Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1314 and Psyr_1315 overlap by 4 nucleotides Psyr_1315 and Psyr_1316 are separated by 79 nucleotides Psyr_1316 and Psyr_1317 are separated by 59 nucleotides
Psyr_1314: Psyr_1314 - lipoprotein, putative, at 1,492,224 to 1,493,120
_1314
Psyr_1315: Psyr_1315 - lipoprotein, putative, at 1,493,117 to 1,493,437
_1315
Psyr_1316: Psyr_1316 - OmpA/MotB, at 1,493,517 to 1,494,299
_1316
Psyr_1317: Psyr_1317 - conserved hypothetical protein, at 1,494,359 to 1,494,757
_1317
Position (kb) 1493
1494 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 1492.125 kb on - strand at 1492.125 kb on - strand at 1492.125 kb on - strand at 1492.125 kb on - strand at 1492.125 kb on - strand at 1492.125 kb on - strand at 1492.287 kb on + strand at 1492.287 kb on + strand at 1492.335 kb on + strand, within Psyr_1314 at 1492.421 kb on + strand, within Psyr_1314 at 1492.569 kb on + strand, within Psyr_1314 at 1492.569 kb on + strand, within Psyr_1314 at 1492.677 kb on + strand, within Psyr_1314 at 1492.677 kb on + strand, within Psyr_1314 at 1492.677 kb on + strand, within Psyr_1314 at 1492.678 kb on - strand, within Psyr_1314 at 1492.747 kb on - strand, within Psyr_1314 at 1492.935 kb on + strand, within Psyr_1314 at 1492.935 kb on + strand, within Psyr_1314 at 1492.935 kb on + strand, within Psyr_1314 at 1492.936 kb on - strand, within Psyr_1314 at 1493.032 kb on - strand at 1493.199 kb on - strand, within Psyr_1315 at 1493.205 kb on - strand, within Psyr_1315 at 1493.282 kb on + strand, within Psyr_1315 at 1493.282 kb on + strand, within Psyr_1315 at 1493.283 kb on - strand, within Psyr_1315 at 1493.283 kb on - strand, within Psyr_1315 at 1493.283 kb on - strand, within Psyr_1315 at 1493.283 kb on - strand, within Psyr_1315 at 1493.283 kb on - strand, within Psyr_1315 at 1493.350 kb on + strand, within Psyr_1315 at 1493.447 kb on - strand at 1493.479 kb on + strand at 1493.479 kb on + strand at 1493.518 kb on + strand at 1493.518 kb on + strand at 1493.518 kb on + strand at 1493.519 kb on - strand at 1493.565 kb on - strand at 1493.565 kb on - strand at 1493.627 kb on - strand, within Psyr_1316 at 1493.667 kb on + strand, within Psyr_1316 at 1493.668 kb on - strand, within Psyr_1316 at 1493.668 kb on - strand, within Psyr_1316 at 1493.680 kb on - strand, within Psyr_1316 at 1493.690 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.731 kb on + strand, within Psyr_1316 at 1493.732 kb on - strand, within Psyr_1316 at 1493.732 kb on - strand, within Psyr_1316 at 1493.732 kb on - strand, within Psyr_1316 at 1493.732 kb on - strand, within Psyr_1316 at 1493.732 kb on - strand, within Psyr_1316 at 1493.758 kb on + strand, within Psyr_1316 at 1493.759 kb on - strand, within Psyr_1316 at 1493.858 kb on - strand, within Psyr_1316 at 1493.892 kb on - strand, within Psyr_1316 at 1494.061 kb on + strand, within Psyr_1316 at 1494.062 kb on - strand, within Psyr_1316 at 1494.293 kb on + strand at 1494.300 kb on - strand at 1494.302 kb on + strand at 1494.302 kb on + strand at 1494.315 kb on + strand at 1494.316 kb on - strand at 1494.349 kb on + strand at 1494.350 kb on - strand at 1494.350 kb on - strand at 1494.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,492,125 - +3.8 1,492,125 - +3.4 1,492,125 - +3.0 1,492,125 - +3.0 1,492,125 - -0.6 1,492,125 - -0.3 1,492,287 + -0.8 1,492,287 + -1.0 1,492,335 + Psyr_1314 0.12 -0.6 1,492,421 + Psyr_1314 0.22 -1.7 1,492,569 + Psyr_1314 0.38 +1.0 1,492,569 + Psyr_1314 0.38 +2.6 1,492,677 + Psyr_1314 0.51 +0.0 1,492,677 + Psyr_1314 0.51 +2.4 1,492,677 + Psyr_1314 0.51 -2.7 1,492,678 - Psyr_1314 0.51 +2.6 1,492,747 - Psyr_1314 0.58 +2.0 1,492,935 + Psyr_1314 0.79 -1.7 1,492,935 + Psyr_1314 0.79 +0.5 1,492,935 + Psyr_1314 0.79 +3.8 1,492,936 - Psyr_1314 0.79 +4.0 1,493,032 - +4.8 1,493,199 - Psyr_1315 0.26 -0.6 1,493,205 - Psyr_1315 0.27 -0.1 1,493,282 + Psyr_1315 0.51 -2.9 1,493,282 + Psyr_1315 0.51 -1.9 1,493,283 - Psyr_1315 0.52 -0.4 1,493,283 - Psyr_1315 0.52 -3.3 1,493,283 - Psyr_1315 0.52 +2.4 1,493,283 - Psyr_1315 0.52 +2.6 1,493,283 - Psyr_1315 0.52 -1.4 1,493,350 + Psyr_1315 0.73 -3.2 1,493,447 - -0.3 1,493,479 + -1.2 1,493,479 + -1.4 1,493,518 + -0.3 1,493,518 + -0.6 1,493,518 + +0.9 1,493,519 - -2.0 1,493,565 - +3.2 1,493,565 - -1.2 1,493,627 - Psyr_1316 0.14 +2.8 1,493,667 + Psyr_1316 0.19 -0.5 1,493,668 - Psyr_1316 0.19 +3.5 1,493,668 - Psyr_1316 0.19 +3.0 1,493,680 - Psyr_1316 0.21 +3.7 1,493,690 + Psyr_1316 0.22 +0.2 1,493,731 + Psyr_1316 0.27 -1.0 1,493,731 + Psyr_1316 0.27 +1.4 1,493,731 + Psyr_1316 0.27 -0.4 1,493,731 + Psyr_1316 0.27 -1.6 1,493,731 + Psyr_1316 0.27 +3.4 1,493,731 + Psyr_1316 0.27 +0.0 1,493,731 + Psyr_1316 0.27 -1.9 1,493,732 - Psyr_1316 0.27 -1.3 1,493,732 - Psyr_1316 0.27 -0.5 1,493,732 - Psyr_1316 0.27 +1.6 1,493,732 - Psyr_1316 0.27 -2.0 1,493,732 - Psyr_1316 0.27 +0.1 1,493,758 + Psyr_1316 0.31 -1.4 1,493,759 - Psyr_1316 0.31 +2.8 1,493,858 - Psyr_1316 0.44 -3.4 1,493,892 - Psyr_1316 0.48 -0.1 1,494,061 + Psyr_1316 0.69 -3.4 1,494,062 - Psyr_1316 0.70 -1.7 1,494,293 + +2.0 1,494,300 - +2.8 1,494,302 + -1.1 1,494,302 + +2.4 1,494,315 + +0.5 1,494,316 - +2.4 1,494,349 + +2.0 1,494,350 - -0.7 1,494,350 - +4.0 1,494,350 - +0.0
Or see this region's nucleotide sequence