Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1048 and Psyr_1049 are separated by 71 nucleotides Psyr_1049 and Psyr_1050 are separated by 15 nucleotides Psyr_1050 and Psyr_1051 are separated by 211 nucleotides
Psyr_1048: Psyr_1048 - Secretion protein HlyD, at 1,184,228 to 1,185,088
_1048
Psyr_1049: Psyr_1049 - Short-chain dehydrogenase/reductase SDR, at 1,185,160 to 1,185,978
_1049
Psyr_1050: Psyr_1050 - conserved hypothetical protein, at 1,185,994 to 1,186,458
_1050
Psyr_1051: Psyr_1051 - YD repeat protein, at 1,186,670 to 1,189,453
_1051
Position (kb)
1185
1186
1187 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1185.089 kb on + strand at 1185.089 kb on + strand at 1185.090 kb on - strand at 1185.090 kb on - strand at 1185.090 kb on - strand at 1185.090 kb on - strand at 1185.090 kb on - strand at 1185.090 kb on - strand at 1185.144 kb on + strand at 1185.336 kb on + strand, within Psyr_1049 at 1185.337 kb on - strand, within Psyr_1049 at 1185.337 kb on - strand, within Psyr_1049 at 1185.337 kb on - strand, within Psyr_1049 at 1185.337 kb on - strand, within Psyr_1049 at 1185.487 kb on + strand, within Psyr_1049 at 1185.557 kb on + strand, within Psyr_1049 at 1185.673 kb on + strand, within Psyr_1049 at 1185.673 kb on + strand, within Psyr_1049 at 1185.674 kb on - strand, within Psyr_1049 at 1185.833 kb on - strand, within Psyr_1049 at 1185.961 kb on - strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.015 kb on + strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.016 kb on - strand at 1186.142 kb on + strand, within Psyr_1050 at 1186.142 kb on + strand, within Psyr_1050 at 1186.142 kb on + strand, within Psyr_1050 at 1186.143 kb on - strand, within Psyr_1050 at 1186.181 kb on + strand, within Psyr_1050 at 1186.182 kb on - strand, within Psyr_1050 at 1186.227 kb on + strand, within Psyr_1050 at 1186.280 kb on + strand, within Psyr_1050 at 1186.281 kb on - strand, within Psyr_1050 at 1186.281 kb on - strand, within Psyr_1050 at 1186.439 kb on - strand at 1186.493 kb on + strand at 1186.493 kb on + strand at 1186.494 kb on - strand at 1186.494 kb on - strand at 1186.494 kb on - strand at 1186.496 kb on + strand at 1186.497 kb on - strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.579 kb on + strand at 1186.580 kb on - strand at 1186.580 kb on - strand at 1186.580 kb on - strand at 1186.580 kb on - strand at 1186.580 kb on - strand at 1186.580 kb on - strand at 1186.608 kb on + strand at 1186.609 kb on - strand at 1186.609 kb on - strand at 1186.609 kb on - strand at 1186.609 kb on - strand at 1186.612 kb on + strand at 1186.613 kb on - strand at 1186.613 kb on - strand at 1186.654 kb on + strand at 1186.654 kb on + strand at 1186.655 kb on - strand at 1186.655 kb on - strand at 1186.655 kb on - strand at 1186.655 kb on - strand at 1186.655 kb on - strand at 1186.713 kb on + strand at 1186.714 kb on - strand at 1186.714 kb on - strand at 1186.714 kb on - strand at 1186.714 kb on - strand at 1186.974 kb on + strand, within Psyr_1051 at 1187.011 kb on + strand, within Psyr_1051 at 1187.011 kb on + strand, within Psyr_1051 at 1187.011 kb on + strand, within Psyr_1051 at 1187.012 kb on - strand, within Psyr_1051 at 1187.012 kb on - strand, within Psyr_1051 at 1187.012 kb on - strand, within Psyr_1051 at 1187.082 kb on - strand, within Psyr_1051 at 1187.105 kb on + strand, within Psyr_1051 at 1187.105 kb on + strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.106 kb on - strand, within Psyr_1051 at 1187.107 kb on + strand, within Psyr_1051 at 1187.107 kb on + strand, within Psyr_1051 at 1187.108 