Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1428 and Psyr_1429 are separated by 20 nucleotides Psyr_1429 and Psyr_1430 overlap by 4 nucleotides Psyr_1430 and Psyr_1431 are separated by 781 nucleotides
Psyr_1428: Psyr_1428 - conserved hypothetical protein, at 1,616,775 to 1,618,142
_1428
Psyr_1429: Psyr_1429 - Protein of unknown function DUF1527, at 1,618,163 to 1,619,101
_1429
Psyr_1430: Psyr_1430 - Protein of unknown function DUF1525, at 1,619,098 to 1,619,571
_1430
Psyr_1431: Psyr_1431 - hypothetical protein, at 1,620,353 to 1,620,643
_1431
Position (kb) 1619
1620 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1618.119 kb on - strand at 1618.119 kb on - strand at 1618.142 kb on + strand at 1618.142 kb on + strand at 1618.142 kb on + strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.177 kb on + strand at 1618.177 kb on + strand at 1618.286 kb on + strand, within Psyr_1429 at 1618.287 kb on - strand, within Psyr_1429 at 1618.330 kb on + strand, within Psyr_1429 at 1618.407 kb on + strand, within Psyr_1429 at 1618.408 kb on - strand, within Psyr_1429 at 1618.476 kb on + strand, within Psyr_1429 at 1618.476 kb on + strand, within Psyr_1429 at 1618.476 kb on + strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.513 kb on - strand, within Psyr_1429 at 1618.513 kb on - strand, within Psyr_1429 at 1618.585 kb on - strand, within Psyr_1429 at 1618.585 kb on - strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.700 kb on - strand, within Psyr_1429 at 1618.700 kb on - strand, within Psyr_1429 at 1618.728 kb on - strand, within Psyr_1429 at 1618.728 kb on - strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.760 kb on - strand, within Psyr_1429 at 1618.866 kb on + strand, within Psyr_1429 at 1618.866 kb on + strand, within Psyr_1429 at 1618.919 kb on + strand, within Psyr_1429 at 1618.919 kb on + strand, within Psyr_1429 at 1618.920 kb on - strand, within Psyr_1429 at 1618.920 kb on - strand, within Psyr_1429 at 1618.974 kb on + strand, within Psyr_1429 at 1618.974 kb on + strand, within Psyr_1429 at 1618.974 kb on + strand, within Psyr_1429 at 1618.975 kb on - strand, within Psyr_1429 at 1618.975 kb on - strand, within Psyr_1429 at 1618.975 kb on - strand, within Psyr_1429 at 1619.187 kb on + strand, within Psyr_1430 at 1619.187 kb on + strand, within Psyr_1430 at 1619.198 kb on + strand, within Psyr_1430 at 1619.198 kb on + strand, within Psyr_1430 at 1619.198 kb on + strand, within Psyr_1430 at 1619.199 kb on - strand, within Psyr_1430 at 1619.199 kb on - strand, within Psyr_1430 at 1619.199 kb on - strand, within Psyr_1430 at 1619.263 kb on + strand, within Psyr_1430 at 1619.263 kb on + strand, within Psyr_1430 at 1619.281 kb on + strand, within Psyr_1430 at 1619.282 kb on - strand, within Psyr_1430 at 1619.501 kb on - strand, within Psyr_1430 at 1619.501 kb on - strand, within Psyr_1430 at 1619.501 kb on - strand, within Psyr_1430 at 1619.572 kb on + strand at 1619.572 kb on + strand at 1619.572 kb on + strand at 1619.573 kb on - strand at 1619.579 kb on + strand at 1619.579 kb on + strand at 1619.718 kb on + strand at 1619.718 kb on + strand at 1619.719 kb on - strand at 1619.722 kb on + strand at 