Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1406 and Psyr_1407 are separated by 117 nucleotides Psyr_1407 and Psyr_1408 are separated by 130 nucleotides Psyr_1408 and Psyr_1409 are separated by 85 nucleotides Psyr_1409 and Psyr_1410 are separated by 0 nucleotides
Psyr_1406: Psyr_1406 - aspartate--tRNA(Asn) ligase, at 1,592,180 to 1,593,955
_1406
Psyr_1407: Psyr_1407 - Protein of unknown function DUF28, at 1,594,073 to 1,594,819
_1407
Psyr_1408: Psyr_1408 - Holliday junction endonuclease RuvC, at 1,594,950 to 1,595,474
_1408
Psyr_1409: Psyr_1409 - Holliday junction DNA helicase subunit RuvA, at 1,595,560 to 1,596,168
_1409
Psyr_1410: Psyr_1410 - Holliday junction DNA helicase RuvB, at 1,596,169 to 1,597,230
_1410
Position (kb)
1594
1595
1596 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1593.993 kb on - strand at 1594.087 kb on + strand at 1594.087 kb on + strand at 1594.087 kb on + strand at 1594.614 kb on - strand, within Psyr_1407 at 1594.614 kb on - strand, within Psyr_1407 at 1594.722 kb on + strand, within Psyr_1407 at 1594.723 kb on - strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.773 kb on - strand at 1594.773 kb on - strand at 1594.831 kb on + strand at 1594.832 kb on - strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.949 kb on + strand at 1594.950 kb on - strand at 1594.958 kb on + strand at 1594.958 kb on + strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.996 kb on - strand at 1595.031 kb on + strand, within Psyr_1408 at 1595.031 kb on + strand, within Psyr_1408 at 1595.032 kb on - strand, within Psyr_1408 at 1595.125 kb on - strand, within Psyr_1408 at 1595.253 kb on + strand, within Psyr_1408 at 1595.254 kb on - strand, within Psyr_1408 at 1595.406 kb on - strand, within Psyr_1408 at 1595.473 kb on - strand at 1595.473 kb on - strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.493 kb on + strand at 1595.494 kb on - strand at 1595.494 kb on - strand at 1595.494 kb on - strand at 1595.501 kb on - strand at 1595.573 kb on + strand at 1595.741 kb on - strand, within Psyr_1409 at 1595.856 kb on - strand, within Psyr_1409 at 1596.173 kb on + strand at 1596.191 kb on + strand at 1596.191 kb on + strand at 1596.192 kb on - strand at 1596.262 kb on + strand at 1596.263 kb on - strand at 1596.426 kb on + strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,593,993 - +0.4 1,594,087 + -2.0 1,594,087 + +2.9 1,594,087 + +3.6 1,594,614 - Psyr_1407 0.72 -2.3 1,594,614 - Psyr_1407 0.72 -2.0 1,594,722 + Psyr_1407 0.87 -0.6 1,594,723 - Psyr_1407 0.87 +1.6 1,594,738 + Psyr_1407 0.89 -0.5 1,594,738 + Psyr_1407 0.89 +1.1 1,594,738 + Psyr_1407 0.89 +0.6 1,594,738 + Psyr_1407 0.89 +1.2 1,594,739 - Psyr_1407 0.89 +1.1 1,594,739 - Psyr_1407 0.89 +1.6 1,594,739 - Psyr_1407 0.89 -0.9 1,594,772 + +0.1 1,594,772 + +1.6 1,594,772 + -1.4 1,594,772 + +1.1 1,594,773 - +0.1 1,594,773 - -2.6 1,594,831 + +0.4 1,594,832 - -0.5 1,594,914 + +0.4 1,594,914 + +4.5 1,594,914 + -2.7 1,594,949 + -1.4 1,594,950 - -1.7 1,594,958 + -0.1 1,594,958 + +3.1 1,594,959 - +1.6 1,594,959 - -1.5 1,594,959 - -1.9 1,594,959 - -1.9 1,594,996 - +2.4 1,595,031 + Psyr_1408 0.15 -3.9 1,595,031 + Psyr_1408 0.15 +0.1 1,595,032 - Psyr_1408 0.16 -3.5 1,595,125 - Psyr_1408 0.33 +3.2 1,595,253 + Psyr_1408 0.58 +1.6 1,595,254 - Psyr_1408 0.58 +2.1 1,595,406 - Psyr_1408 0.87 -2.3 1,595,473 - +2.1 1,595,473 - +1.6 1,595,481 + -0.0 1,595,481 + +3.5 1,595,481 + +3.2 1,595,481 + +2.6 1,595,481 + -0.8 1,595,481 + -1.7 1,595,481 + +2.1 1,595,481 + +3.8 1,595,481 + +0.2 1,595,481 + +1.3 1,595,481 + +3.8 1,595,481 + +4.5 1,595,482 - -1.9 1,595,482 - +1.6 1,595,482 - -1.4 1,595,482 - +2.6 1,595,482 - -4.0 1,595,482 - -1.0 1,595,482 - -0.9 1,595,482 - +0.1 1,595,482 - -2.0 1,595,483 + -1.7 1,595,483 + -0.9 1,595,483 + +4.7 1,595,483 + -1.8 1,595,483 + -1.2 1,595,483 + -0.7 1,595,483 + -0.1 1,595,483 + +1.1 1,595,483 + +1.1 1,595,483 + +1.6 1,595,483 + -1.6 1,595,483 + +3.4 1,595,483 + +1.6 1,595,483 + +0.3 1,595,483 + -1.4 1,595,483 + +1.6 1,595,483 + -0.7 1,595,483 + -0.7 1,595,483 + +0.1 1,595,483 + +2.9 1,595,483 + -0.1 1,595,483 + +2.6 1,595,483 + -1.7 1,595,483 + +4.8 1,595,483 + +3.1 1,595,483 + -0.5 1,595,483 + +1.6 1,595,483 + -0.7 1,595,484 - +1.6 1,595,484 - -1.3 1,595,484 - +3.5 1,595,484 - +0.5 1,595,484 - +1.6 1,595,484 - -3.0 1,595,484 - +1.6 1,595,484 - -1.8 1,595,484 - -0.2 1,595,484 - -1.5 1,595,484 - +1.6 1,595,484 - -3.1 1,595,484 - +0.2 1,595,484 - +2.9 1,595,484 - +0.1 1,595,484 - +2.9 1,595,493 + +3.4 1,595,494 - -1.7 1,595,494 - +1.6 1,595,494 - -1.2 1,595,501 - -0.6 1,595,573 + +2.4 1,595,741 - Psyr_1409 0.30 -0.7 1,595,856 - Psyr_1409 0.49 +0.1 1,596,173 + -2.7 1,596,191 + +2.9 1,596,191 + -3.3 1,596,192 - +1.6 1,596,262 + -3.0 1,596,263 - +0.1 1,596,426 + Psyr_1410 0.24 +3.5 1,596,427 - Psyr_1410 0.24 -2.4
Or see this region's nucleotide sequence