kb on - strand, within Psyr_1051 at 1187.108 kb on - strand, within Psyr_1051 at 1187.188 kb on + strand, within Psyr_1051 at 1187.188 kb on + strand, within Psyr_1051 at 1187.303 kb on + strand, within Psyr_1051 at 1187.304 kb on - strand, within Psyr_1051 at 1187.304 kb on - strand, within Psyr_1051 at 1187.304 kb on - strand, within Psyr_1051 at 1187.304 kb on - strand, within Psyr_1051 at 1187.304 kb on - strand, within Psyr_1051 at 1187.366 kb on + strand, within Psyr_1051 at 1187.367 kb on - strand, within Psyr_1051 at 1187.367 kb on - strand, within Psyr_1051 at 1187.367 kb on - strand, within Psyr_1051
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,185,089 + -1.8 1,185,089 + +2.6 1,185,090 - +2.8 1,185,090 - -1.6 1,185,090 - +0.0 1,185,090 - +3.8 1,185,090 - +2.8 1,185,090 - +0.0 1,185,144 + +0.3 1,185,336 + Psyr_1049 0.21 -1.1 1,185,337 - Psyr_1049 0.22 +4.3 1,185,337 - Psyr_1049 0.22 +3.5 1,185,337 - Psyr_1049 0.22 +1.3 1,185,337 - Psyr_1049 0.22 +3.5 1,185,487 + Psyr_1049 0.40 -0.8 1,185,557 + Psyr_1049 0.48 +0.0 1,185,673 + Psyr_1049 0.63 +4.9 1,185,673 + Psyr_1049 0.63 +3.7 1,185,674 - Psyr_1049 0.63 +0.8 1,185,833 - Psyr_1049 0.82 -4.1 1,185,961 - -0.8 1,186,015 + -2.9 1,186,015 + -1.9 1,186,015 + -1.4 1,186,015 + -1.9 1,186,015 + -0.1 1,186,015 + -3.8 1,186,015 + -1.1 1,186,015 + +0.2 1,186,016 - +1.3 1,186,016 - -0.1 1,186,016 - +2.8 1,186,016 - +3.4 1,186,016 - +3.2 1,186,016 - -1.1 1,186,016 - -0.4 1,186,016 - +0.6 1,186,016 - +1.6 1,186,142 + Psyr_1050 0.32 -1.0 1,186,142 + Psyr_1050 0.32 -2.1 1,186,142 + Psyr_1050 0.32 +1.0 1,186,143 - Psyr_1050 0.32 -1.6 1,186,181 + Psyr_1050 0.40 -2.2 1,186,182 - Psyr_1050 0.40 +3.6 1,186,227 + Psyr_1050 0.50 -0.7 1,186,280 + Psyr_1050 0.62 -2.0 1,186,281 - Psyr_1050 0.62 -1.4 1,186,281 - Psyr_1050 0.62 -1.1 1,186,439 - -0.9 1,186,493 + -1.5 1,186,493 + -0.6 1,186,494 - +2.8 1,186,494 - +1.6 1,186,494 - -1.4 1,186,496 + +0.1 1,186,497 - -1.8 1,186,579 + +5.0 1,186,579 + -0.2 1,186,579 + +2.6 1,186,579 + -1.3 1,186,579 + -0.4 1,186,579 + +1.0 1,186,579 + -1.6 1,186,579 + -0.8 1,186,579 + +0.1 1,186,580 - +2.6 1,186,580 - +2.4 1,186,580 - +0.0 1,186,580 - +3.6 1,186,580 - +1.8 1,186,580 - -0.5 1,186,608 + +0.3 1,186,609 - -0.9 1,186,609 - +1.8 1,186,609 - +0.0 1,186,609 - +0.9 1,186,612 + +3.0 1,186,613 - +2.4 1,186,613 - -1.1 1,186,654 + +2.8 1,186,654 + -0.8 1,186,655 - +2.6 1,186,655 - +4.1 1,186,655 - -0.9 1,186,655 - +4.0 1,186,655 - +2.4 1,186,713 + +2.0 1,186,714 - +2.4 1,186,714 - +1.3 1,186,714 - -2.0 1,186,714 - -2.4 1,186,974 + Psyr_1051 0.11 +4.2 1,187,011 + Psyr_1051 0.12 +2.0 1,187,011 + Psyr_1051 0.12 -0.2 1,187,011 + Psyr_1051 0.12 -1.9 1,187,012 - Psyr_1051 0.12 -1.2 1,187,012 - Psyr_1051 0.12 +1.0 1,187,012 - Psyr_1051 0.12 -0.6 1,187,082 - Psyr_1051 0.15 -1.0 1,187,105 + Psyr_1051 0.16 +1.0 1,187,105 + Psyr_1051 0.16 +0.0 1,187,106 - Psyr_1051 0.16 -1.1 1,187,106 - Psyr_1051 0.16 -1.8 1,187,106 - Psyr_1051 0.16 -0.2 1,187,106 - Psyr_1051 0.16 +3.5 1,187,106 - Psyr_1051 0.16 -0.5 1,187,106 - Psyr_1051 0.16 +0.4 1,187,107 + Psyr_1051 0.16 +2.8 1,187,107 + Psyr_1051 0.16 +0.0 1,187,108 - Psyr_1051 0.16 +3.5 1,187,108 - Psyr_1051 0.16 -1.1 1,187,188 + Psyr_1051 0.19 +1.0 1,187,188 + Psyr_1051 0.19 -1.4 1,187,303 + Psyr_1051 0.23 +3.5 1,187,304 - Psyr_1051 0.23 -0.7 1,187,304 - Psyr_1051 0.23 +1.0 1,187,304 - Psyr_1051 0.23 -1.3 1,187,304 - Psyr_1051 0.23 +3.5 1,187,304 - Psyr_1051 0.23 -0.9 1,187,366 + Psyr_1051 0.25 +4.2 1,187,367 - Psyr_1051 0.25 +2.0 1,187,367 - Psyr_1051 0.25 -1.1 1,187,367 - Psyr_1051 0.25 -1.0
Or see this region's nucleotide sequence