1619.746 kb on + strand at 1619.746 kb on + strand at 1619.753 kb on - strand at 1619.803 kb on + strand at 1619.804 kb on - strand at 1619.804 kb on - strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.913 kb on - strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.955 kb on - strand at 1619.955 kb on - strand at 1619.955 kb on - strand at 1619.955 kb on - strand at 1619.956 kb on + strand at 1619.986 kb on - strand at 1620.048 kb on + strand at 1620.065 kb on + strand at 1620.065 kb on + strand at 1620.066 kb on - strand at 1620.066 kb on - strand at 1620.066 kb on - strand at 1620.092 kb on + strand at 1620.101 kb on - strand at 1620.136 kb on + strand at 1620.136 kb on + strand at 1620.137 kb on - strand at 1620.137 kb on - strand at 1620.137 kb on - strand at 1620.137 kb on - strand at 1620.177 kb on + strand at 1620.178 kb on - strand at 1620.178 kb on - strand at 1620.178 kb on - strand at 1620.191 kb on + strand at 1620.191 kb on + strand at 1620.191 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.278 kb on - strand at 1620.278 kb on - strand at 1620.278 kb on - strand at 1620.278 kb on - strand at 1620.283 kb on - strand at 1620.296 kb on + strand at 1620.299 kb on + strand at 1620.299 kb on + strand at 1620.300 kb on - strand at 1620.300 kb on - strand at 1620.305 kb on - strand at 1620.314 kb on + strand at 1620.315 kb on - strand at 1620.329 kb on + strand at 1620.329 kb on + strand at 1620.330 kb on - strand at 1620.330 kb on - strand at 1620.381 kb on + strand at 1620.381 kb on + strand at 1620.381 kb on + strand at 1620.392 kb on - strand, within Psyr_1431 at 1620.412 kb on + strand, within Psyr_1431 at 1620.447 kb on + strand, within Psyr_1431 at 1620.447 kb on + strand, within Psyr_1431 at 1620.448 kb on - strand, within Psyr_1431 at 1620.448 kb on - strand, within Psyr_1431 at 1620.448 kb on - strand, within Psyr_1431 at 1620.498 kb on + strand, within Psyr_1431 at 1620.556 kb on + strand, within Psyr_1431 at 1620.556 kb on + strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.559 kb on + strand, within Psyr_1431 at 1620.559 kb on + strand, within Psyr_1431 at 1620.560 kb on - strand, within Psyr_1431
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,618,119 - -0.6 1,618,119 - +2.4 1,618,142 + +3.4 1,618,142 + -2.4 1,618,142 + +2.5 1,618,143 - -2.3 1,618,143 - +0.1 1,618,143 - -4.0 1,618,143 - -1.7 1,618,177 + +2.9 1,618,177 + -0.3 1,618,286 + Psyr_1429 0.13 -0.8 1,618,287 - Psyr_1429 0.13 -0.7 1,618,330 + Psyr_1429 0.18 +4.0 1,618,407 + Psyr_1429 0.26 -0.6 1,618,408 - Psyr_1429 0.26 -1.5 1,618,476 + Psyr_1429 0.33 -0.7 1,618,476 + Psyr_1429 0.33 -2.0 1,618,476 + Psyr_1429 0.33 -3.5 1,618,477 - Psyr_1429 0.33 -0.7 1,618,477 - Psyr_1429 0.33 -1.6 1,618,477 - Psyr_1429 0.33 +3.0 1,618,477 - Psyr_1429 0.33 +1.6 1,618,477 - Psyr_1429 0.33 +1.0 1,618,513 - Psyr_1429 0.37 +3.2 1,618,513 - Psyr_1429 0.37 -1.0 1,618,585 - Psyr_1429 0.45 +2.9 1,618,585 - Psyr_1429 0.45 -3.0 1,618,650 + Psyr_1429 0.52 -0.6 1,618,650 + Psyr_1429 0.52 +2.4 1,618,650 + Psyr_1429 0.52 -2.0 1,618,650 + Psyr_1429 0.52 -0.2 1,618,651 - Psyr_1429 0.52 +3.0 1,618,651 - Psyr_1429 0.52 -2.8 1,618,651 - Psyr_1429 0.52 -0.7 1,618,651 - Psyr_1429 0.52 -1.3 1,618,700 - Psyr_1429 0.57 +4.9 1,618,700 - Psyr_1429 0.57 +2.6 1,618,728 - Psyr_1429 0.60 +0.0 1,618,728 - Psyr_1429 0.60 +2.0 1,618,759 + Psyr_1429 0.63 +3.0 1,618,759 + Psyr_1429 0.63 -1.8 1,618,760 - Psyr_1429 0.64 -0.5 1,618,866 + Psyr_1429 0.75 -0.2 1,618,866 + Psyr_1429 0.75 -0.1 1,618,919 + Psyr_1429 0.81 +3.0 1,618,919 + Psyr_1429 0.81 +2.6 1,618,920 - Psyr_1429 0.81 -0.1 1,618,920 - Psyr_1429 0.81 +0.2 1,618,974 + Psyr_1429 0.86 -0.1 1,618,974 + Psyr_1429 0.86 -0.6 1,618,974 + Psyr_1429 0.86 +1.7 1,618,975 - Psyr_1429 0.86 -0.4 1,618,975 - Psyr_1429 0.86 +3.2 1,618,975 - Psyr_1429 0.86 -0.4 1,619,187 + Psyr_1430 0.19 +3.2 1,619,187 + Psyr_1430 0.19 -0.2 1,619,198 + Psyr_1430 0.21 +0.0 1,619,198 + Psyr_1430 0.21 +2.0 1,619,198 + Psyr_1430 0.21 +2.4 1,619,199 - Psyr_1430 0.21 +3.2 1,619,199 - Psyr_1430 0.21 +2.6 1,619,199 - Psyr_1430 0.21 -0.4 1,619,263 + Psyr_1430 0.35 -2.6 1,619,263 + Psyr_1430 0.35 +2.6 1,619,281 + Psyr_1430 0.39 +0.5 1,619,282 - Psyr_1430 0.39 -0.5 1,619,501 - Psyr_1430 0.85 -1.0 1,619,501 - Psyr_1430 0.85 -0.5 1,619,501 - Psyr_1430 0.85 -0.1 1,619,572 + +0.0 1,619,572 + -0.0 1,619,572 + -3.3 1,619,573 - -0.5 1,619,579 + +2.6 1,619,579 + -0.5 1,619,718 + +2.0 1,619,718 + +2.4 1,619,719 - -2.8 1,619,722 + -0.9 1,619,746 + -1.4 1,619,746 + +1.8 1,619,753 - +2.9 1,619,803 + +2.0 1,619,804 - -0.6 1,619,804 - +1.0 1,619,860 + +3.5 1,619,860 + +2.9 1,619,860 + -1.7 1,619,860 + -1.0 1,619,860 + +2.6 1,619,860 + +4.0 1,619,860 + +0.0 1,619,860 + +0.5 1,619,860 + -1.0 1,619,861 - +0.0 1,619,861 - -2.7 1,619,861 - -0.4 1,619,861 - +1.6 1,619,861 - +2.9 1,619,913 - -3.0 1,619,954 + +3.6 1,619,954 + -1.1 1,619,954 + -0.5 1,619,954 + -0.2 1,619,954 + +4.1 1,619,954 + +3.1 1,619,955 - -1.5 1,619,955 - -0.8 1,619,955 - +2.9 1,619,955 - -2.1 1,619,956 + +2.9 1,619,986 - -4.3 1,620,048 + -0.9 1,620,065 + -0.9 1,620,065 + -3.5 1,620,066 - -3.0 1,620,066 - +2.4 1,620,066 - +0.8 1,620,092 + +1.6 1,620,101 - +1.6 1,620,136 + +4.0 1,620,136 + +0.3 1,620,137 - +3.1 1,620,137 - +0.6 1,620,137 - +1.5 1,620,137 - -4.9 1,620,177 + -0.9 1,620,178 - -0.4 1,620,178 - -0.8 1,620,178 - -3.7 1,620,191 + +3.5 1,620,191 + -0.8 1,620,191 + +3.4 1,620,277 + +2.8 1,620,277 + +2.4 1,620,277 + +0.6 1,620,277 + -2.1 1,620,277 + +3.8 1,620,277 + +3.4 1,620,278 - +2.4 1,620,278 - -1.0 1,620,278 - +3.2 1,620,278 - -0.6 1,620,283 - +2.0 1,620,296 + +0.2 1,620,299 + +0.1 1,620,299 + +3.4 1,620,300 - +1.1 1,620,300 - -1.4 1,620,305 - +0.1 1,620,314 + -0.7 1,620,315 - +0.5 1,620,329 + +4.3 1,620,329 + -1.2 1,620,330 - -1.6 1,620,330 - +1.6 1,620,381 + +4.6 1,620,381 + -1.1 1,620,381 + +0.5 1,620,392 - Psyr_1431 0.13 +1.1 1,620,412 + Psyr_1431 0.20 +2.1 1,620,447 + Psyr_1431 0.32 -0.5 1,620,447 + Psyr_1431 0.32 +0.1 1,620,448 - Psyr_1431 0.33 +3.5 1,620,448 - Psyr_1431 0.33 +1.6 1,620,448 - Psyr_1431 0.33 +0.1 1,620,498 + Psyr_1431 0.50 +3.8 1,620,556 + Psyr_1431 0.70 +0.1 1,620,556 + Psyr_1431 0.70 -1.3 1,620,557 - Psyr_1431 0.70 +0.7 1,620,557 - Psyr_1431 0.70 +2.1 1,620,557 - Psyr_1431 0.70 +2.4 1,620,559 + Psyr_1431 0.71 +3.1 1,620,559 + Psyr_1431 0.71 +2.4 1,620,560 - Psyr_1431 0.71 -0.3
Or see this region's nucleotide sequence