Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1120 and Psyr_1121 overlap by 14 nucleotides Psyr_1121 and Psyr_1122 are separated by 36 nucleotides Psyr_1122 and Psyr_1123 are separated by 127 nucleotides
Psyr_1120: Psyr_1120 - glucose-6-phosphate 1-dehydrogenase, at 1,269,242 to 1,270,711
_1120
Psyr_1121: Psyr_1121 - 6-phosphogluconolactonase, at 1,270,698 to 1,271,411
_1121
Psyr_1122: Psyr_1122 - 2-keto-3-deoxy-phosphogluconate aldolase, at 1,271,448 to 1,272,122
_1122
Psyr_1123: Psyr_1123 - conserved hypothetical protein, at 1,272,250 to 1,272,489
_1123
Position (kb) 1270
1271
1272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1269.767 kb on + strand, within Psyr_1120 at 1269.767 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.850 kb on + strand, within Psyr_1120 at 1269.861 kb on + strand, within Psyr_1120 at 1269.861 kb on + strand, within Psyr_1120 at 1269.861 kb on + strand, within Psyr_1120 at 1269.861 kb on + strand, within Psyr_1120 at 1269.861 kb on + strand, within Psyr_1120 at 1269.908 kb on + strand, within Psyr_1120 at 1270.097 kb on + strand, within Psyr_1120 at 1270.235 kb on + strand, within Psyr_1120 at 1270.235 kb on + strand, within Psyr_1120 at 1270.235 kb on + strand, within Psyr_1120 at 1270.235 kb on + strand, within Psyr_1120 at 1270.235 kb on + strand, within Psyr_1120 at 1270.307 kb on + strand, within Psyr_1120 at 1270.307 kb on + strand, within Psyr_1120 at 1270.307 kb on + strand, within Psyr_1120 at 1270.307 kb on + strand, within Psyr_1120 at 1270.307 kb on + strand, within Psyr_1120 at 1270.469 kb on + strand, within Psyr_1120 at 1270.470 kb on - strand, within Psyr_1120 at 1270.496 kb on + strand, within Psyr_1120 at 1270.496 kb on + strand, within Psyr_1120 at 1270.496 kb on + strand, within Psyr_1120 at 1270.497 kb on - strand, within Psyr_1120 at 1270.565 kb on + strand at 1270.697 kb on + strand at 1271.031 kb on + strand, within Psyr_1121 at 1271.101 kb on + strand, within Psyr_1121 at 1271.101 kb on + strand, within Psyr_1121 at 1271.107 kb on + strand, within Psyr_1121 at 1271.409 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.399 kb on - strand, within Psyr_1123 at 1272.410 kb on - strand, within Psyr_1123
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,269,767 + Psyr_1120 0.36 +0.3 1,269,767 + Psyr_1120 0.36 -2.0 1,269,850 + Psyr_1120 0.41 -1.0 1,269,850 + Psyr_1120 0.41 -2.4 1,269,850 + Psyr_1120 0.41 -0.3 1,269,850 + Psyr_1120 0.41 +3.0 1,269,850 + Psyr_1120 0.41 +3.7 1,269,850 + Psyr_1120 0.41 +0.1 1,269,850 + Psyr_1120 0.41 -0.3 1,269,861 + Psyr_1120 0.42 +4.6 1,269,861 + Psyr_1120 0.42 -2.1 1,269,861 + Psyr_1120 0.42 +3.8 1,269,861 + Psyr_1120 0.42 -1.0 1,269,861 + Psyr_1120 0.42 +1.0 1,269,908 + Psyr_1120 0.45 -2.1 1,270,097 + Psyr_1120 0.58 -2.1 1,270,235 + Psyr_1120 0.68 +1.0 1,270,235 + Psyr_1120 0.68 -1.0 1,270,235 + Psyr_1120 0.68 -2.0 1,270,235 + Psyr_1120 0.68 +0.8 1,270,235 + Psyr_1120 0.68 +0.2 1,270,307 + Psyr_1120 0.72 -0.7 1,270,307 + Psyr_1120 0.72 +2.0 1,270,307 + Psyr_1120 0.72 +2.3 1,270,307 + Psyr_1120 0.72 +2.8 1,270,307 + Psyr_1120 0.72 +1.6 1,270,469 + Psyr_1120 0.83 -0.6 1,270,470 - Psyr_1120 0.84 +0.1 1,270,496 + Psyr_1120 0.85 +3.0 1,270,496 + Psyr_1120 0.85 -1.4 1,270,496 + Psyr_1120 0.85 -2.1 1,270,497 - Psyr_1120 0.85 +0.0 1,270,565 + +0.0 1,270,697 + +1.6 1,271,031 + Psyr_1121 0.47 +0.1 1,271,101 + Psyr_1121 0.56 +0.0 1,271,101 + Psyr_1121 0.56 -2.7 1,271,107 + Psyr_1121 0.57 -2.5 1,271,409 + -1.0 1,272,185 + +0.1 1,272,185 + -2.0 1,272,185 + +0.9 1,272,185 + +1.0 1,272,186 - +0.0 1,272,186 - -0.2 1,272,186 - -3.4 1,272,399 - Psyr_1123 0.62 +0.0 1,272,410 - Psyr_1123 0.67 -1.6
Or see this region's nucleotide sequence