Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1226 and Psyr_RNA20 are separated by 202 nucleotides Psyr_RNA20 and Psyr_1227 are separated by 117 nucleotides Psyr_1227 and Psyr_1228 overlap by 4 nucleotides
Psyr_1226: Psyr_1226 - hypothetical protein, at 1,384,596 to 1,384,949
_1226
Psyr_RNA20: Psyr_RNA20 - tRNA-Leu, at 1,385,152 to 1,385,236
_RNA20
Psyr_1227: Psyr_1227 - Queuosine biosynthesis protein, at 1,385,354 to 1,386,418
_1227
Psyr_1228: Psyr_1228 - tRNA-guanine transglycosylase, at 1,386,415 to 1,387,548
_1228
Position (kb) 1385
1386
1387 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1384.394 kb on + strand at 1384.395 kb on - strand at 1384.399 kb on + strand at 1384.400 kb on - strand at 1384.429 kb on + strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.457 kb on + strand at 1384.482 kb on + strand at 1384.482 kb on + strand at 1384.601 kb on - strand at 1384.601 kb on - strand at 1384.601 kb on - strand at 1384.753 kb on - strand, within Psyr_1226 at 1384.777 kb on - strand, within Psyr_1226 at 1384.890 kb on + strand, within Psyr_1226 at 1384.897 kb on + strand, within Psyr_1226 at 1384.898 kb on - strand, within Psyr_1226 at 1385.032 kb on + strand at 1385.032 kb on + strand at 1385.032 kb on + strand at 1385.033 kb on - strand at 1385.033 kb on - strand at 1385.033 kb on - strand at 1385.041 kb on + strand at 1385.042 kb on - strand at 1385.042 kb on - strand at 1385.052 kb on + strand at 1385.052 kb on + strand at 1385.052 kb on + strand at 1385.053 kb on - strand at 1385.053 kb on - strand at 1385.053 kb on - strand at 1385.148 kb on + strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.287 kb on + strand at 1385.303 kb on + strand at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.797 kb on + strand, within Psyr_1227 at 1385.797 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1386.535 kb on + strand, within Psyr_1228 at 1387.416 kb on + strand, within Psyr_1228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,384,394 + +0.2 1,384,395 - -0.7 1,384,399 + +0.3 1,384,400 - -0.3 1,384,429 + -1.2 1,384,430 - -1.9 1,384,430 - +1.0 1,384,430 - -1.5 1,384,457 + +4.2 1,384,482 + +3.8 1,384,482 + -2.7 1,384,601 - -3.3 1,384,601 - +2.6 1,384,601 - +3.0 1,384,753 - Psyr_1226 0.44 +3.2 1,384,777 - Psyr_1226 0.51 +1.2 1,384,890 + Psyr_1226 0.83 -0.1 1,384,897 + Psyr_1226 0.85 -0.3 1,384,898 - Psyr_1226 0.85 -0.3 1,385,032 + -1.3 1,385,032 + +2.0 1,385,032 + +3.4 1,385,033 - +1.0 1,385,033 - -2.4 1,385,033 - +3.2 1,385,041 + -0.9 1,385,042 - +1.1 1,385,042 - +3.2 1,385,052 + +0.8 1,385,052 + +0.8 1,385,052 + -0.5 1,385,053 - -1.6 1,385,053 - +2.3 1,385,053 - +2.6 1,385,148 + +3.2 1,385,149 - -0.0 1,385,149 - +1.0 1,385,149 - -1.1 1,385,149 - -1.7 1,385,149 - -1.3 1,385,149 - -0.0 1,385,149 - +0.8 1,385,149 - -1.5 1,385,149 - +0.4 1,385,149 - -0.0 1,385,149 - -0.6 1,385,149 - +2.3 1,385,149 - -0.0 1,385,149 - -0.4 1,385,287 + -2.0 1,385,303 + -0.9 1,385,795 + Psyr_1227 0.41 -0.0 1,385,795 + Psyr_1227 0.41 -2.5 1,385,795 + Psyr_1227 0.41 -0.0 1,385,795 + Psyr_1227 0.41 -0.0 1,385,797 + Psyr_1227 0.42 -3.6 1,385,797 + Psyr_1227 0.42 -0.0 1,385,799 + Psyr_1227 0.42 -0.0 1,385,799 + Psyr_1227 0.42 -0.0 1,385,799 + Psyr_1227 0.42 +1.6 1,386,535 + Psyr_1228 0.11 +1.6 1,387,416 + Psyr_1228 0.88 +2.0
Or see this region's nucleotide sequence