Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1121 and Psyr_1122 are separated by 36 nucleotides Psyr_1122 and Psyr_1123 are separated by 127 nucleotides Psyr_1123 and Psyr_1124 are separated by 218 nucleotides Psyr_1124 and Psyr_1125 are separated by 70 nucleotides
Psyr_1121: Psyr_1121 - 6-phosphogluconolactonase, at 1,270,698 to 1,271,411
_1121
Psyr_1122: Psyr_1122 - 2-keto-3-deoxy-phosphogluconate aldolase, at 1,271,448 to 1,272,122
_1122
Psyr_1123: Psyr_1123 - conserved hypothetical protein, at 1,272,250 to 1,272,489
_1123
Psyr_1124: Psyr_1124 - conserved hypothetical protein, at 1,272,708 to 1,273,178
_1124
Psyr_1125: Psyr_1125 - GGDEF domain protein, at 1,273,249 to 1,274,778
_1125
Position (kb) 1272
1273 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1271.409 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.399 kb on - strand, within Psyr_1123 at 1272.410 kb on - strand, within Psyr_1123 at 1272.434 kb on + strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.522 kb on + strand at 1272.523 kb on - strand at 1272.523 kb on - strand at 1272.527 kb on + strand at 1272.658 kb on - strand at 1272.661 kb on + strand at 1272.662 kb on - strand at 1272.662 kb on - strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.845 kb on + strand, within Psyr_1124 at 1272.925 kb on + strand, within Psyr_1124 at 1272.925 kb on + strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1273.141 kb on + strand at 1273.141 kb on + strand at 1273.141 kb on + strand at 1273.142 kb on - strand at 1273.142 kb on - strand at 1273.142 kb on - strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.177 kb on - strand at 1273.231 kb on - strand at 1273.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,271,409 + -1.0 1,272,185 + +0.1 1,272,185 + -2.0 1,272,185 + +0.9 1,272,185 + +1.0 1,272,186 - +0.0 1,272,186 - -0.2 1,272,186 - -3.4 1,272,399 - Psyr_1123 0.62 +0.0 1,272,410 - Psyr_1123 0.67 -1.6 1,272,434 + Psyr_1123 0.77 +1.6 1,272,435 - Psyr_1123 0.77 -1.4 1,272,435 - Psyr_1123 0.77 -0.6 1,272,435 - Psyr_1123 0.77 -0.9 1,272,435 - Psyr_1123 0.77 -2.8 1,272,522 + +3.5 1,272,523 - +3.0 1,272,523 - +2.3 1,272,527 + +0.5 1,272,658 - -1.3 1,272,661 + -0.4 1,272,662 - -2.1 1,272,662 - -2.2 1,272,663 + +0.4 1,272,663 + -0.9 1,272,663 + +4.8 1,272,663 + +3.0 1,272,663 + +4.0 1,272,663 + -0.7 1,272,663 + +0.7 1,272,664 - -0.6 1,272,664 - -0.9 1,272,664 - +3.6 1,272,664 - -0.4 1,272,664 - +3.9 1,272,664 - +0.7 1,272,845 + Psyr_1124 0.29 +3.6 1,272,925 + Psyr_1124 0.46 -1.2 1,272,925 + Psyr_1124 0.46 +2.3 1,272,926 - Psyr_1124 0.46 +2.6 1,272,926 - Psyr_1124 0.46 +1.6 1,272,926 - Psyr_1124 0.46 +4.1 1,272,926 - Psyr_1124 0.46 -0.7 1,272,971 + Psyr_1124 0.56 -2.3 1,272,971 + Psyr_1124 0.56 +0.9 1,272,971 + Psyr_1124 0.56 -3.6 1,273,141 + +3.6 1,273,141 + +2.8 1,273,141 + -0.3 1,273,142 - +0.0 1,273,142 - -4.6 1,273,142 - -1.2 1,273,176 + +3.3 1,273,176 + +4.5 1,273,176 + -2.6 1,273,176 + +0.7 1,273,177 - -1.3 1,273,231 - +2.3 1,273,325 + -0.9
Or see this region's nucleotide sequence