Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1277 and Psyr_1278 are separated by 77 nucleotides Psyr_1278 and Psyr_1279 are separated by 90 nucleotides
Psyr_1277: Psyr_1277 - formate-dependent phosphoribosylglycinamide formyltransferase, at 1,442,343 to 1,443,524
_1277
Psyr_1278: Psyr_1278 - Citrate-proton symport, at 1,443,602 to 1,444,972
_1278
Psyr_1279: Psyr_1279 - CBS:Transporter-associated region, at 1,445,063 to 1,446,310
_1279
Position (kb)
1443
1444
1445 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1442.764 kb on - strand, within Psyr_1277 at 1443.030 kb on + strand, within Psyr_1277 at 1443.031 kb on - strand, within Psyr_1277 at 1443.514 kb on + strand at 1443.514 kb on + strand at 1443.515 kb on - strand at 1443.515 kb on - strand at 1443.515 kb on - strand at 1443.515 kb on - strand at 1443.515 kb on - strand at 1443.537 kb on - strand at 1443.537 kb on - strand at 1443.614 kb on - strand at 1443.675 kb on + strand at 1443.675 kb on + strand at 1443.675 kb on + strand at 1443.676 kb on - strand at 1443.765 kb on + strand, within Psyr_1278 at 1443.765 kb on + strand, within Psyr_1278 at 1443.766 kb on - strand, within Psyr_1278 at 1443.766 kb on - strand, within Psyr_1278 at 1443.766 kb on - strand, within Psyr_1278 at 1443.787 kb on - strand, within Psyr_1278 at 1443.922 kb on + strand, within Psyr_1278 at 1443.922 kb on + strand, within Psyr_1278 at 1443.923 kb on - strand, within Psyr_1278 at 1444.067 kb on - strand, within Psyr_1278 at 1444.115 kb on - strand, within Psyr_1278 at 1444.116 kb on + strand, within Psyr_1278 at 1444.116 kb on + strand, within Psyr_1278 at 1444.116 kb on + strand, within Psyr_1278 at 1444.116 kb on + strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.117 kb on - strand, within Psyr_1278 at 1444.129 kb on - strand, within Psyr_1278 at 1444.269 kb on + strand, within Psyr_1278 at 1444.269 kb on + strand, within Psyr_1278 at 1444.269 kb on + strand, within Psyr_1278 at 1444.269 kb on + strand, within Psyr_1278 at 1444.269 kb on + strand, within Psyr_1278 at 1444.270 kb on - strand, within Psyr_1278 at 1444.270 kb on - strand, within Psyr_1278 at 1444.270 kb on - strand, within Psyr_1278 at 1444.270 kb on - strand, within Psyr_1278 at 1444.571 kb on + strand, within Psyr_1278 at 1444.571 kb on + strand, within Psyr_1278 at 1444.572 kb on - strand, within Psyr_1278 at 1444.755 kb on - strand, within Psyr_1278 at 1444.788 kb on + strand, within Psyr_1278 at 1444.788 kb on + strand, within Psyr_1278 at 1444.788 kb on + strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.789 kb on - strand, within Psyr_1278 at 1444.794 kb on + strand, within Psyr_1278 at 1444.794 kb on + strand, within Psyr_1278 at 1444.795 kb on - strand, within Psyr_1278 at 1444.795 kb on - strand, within Psyr_1278 at 1444.795 kb on - strand, within Psyr_1278 at 1444.795 kb on - strand, within Psyr_1278 at 1444.974 kb on + strand at 1444.974 kb on + strand at 1444.974 kb on + strand at 1444.974 kb on + strand at 1444.975 kb on - strand at 1444.975 kb on - strand at 1444.975 kb on - strand at 1444.975 kb on - strand at 1445.046 kb on + strand at 1445.046 kb on + strand at 1445.046 kb on + strand at 1445.047 kb on - strand at 1445.047 kb on - strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.064 kb on + strand at 1445.065 kb on - strand at 1445.065 kb on - strand at 1445.065 kb on - strand at 1445.156 kb on + strand at 1445.381 kb on - strand, within Psyr_1279 at 1445.416 kb on - strand, within Psyr_1279 at 1445.502 kb on + strand, within Psyr_1279 at 1445.503 kb on - strand, within Psyr_1279 at 1445.503 kb on - strand, within Psyr_1279 at 1445.512 kb on - strand, within Psyr_1279 at 1445.579 kb on - strand, within Psyr_1279 at 1445.595 kb on - strand, within Psyr_1279 at 1445.595 kb on - strand, within Psyr_1279 at 1445.595 kb on - strand, within Psyr_1279 at 1445.606 kb on - strand, within Psyr_1279 at 1445.606 kb on - strand, within Psyr_1279 at 1445.616 kb on + strand, within Psyr_1279 at 1445.617 kb on - strand, within Psyr_1279 at 1445.781 kb on + strand, within Psyr_1279 at 1445.782 kb on - strand, within Psyr_1279 at 1445.904 kb on + strand, within Psyr_1279 at 1445.905 kb on - strand, within Psyr_1279 at 1445.905 kb on - strand, within Psyr_1279
