Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1266 and Psyr_1267 overlap by 1 nucleotides Psyr_1267 and Psyr_1268 overlap by 4 nucleotides Psyr_1268 and Psyr_1269 are separated by 213 nucleotides
Psyr_1266: Psyr_1266 - Twin-arginine translocation pathway signal, at 1,429,748 to 1,431,121
_1266
Psyr_1267: Psyr_1267 - tRNA-adenosine deaminase, at 1,431,121 to 1,431,630
_1267
Psyr_1268: Psyr_1268 - extracellular solute-binding protein, family 3:SLT, at 1,431,627 to 1,433,123
_1268
Psyr_1269: Psyr_1269 - phosphoribosylformylglycinamidine synthase, at 1,433,337 to 1,437,233
_1269
Position (kb)
1431
1432
1433
1434 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1430.704 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.876 kb on + strand, within Psyr_1266 at 1430.877 kb on - strand, within Psyr_1266 at 1430.877 kb on - strand, within Psyr_1266 at 1430.878 kb on + strand, within Psyr_1266 at 1430.878 kb on + strand, within Psyr_1266 at 1430.879 kb on - strand, within Psyr_1266 at 1430.879 kb on - strand, within Psyr_1266 at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.051 kb on + strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.052 kb on - strand at 1431.628 kb on + strand at 1431.905 kb on + strand, within Psyr_1268 at 1431.974 kb on - strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.035 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.039 kb on + strand, within Psyr_1268 at 1432.040 kb on - strand, within Psyr_1268 at 1432.048 kb on + strand, within Psyr_1268 at 1432.048 kb on + strand, within Psyr_1268 at 1432.258 kb on + strand, within Psyr_1268 at 1432.297 kb on + strand, within Psyr_1268 at 1432.297 kb on + strand, within Psyr_1268 at 1432.310 kb on - strand, within Psyr_1268 at 1432.310 kb on - strand, within Psyr_1268 at 1432.310 kb on - strand, within Psyr_1268 at 1432.334 kb on - strand, within Psyr_1268 at 1432.334 kb on - strand, within Psyr_1268 at 1432.337 kb on - strand, within Psyr_1268 at 1432.473 kb on + strand, within Psyr_1268 at 1432.479 kb on + strand, within Psyr_1268 at 1432.479 kb on + strand, within Psyr_1268 at 1432.479 kb on + strand, within Psyr_1268 at 1432.479 kb on + strand, within Psyr_1268 at 1432.480 kb on - strand, within Psyr_1268 at 1432.480 kb on - strand, within Psyr_1268 at 1432.492 kb on + strand, within Psyr_1268 at 1432.493 kb on - strand, within Psyr_1268 at 1432.493 kb on - strand, within Psyr_1268 at 1432.753 kb on + strand, within Psyr_1268 at 1432.754 kb on - strand, within Psyr_1268 at 1432.963 kb on + strand, within Psyr_1268 at 1433.132 kb on + strand at 1433.132 kb on + strand at 1433.133 kb on - strand at 1433.133 kb on - strand at 1433.134 kb on + strand at 1433.134 kb on + strand at 1433.134 kb on + strand at 1433.135 kb on - strand at 1433.135 kb on - strand at 1433.158 kb on - strand at 1433.184 kb on + strand at 1433.284 kb on - strand at 1433.284 kb on - strand at 1433.670 kb on + strand at 1433.670 kb on + strand at 1433.670 kb on + strand at 1433.670 kb on + strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.671 kb on - strand at 1433.674 kb on + strand at 1433.674 kb on + strand at 1433.674 kb on + strand at 1433.674 kb on + strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.675 kb on - strand at 1433.895 kb on + strand, within Psyr_1269 at 1434.064 kb on - strand, within Psyr_1269 at 1434.064 kb on - strand, within Psyr_1269 at 1434.096 kb on + strand, within Psyr_1269
