Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1096 and Psyr_1097 are separated by 10 nucleotides Psyr_1097 and Psyr_1098 are separated by 325 nucleotides
Psyr_1096: Psyr_1096 - glycine dehydrogenase (decarboxylating) alpha subunit / glycine dehydrogenase (decarboxylating) beta subunit, at 1,238,600 to 1,241,464
_1096
Psyr_1097: Psyr_1097 - glycine cleavage system H protein, at 1,241,475 to 1,241,858
_1097
Psyr_1098: Psyr_1098 - response regulator receiver modulated diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s), at 1,242,184 to 1,244,301
_1098
Position (kb) 1241
1242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1240.495 kb on + strand, within Psyr_1096 at 1240.495 kb on + strand, within Psyr_1096 at 1240.496 kb on - strand, within Psyr_1096 at 1240.611 kb on + strand, within Psyr_1096 at 1240.611 kb on + strand, within Psyr_1096 at 1240.612 kb on - strand, within Psyr_1096 at 1240.612 kb on - strand, within Psyr_1096 at 1240.673 kb on + strand, within Psyr_1096 at 1240.673 kb on + strand, within Psyr_1096 at 1240.833 kb on + strand, within Psyr_1096 at 1240.834 kb on - strand, within Psyr_1096 at 1240.903 kb on + strand, within Psyr_1096 at 1241.133 kb on + strand, within Psyr_1096 at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.199 kb on + strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.200 kb on - strand at 1241.203 kb on - strand at 1241.203 kb on - strand at 1241.217 kb on + strand at 1241.217 kb on + strand at 1241.217 kb on + strand at 1241.217 kb on + strand at 1241.218 kb on - strand at 1241.218 kb on - strand at 1241.218 kb on - strand at 1241.719 kb on + strand, within Psyr_1097 at 1241.719 kb on + strand, within Psyr_1097 at 1241.720 kb on - strand, within Psyr_1097 at 1241.858 kb on + strand at 1241.858 kb on + strand at 1241.858 kb on + strand at 1241.859 kb on - strand at 1241.901 kb on + strand at 1241.901 kb on + strand at 1241.901 kb on + strand at 1241.902 kb on - strand at 1241.916 kb on - strand at 1241.916 kb on - strand at 1241.964 kb on + strand at 1242.024 kb on - strand at 1242.024 kb on - strand at 1242.024 kb on - strand at 1242.024 kb on - strand at 1242.033 kb on + strand at 1242.141 kb on - strand at 1242.151 kb on - strand at 1242.154 kb on - strand at 1242.257 kb on + strand at 1242.258 kb on - strand at 1242.444 kb on + strand, within Psyr_1098 at 1242.462 kb on - strand, within Psyr_1098 at 1242.504 kb on + strand, within Psyr_1098 at 1242.504 kb on + strand, within Psyr_1098 at 1242.805 kb on + strand, within Psyr_1098 at 1242.805 kb on + strand, within Psyr_1098 at 1242.806 kb on - strand, within Psyr_1098
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,240,495 + Psyr_1096 0.66 +2.8 1,240,495 + Psyr_1096 0.66 -1.9 1,240,496 - Psyr_1096 0.66 +3.7 1,240,611 + Psyr_1096 0.70 -2.6 1,240,611 + Psyr_1096 0.70 -0.9 1,240,612 - Psyr_1096 0.70 +2.6 1,240,612 - Psyr_1096 0.70 -0.9 1,240,673 + Psyr_1096 0.72 -0.3 1,240,673 + Psyr_1096 0.72 +2.0 1,240,833 + Psyr_1096 0.78 +4.5 1,240,834 - Psyr_1096 0.78 -0.3 1,240,903 + Psyr_1096 0.80 -1.0 1,241,133 + Psyr_1096 0.88 +2.3 1,241,199 + -1.1 1,241,199 + -1.4 1,241,199 + +3.6 1,241,199 + +0.0 1,241,199 + +2.6 1,241,199 + +2.8 1,241,199 + -0.6 1,241,199 + +0.2 1,241,199 + +2.2 1,241,199 + +0.4 1,241,200 - -1.7 1,241,200 - -2.7 1,241,200 - +2.6 1,241,200 - +3.6 1,241,200 - +1.0 1,241,200 - +2.0 1,241,200 - -2.8 1,241,200 - -0.6 1,241,200 - -1.3 1,241,200 - +3.6 1,241,200 - +0.0 1,241,200 - -0.3 1,241,200 - -1.7 1,241,203 - +3.5 1,241,203 - -1.3 1,241,217 + -1.6 1,241,217 + -0.6 1,241,217 + +1.6 1,241,217 + -0.2 1,241,218 - +1.0 1,241,218 - +2.3 1,241,218 - -2.3 1,241,719 + Psyr_1097 0.64 -3.7 1,241,719 + Psyr_1097 0.64 -1.8 1,241,720 - Psyr_1097 0.64 -3.7 1,241,858 + -1.5 1,241,858 + -1.5 1,241,858 + -0.4 1,241,859 - +1.0 1,241,901 + -2.2 1,241,901 + -0.3 1,241,901 + +3.2 1,241,902 - -1.2 1,241,916 - -2.6 1,241,916 - -2.2 1,241,964 + -1.7 1,242,024 - +1.0 1,242,024 - +2.8 1,242,024 - -1.9 1,242,024 - -1.8 1,242,033 + +3.5 1,242,141 - +1.9 1,242,151 - -1.6 1,242,154 - +2.0 1,242,257 + -0.5 1,242,258 - -0.5 1,242,444 + Psyr_1098 0.12 +0.7 1,242,462 - Psyr_1098 0.13 +0.0 1,242,504 + Psyr_1098 0.15 -1.4 1,242,504 + Psyr_1098 0.15 -2.0 1,242,805 + Psyr_1098 0.29 -1.9 1,242,805 + Psyr_1098 0.29 +2.3 1,242,806 - Psyr_1098 0.29 +3.0
Or see this region's nucleotide sequence