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,442,764 - Psyr_1277 0.36 +3.3 1,443,030 + Psyr_1277 0.58 -2.5 1,443,031 - Psyr_1277 0.58 +0.4 1,443,514 + +0.7 1,443,514 + +1.0 1,443,515 - -1.8 1,443,515 - -0.8 1,443,515 - +2.6 1,443,515 - +4.5 1,443,515 - -3.1 1,443,537 - +0.3 1,443,537 - +0.0 1,443,614 - +3.0 1,443,675 + -1.3 1,443,675 + -0.9 1,443,675 + -0.5 1,443,676 - -1.0 1,443,765 + Psyr_1278 0.12 +1.6 1,443,765 + Psyr_1278 0.12 +0.5 1,443,766 - Psyr_1278 0.12 -1.2 1,443,766 - Psyr_1278 0.12 +1.9 1,443,766 - Psyr_1278 0.12 +3.5 1,443,787 - Psyr_1278 0.13 -0.7 1,443,922 + Psyr_1278 0.23 +1.0 1,443,922 + Psyr_1278 0.23 -0.2 1,443,923 - Psyr_1278 0.23 +3.5 1,444,067 - Psyr_1278 0.34 +0.5 1,444,115 - Psyr_1278 0.37 -2.7 1,444,116 + Psyr_1278 0.37 +3.7 1,444,116 + Psyr_1278 0.37 -1.3 1,444,116 + Psyr_1278 0.37 +0.3 1,444,116 + Psyr_1278 0.37 -0.2 1,444,117 - Psyr_1278 0.38 +2.8 1,444,117 - Psyr_1278 0.38 -1.2 1,444,117 - Psyr_1278 0.38 +2.8 1,444,117 - Psyr_1278 0.38 +2.8 1,444,117 - Psyr_1278 0.38 -0.4 1,444,117 - Psyr_1278 0.38 +3.0 1,444,117 - Psyr_1278 0.38 -1.6 1,444,117 - Psyr_1278 0.38 +2.6 1,444,117 - Psyr_1278 0.38 -0.9 1,444,129 - Psyr_1278 0.38 -1.1 1,444,269 + Psyr_1278 0.49 -2.6 1,444,269 + Psyr_1278 0.49 +2.7 1,444,269 + Psyr_1278 0.49 -2.1 1,444,269 + Psyr_1278 0.49 -1.1 1,444,269 + Psyr_1278 0.49 -1.0 1,444,270 - Psyr_1278 0.49 -0.8 1,444,270 - Psyr_1278 0.49 +4.1 1,444,270 - Psyr_1278 0.49 +2.3 1,444,270 - Psyr_1278 0.49 +0.2 1,444,571 + Psyr_1278 0.71 +4.8 1,444,571 + Psyr_1278 0.71 +0.5 1,444,572 - Psyr_1278 0.71 -0.4 1,444,755 - Psyr_1278 0.84 +0.0 1,444,788 + Psyr_1278 0.87 +0.9 1,444,788 + Psyr_1278 0.87 -1.3 1,444,788 + Psyr_1278 0.87 +3.2 1,444,789 - Psyr_1278 0.87 +4.2 1,444,789 - Psyr_1278 0.87 -0.8 1,444,789 - Psyr_1278 0.87 -1.1 1,444,789 - Psyr_1278 0.87 +3.7 1,444,789 - Psyr_1278 0.87 +0.8 1,444,789 - Psyr_1278 0.87 -1.3 1,444,789 - Psyr_1278 0.87 +3.2 1,444,789 - Psyr_1278 0.87 +4.4 1,444,794 + Psyr_1278 0.87 -2.4 1,444,794 + Psyr_1278 0.87 -3.0 1,444,795 - Psyr_1278 0.87 +1.6 1,444,795 - Psyr_1278 0.87 +1.6 1,444,795 - Psyr_1278 0.87 +3.8 1,444,795 - Psyr_1278 0.87 +0.1 1,444,974 + -0.4 1,444,974 + +1.3 1,444,974 + -2.5 1,444,974 + +2.6 1,444,975 - +1.6 1,444,975 - -0.4 1,444,975 - +1.6 1,444,975 - -1.1 1,445,046 + +0.4 1,445,046 + -1.1 1,445,046 + -0.3 1,445,047 - -0.1 1,445,047 - +2.4 1,445,064 + +0.8 1,445,064 + +3.9 1,445,064 + -1.9 1,445,064 + -2.2 1,445,064 + -3.0 1,445,064 + -1.8 1,445,064 + -1.1 1,445,065 - +3.0 1,445,065 - -1.8 1,445,065 - -0.3 1,445,156 + +2.6 1,445,381 - Psyr_1279 0.25 -0.5 1,445,416 - Psyr_1279 0.28 +1.3 1,445,502 + Psyr_1279 0.35 +0.4 1,445,503 - Psyr_1279 0.35 +2.6 1,445,503 - Psyr_1279 0.35 +0.5 1,445,512 - Psyr_1279 0.36 +3.3 1,445,579 - Psyr_1279 0.41 -0.6 1,445,595 - Psyr_1279 0.43 +2.3 1,445,595 - Psyr_1279 0.43 -0.1 1,445,595 - Psyr_1279 0.43 +2.8 1,445,606 - Psyr_1279 0.44 +3.5 1,445,606 - Psyr_1279 0.44 +0.7 1,445,616 + Psyr_1279 0.44 -1.2 1,445,617 - Psyr_1279 0.44 -1.2 1,445,781 + Psyr_1279 0.58 -1.4 1,445,782 - Psyr_1279 0.58 -1.0 1,445,904 + Psyr_1279 0.67 -0.9 1,445,905 - Psyr_1279 0.67 +3.5 1,445,905 - Psyr_1279 0.67 -1.2
Or see this region's nucleotide sequence