Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1009 and Psyr_1010 are separated by 131 nucleotides Psyr_1010 and Psyr_1011 are separated by 157 nucleotides Psyr_1011 and Psyr_1012 overlap by 32 nucleotides
Psyr_1009: Psyr_1009 - Peptidylprolyl isomerase, FKBP-type, at 1,143,819 to 1,144,304
_1009
Psyr_1010: Psyr_1010 - Glutathione peroxidase, at 1,144,436 to 1,144,924
_1010
Psyr_1011: Psyr_1011 - NADH:flavin oxidoreductase/NADH oxidase, at 1,145,082 to 1,146,185
_1011
Psyr_1012: Psyr_1012 - Glycosyl transferase, group 1, at 1,146,154 to 1,147,374
_1012
Position (kb)
1145
1146
1147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1144.315 kb on + strand at 1144.316 kb on - strand at 1144.402 kb on + strand at 1144.403 kb on - strand at 1144.403 kb on - strand at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.611 kb on + strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.705 kb on + strand, within Psyr_1010 at 1144.796 kb on - strand, within Psyr_1010 at 1144.796 kb on - strand, within Psyr_1010 at 1144.842 kb on + strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.867 kb on + strand, within Psyr_1010 at 1144.868 kb on - strand, within Psyr_1010 at 1144.939 kb on + strand at 1144.946 kb on + strand at 1144.946 kb on + strand at 1144.946 kb on + strand at 1144.947 kb on - strand at 1144.947 kb on - strand at 1144.947 kb on - strand at 1145.006 kb on + strand at 1145.007 kb on - strand at 1145.009 kb on + strand at 1145.010 kb on - strand at 1145.258 kb on + strand, within Psyr_1011 at 1145.259 kb on - strand, within Psyr_1011 at 1145.643 kb on - strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.773 kb on - strand, within Psyr_1011 at 1145.847 kb on - strand, within Psyr_1011 at 1145.948 kb on + strand, within Psyr_1011 at 1145.948 kb on + strand, within Psyr_1011 at 1145.949 kb on - strand, within Psyr_1011 at 1145.949 kb on - strand, within Psyr_1011 at 1146.429 kb on + strand, within Psyr_1012 at 1146.456 kb on - strand, within Psyr_1012 at 1146.538 kb on - strand, within Psyr_1012 at 1146.551 kb on + strand, within Psyr_1012 at 1146.551 kb on + strand, within Psyr_1012 at 1146.663 kb on + strand, within Psyr_1012 at 1146.664 kb on - strand, within Psyr_1012 at 1146.944 kb on + strand, within Psyr_1012 at 1146.945 kb on - strand, within Psyr_1012 at 1146.945 kb on - strand, within Psyr_1012 at 1146.980 kb on + strand, within Psyr_1012 at 1146.980 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,144,315 + +3.6 1,144,316 - -0.4 1,144,402 + -0.1 1,144,403 - -2.2 1,144,403 - -0.5 1,144,589 + Psyr_1010 0.31 -2.8 1,144,589 + Psyr_1010 0.31 +0.5 1,144,589 + Psyr_1010 0.31 -2.3 1,144,611 + Psyr_1010 0.36 +2.4 1,144,612 - Psyr_1010 0.36 -1.5 1,144,612 - Psyr_1010 0.36 -0.6 1,144,612 - Psyr_1010 0.36 -0.2 1,144,692 - Psyr_1010 0.52 -1.6 1,144,692 - Psyr_1010 0.52 +1.2 1,144,705 + Psyr_1010 0.55 -2.0 1,144,796 - Psyr_1010 0.74 -1.4 1,144,796 - Psyr_1010 0.74 -0.1 1,144,842 + Psyr_1010 0.83 -1.5 1,144,843 - Psyr_1010 0.83 -1.0 1,144,843 - Psyr_1010 0.83 -0.5 1,144,843 - Psyr_1010 0.83 +0.5 1,144,867 + Psyr_1010 0.88 +3.6 1,144,868 - Psyr_1010 0.88 +3.4 1,144,939 + +0.9 1,144,946 + +3.2 1,144,946 + -0.7 1,144,946 + -1.8 1,144,947 - -0.4 1,144,947 - +0.5 1,144,947 - +0.6 1,145,006 + +3.4 1,145,007 - -2.2 1,145,009 + +4.4 1,145,010 - -0.6 1,145,258 + Psyr_1011 0.16 +2.4 1,145,259 - Psyr_1011 0.16 +3.5 1,145,643 - Psyr_1011 0.51 +3.5 1,145,772 + Psyr_1011 0.62 +0.7 1,145,772 + Psyr_1011 0.62 -1.0 1,145,773 - Psyr_1011 0.63 +3.9 1,145,847 - Psyr_1011 0.69 -0.4 1,145,948 + Psyr_1011 0.78 -0.7 1,145,948 + Psyr_1011 0.78 -1.0 1,145,949 - Psyr_1011 0.79 +1.0 1,145,949 - Psyr_1011 0.79 +1.0 1,146,429 + Psyr_1012 0.23 -1.5 1,146,456 - Psyr_1012 0.25 +3.6 1,146,538 - Psyr_1012 0.31 +4.1 1,146,551 + Psyr_1012 0.33 -1.5 1,146,551 + Psyr_1012 0.33 +0.4 1,146,663 + Psyr_1012 0.42 +0.9 1,146,664 - Psyr_1012 0.42 +1.6 1,146,944 + Psyr_1012 0.65 +1.6 1,146,945 - Psyr_1012 0.65 +0.5 1,146,945 - Psyr_1012 0.65 -0.9 1,146,980 + Psyr_1012 0.68 +0.6 1,146,980 + Psyr_1012 0.68 -0.8 1,146,990 + Psyr_1012 0.68 +2.4 1,146,990 + Psyr_1012 0.68 +2.8 1,146,990 + Psyr_1012 0.68 -3.2 1,146,990 + Psyr_1012 0.68 -2.4 1,146,991 - Psyr_1012 0.69 -0.7 1,146,991 - Psyr_1012 0.69 -0.3 1,146,991 - Psyr_1012 0.69 -0.7 1,146,991 - Psyr_1012 0.69 -1.2 1,146,991 - Psyr_1012 0.69 +1.6 1,146,993 - Psyr_1012 0.69 +2.8 1,146,993 - Psyr_1012 0.69 -0.8 1,146,993 - Psyr_1012 0.69 +3.2 1,147,091 + Psyr_1012 0.77 +3.9 1,147,091 + Psyr_1012 0.77 +0.3 1,147,091 + Psyr_1012 0.77 -0.6 1,147,092 - Psyr_1012 0.77 +3.9 1,147,092 - Psyr_1012 0.77 -1.8 1,147,092 - Psyr_1012 0.77 -0.4 1,147,092 - Psyr_1012 0.77 +2.0 1,147,092 - Psyr_1012 0.77 -1.7 1,147,092 - Psyr_1012 0.77 +1.6 1,147,092 - Psyr_1012 0.77 -1.6 1,147,092 - Psyr_1012 0.77 -1.6
Or see this region's nucleotide sequence