700,095 - -3.9 1,700,139 + -1.8 1,700,139 + -0.1 1,700,139 + +0.0 1,700,139 + -3.3 1,700,139 + -0.2 1,700,139 + -3.6 1,700,139 + +0.0 1,700,139 + -3.1 1,700,139 + +1.6 1,700,139 + -1.8 1,700,139 + -3.4 1,700,139 + -1.6 1,700,139 + +2.4 1,700,140 - -0.0 1,700,140 - -1.0 1,700,140 - -1.8 1,700,140 - +0.3 1,700,140 - +3.5 1,700,140 - +0.0 1,700,140 - +0.0 1,700,140 - -2.5 1,700,140 - -0.7 1,700,140 - -1.2 1,700,140 - -1.5 1,700,200 + -3.6 1,700,200 + +1.6 1,700,200 + -0.8 1,700,201 - -1.1 1,700,201 - +3.9 1,700,201 - -0.4 1,700,204 + +2.6 1,700,204 + -1.7 1,700,204 + +2.8 1,700,205 - -0.8 1,700,205 - -0.6 1,700,366 + Psyr_1514 0.17 -3.7 1,700,366 + Psyr_1514 0.17 +1.0 1,700,449 + Psyr_1514 0.22 -1.0 1,700,516 - Psyr_1514 0.26 +2.8 1,700,554 + Psyr_1514 0.28 +2.0 1,700,554 + Psyr_1514 0.28 -0.5 1,700,554 + Psyr_1514 0.28 -1.3 1,700,555 - Psyr_1514 0.28 -3.4 1,700,555 - Psyr_1514 0.28 +0.0 1,700,555 - Psyr_1514 0.28 +0.4 1,700,592 - Psyr_1514 0.31 +3.4 1,700,688 + Psyr_1514 0.36 +3.5 1,700,688 + Psyr_1514 0.36 +1.6 1,700,688 + Psyr_1514 0.36 -0.6 1,700,688 + Psyr_1514 0.36 -1.3 1,700,688 + Psyr_1514 0.36 -0.8 1,700,688 + Psyr_1514 0.36 -0.7 1,700,689 - Psyr_1514 0.37 -0.7 1,700,689 - Psyr_1514 0.37 +2.4 1,700,689 - Psyr_1514 0.37 +1.0 1,700,696 + Psyr_1514 0.37 +3.4 1,700,696 + Psyr_1514 0.37 -1.8 1,700,697 - Psyr_1514 0.37 +4.1 1,700,697 - Psyr_1514 0.37 +2.0 1,700,697 - Psyr_1514 0.37 +1.0 1,700,725 + Psyr_1514 0.39 -0.6 1,700,725 + Psyr_1514 0.39 +1.6 1,700,812 + Psyr_1514 0.44 +0.9 1,700,900 + Psyr_1514 0.49 -1.5 1,700,900 + Psyr_1514 0.49 -1.6 1,700,900 + Psyr_1514 0.49 -1.5 1,700,900 + Psyr_1514 0.49 -1.0 1,700,901 - Psyr_1514 0.50 -1.6 1,701,000 + Psyr_1514 0.56 +3.4 1,701,000 + Psyr_1514 0.56 +0.3 1,701,000 + Psyr_1514 0.56 +2.6 1,701,000 + Psyr_1514 0.56 +0.0 1,701,001 - Psyr_1514 0.56 +1.6 1,701,020 + Psyr_1514 0.57 +2.0 1,701,020 + Psyr_1514 0.57 -3.2 1,701,051 - Psyr_1514 0.59 +0.2 1,701,126 + Psyr_1514 0.63 +0.5 1,701,127 - Psyr_1514 0.63 -3.6 1,701,127 - Psyr_1514 0.63 -0.1 1,701,130 - Psyr_1514 0.64 +1.1 1,701,130 - Psyr_1514 0.64 +1.0 1,701,130 - Psyr_1514 0.64 +1.6 1,701,246 + Psyr_1514 0.71 +1.0 1,701,246 + Psyr_1514 0.71 +3.2 1,701,247 - Psyr_1514 0.71 +1.6 