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,430,704 + Psyr_1266 0.70 -3.4 1,430,876 + Psyr_1266 0.82 -2.6 1,430,876 + Psyr_1266 0.82 -2.8 1,430,876 + Psyr_1266 0.82 +3.4 1,430,876 + Psyr_1266 0.82 -1.1 1,430,876 + Psyr_1266 0.82 -3.8 1,430,876 + Psyr_1266 0.82 +3.3 1,430,876 + Psyr_1266 0.82 +2.0 1,430,876 + Psyr_1266 0.82 -1.9 1,430,876 + Psyr_1266 0.82 +3.0 1,430,877 - Psyr_1266 0.82 +2.0 1,430,877 - Psyr_1266 0.82 -0.5 1,430,878 + Psyr_1266 0.82 -2.0 1,430,878 + Psyr_1266 0.82 +2.8 1,430,879 - Psyr_1266 0.82 +3.4 1,430,879 - Psyr_1266 0.82 -2.7 1,431,051 + -1.4 1,431,051 + +1.6 1,431,051 + +1.4 1,431,051 + +3.7 1,431,051 + +1.0 1,431,051 + -1.8 1,431,051 + -0.5 1,431,052 - -0.4 1,431,052 - -1.2 1,431,052 - +2.8 1,431,052 - -0.1 1,431,052 - +2.8 1,431,052 - +2.0 1,431,628 + -2.4 1,431,905 + Psyr_1268 0.19 -0.0 1,431,974 - Psyr_1268 0.23 -0.0 1,432,035 + Psyr_1268 0.27 -3.8 1,432,035 + Psyr_1268 0.27 -0.0 1,432,035 + Psyr_1268 0.27 -2.8 1,432,039 + Psyr_1268 0.28 -4.0 1,432,039 + Psyr_1268 0.28 -5.7 1,432,039 + Psyr_1268 0.28 +1.6 1,432,039 + Psyr_1268 0.28 -0.0 1,432,040 - Psyr_1268 0.28 -0.0 1,432,048 + Psyr_1268 0.28 -3.2 1,432,048 + Psyr_1268 0.28 -2.3 1,432,258 + Psyr_1268 0.42 -0.0 1,432,297 + Psyr_1268 0.45 -3.3 1,432,297 + Psyr_1268 0.45 -1.8 1,432,310 - Psyr_1268 0.46 -0.0 1,432,310 - Psyr_1268 0.46 -4.4 1,432,310 - Psyr_1268 0.46 -3.3 1,432,334 - Psyr_1268 0.47 -4.0 1,432,334 - Psyr_1268 0.47 -3.5 1,432,337 - Psyr_1268 0.47 -4.5 1,432,473 + Psyr_1268 0.57 -0.0 1,432,479 + Psyr_1268 0.57 -0.0 1,432,479 + Psyr_1268 0.57 -3.9 1,432,479 + Psyr_1268 0.57 -2.8 1,432,479 + Psyr_1268 0.57 -0.6 1,432,480 - Psyr_1268 0.57 -2.6 1,432,480 - Psyr_1268 0.57 -0.0 1,432,492 + Psyr_1268 0.58 -0.0 1,432,493 - Psyr_1268 0.58 -1.6 1,432,493 - Psyr_1268 0.58 -4.1 1,432,753 + Psyr_1268 0.75 -0.0 1,432,754 - Psyr_1268 0.75 -4.0 1,432,963 + Psyr_1268 0.89 -2.8 1,433,132 + -4.0 1,433,132 + -2.0 1,433,133 - +1.7 1,433,133 - +0.1 1,433,134 + -0.7 1,433,134 + -1.8 1,433,134 + -0.5 1,433,135 - -0.8 1,433,135 - +2.8 1,433,158 - -0.4 1,433,184 + +3.8 1,433,284 - -0.0 1,433,284 - -0.0 1,433,670 + -0.0 1,433,670 + +0.7 1,433,670 + +2.3 1,433,670 + -2.8 1,433,671 - +2.6 1,433,671 - -2.0 1,433,671 - -3.2 1,433,671 - +1.0 1,433,671 - +2.4 1,433,671 - +3.0 1,433,671 - -1.1 1,433,671 - +2.0 1,433,671 - +1.0 1,433,674 + -2.0 1,433,674 + -3.6 1,433,674 + -2.3 1,433,674 + +1.6 1,433,675 - -2.0 1,433,675 - -0.0 1,433,675 - +1.6 1,433,675 - +2.3 1,433,675 - -1.7 1,433,675 - +2.8 1,433,675 - -4.1 1,433,895 + Psyr_1269 0.14 +1.0 1,434,064 - Psyr_1269 0.19 +2.6 1,434,064 - Psyr_1269 0.19 -1.0 1,434,096 + Psyr_1269 0.19 -0.3
Or see this region's nucleotide sequence