Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1007 and Psyr_1008 are separated by 77 nucleotides Psyr_1008 and Psyr_1009 are separated by 92 nucleotides Psyr_1009 and Psyr_1010 are separated by 131 nucleotides
Psyr_1007: Psyr_1007 - phosphotransacetylase, at 1,141,331 to 1,143,421
_1007
Psyr_1008: Psyr_1008 - conserved hypothetical protein, at 1,143,499 to 1,143,726
_1008
Psyr_1009: Psyr_1009 - Peptidylprolyl isomerase, FKBP-type, at 1,143,819 to 1,144,304
_1009
Psyr_1010: Psyr_1010 - Glutathione peroxidase, at 1,144,436 to 1,144,924
_1010
Position (kb) 1143
1144 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 1142.579 kb on + strand, within Psyr_1007 at 1142.727 kb on + strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1143.075 kb on + strand, within Psyr_1007 at 1143.075 kb on + strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.323 kb on + strand at 1143.323 kb on + strand at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.525 kb on + strand, within Psyr_1008 at 1143.525 kb on + strand, within Psyr_1008 at 1143.667 kb on + strand, within Psyr_1008 at 1143.775 kb on + strand at 1143.799 kb on - strand at 1143.799 kb on - strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.858 kb on - strand at 1143.919 kb on - strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1144.029 kb on + strand, within Psyr_1009 at 1144.315 kb on + strand at 1144.316 kb on - strand at 1144.402 kb on + strand at 1144.403 kb on - strand at 1144.403 kb on - strand at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.611 kb on + strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.705 kb on + strand, within Psyr_1010
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,142,579 + Psyr_1007 0.60 +1.1 1,142,727 + Psyr_1007 0.67 +0.7 1,142,728 - Psyr_1007 0.67 -0.9 1,142,728 - Psyr_1007 0.67 -1.3 1,142,728 - Psyr_1007 0.67 -1.0 1,142,728 - Psyr_1007 0.67 -1.7 1,143,075 + Psyr_1007 0.83 -1.2 1,143,075 + Psyr_1007 0.83 +0.2 1,143,076 - Psyr_1007 0.83 -0.2 1,143,076 - Psyr_1007 0.83 +3.7 1,143,076 - Psyr_1007 0.83 -0.3 1,143,076 - Psyr_1007 0.83 +3.2 1,143,076 - Psyr_1007 0.83 -0.5 1,143,323 + +2.4 1,143,323 + +0.0 1,143,461 + +0.6 1,143,461 + +1.0 1,143,461 + +1.6 1,143,462 - +0.0 1,143,462 - -1.9 1,143,462 - +3.2 1,143,462 - -0.8 1,143,462 - -0.9 1,143,462 - -0.3 1,143,462 - +1.0 1,143,525 + Psyr_1008 0.11 -0.7 1,143,525 + Psyr_1008 0.11 +0.2 1,143,667 + Psyr_1008 0.74 +4.2 1,143,775 + +2.0 1,143,799 - -1.6 1,143,799 - -1.1 1,143,855 + +1.0 1,143,855 + +1.5 1,143,855 + -0.3 1,143,855 + +5.0 1,143,856 - +4.0 1,143,856 - +0.0 1,143,856 - -1.2 1,143,856 - -1.0 1,143,856 - -0.8 1,143,856 - +3.4 1,143,858 - -1.3 1,143,919 - Psyr_1009 0.21 -0.7 1,143,979 + Psyr_1009 0.33 -2.0 1,143,979 + Psyr_1009 0.33 +3.4 1,144,029 + Psyr_1009 0.43 +4.8 1,144,315 + +3.6 1,144,316 - -0.4 1,144,402 + -0.1 1,144,403 - -2.2 1,144,403 - -0.5 1,144,589 + Psyr_1010 0.31 -2.8 1,144,589 + Psyr_1010 0.31 +0.5 1,144,589 + Psyr_1010 0.31 -2.3 1,144,611 + Psyr_1010 0.36 +2.4 1,144,612 - Psyr_1010 0.36 -1.5 1,144,612 - Psyr_1010 0.36 -0.6 1,144,612 - Psyr_1010 0.36 -0.2 1,144,692 - Psyr_1010 0.52 -1.6 1,144,692 - Psyr_1010 0.52 +1.2 1,144,705 + Psyr_1010 0.55 -2.0
Or see this region's nucleotide sequence