1,701,247 - Psyr_1514 0.71 -0.6 1,701,247 - Psyr_1514 0.71 +2.0 1,701,247 - Psyr_1514 0.71 +2.6 1,701,314 - Psyr_1514 0.75 +2.4 1,701,314 - Psyr_1514 0.75 -4.3 1,701,340 + Psyr_1514 0.76 +0.2 1,701,347 + Psyr_1514 0.77 -2.0 1,701,347 + Psyr_1514 0.77 +3.6 1,701,348 - Psyr_1514 0.77 -1.7 1,701,348 - Psyr_1514 0.77 -1.3 1,701,349 + Psyr_1514 0.77 -2.3 1,701,349 + Psyr_1514 0.77 -1.0 1,701,349 + Psyr_1514 0.77 +1.0 1,701,349 + Psyr_1514 0.77 -1.6 1,701,349 + Psyr_1514 0.77 +0.8 1,701,350 - Psyr_1514 0.77 -1.3 1,701,350 - Psyr_1514 0.77 +4.0 1,701,350 - Psyr_1514 0.77 -2.3 1,701,350 - Psyr_1514 0.77 +2.0 1,701,350 - Psyr_1514 0.77 +1.6 1,701,350 - Psyr_1514 0.77 -2.3 1,701,350 - Psyr_1514 0.77 +1.6 1,701,454 + Psyr_1514 0.83 +1.5 1,701,454 + Psyr_1514 0.83 +2.0 1,701,455 - Psyr_1514 0.83 -0.8 1,701,455 - Psyr_1514 0.83 +3.5 1,701,455 - Psyr_1514 0.83 +3.6 1,701,468 + Psyr_1514 0.84 -1.7 1,701,468 + Psyr_1514 0.84 +3.4 1,701,468 + Psyr_1514 0.84 -0.1 1,701,468 + Psyr_1514 0.84 -1.9 1,701,469 - Psyr_1514 0.84 -3.8 1,701,469 - Psyr_1514 0.84 +1.6 1,701,469 - Psyr_1514 0.84 -0.7 1,701,469 - Psyr_1514 0.84 +3.6 1,701,469 - Psyr_1514 0.84 -0.8 1,701,534 - Psyr_1514 0.88 +2.4 1,701,856 + Psyr_1515 0.22 +1.6 1,701,856 + Psyr_1515 0.22 -0.5 1,701,857 - Psyr_1515 0.23 +0.5 1,701,914 - Psyr_1515 0.32 +0.0 1,701,914 - Psyr_1515 0.32 +1.0 1,701,914 - Psyr_1515 0.32 +3.5 1,701,942 + Psyr_1515 0.37 -0.8 1,701,982 + Psyr_1515 0.43 -0.1 1,701,993 - Psyr_1515 0.45 -3.2 1,701,993 - Psyr_1515 0.45 +2.8 1,701,993 - Psyr_1515 0.45 +1.9 1,701,993 - Psyr_1515 0.45 +0.0 1,702,073 + Psyr_1515 0.58 +1.0 1,702,073 + Psyr_1515 0.58 +3.2 1,702,086 + Psyr_1515 0.61 -2.0 1,702,282 + +3.8 1,702,282 + +1.0 1,702,282 + -0.9 1,702,283 - +1.0 1,702,283 - +2.6 1,702,283 - +1.5 1,702,323 + -0.4 1,702,323 + -0.1 1,702,323 + +1.6 1,702,323 + +3.6 1,702,323 + -2.2 1,702,323 + -2.3 1,702,324 - -0.5 1,702,324 - -2.3 1,702,324 - +3.4 1,702,324 - +3.4 1,702,324 - +3.4 1,702,324 - -0.5 1,702,324 - -1.6 1,702,334 + -1.5 1,702,335 - +2.0 1,702,349 + -1.5 1,702,538 + Psyr_1516 0.17 +2.0 1,702,539 - Psyr_1516 0.17 -0.5 1,702,696 + Psyr_1516 0.28 -1.9 1,702,707 + Psyr_1516 0.29 +1.4
Or see this region's nucleotide sequence