Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1006 and Psyr_1007 are separated by 63 nucleotides Psyr_1007 and Psyr_1008 are separated by 77 nucleotides Psyr_1008 and Psyr_1009 are separated by 92 nucleotides
Psyr_1006: Psyr_1006 - Phospholipid/glycerol acyltransferase, at 1,140,362 to 1,141,267
_1006
Psyr_1007: Psyr_1007 - phosphotransacetylase, at 1,141,331 to 1,143,421
_1007
Psyr_1008: Psyr_1008 - conserved hypothetical protein, at 1,143,499 to 1,143,726
_1008
Psyr_1009: Psyr_1009 - Peptidylprolyl isomerase, FKBP-type, at 1,143,819 to 1,144,304
_1009
Position (kb)
1141
1142
1143
1144 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1140.828 kb on + strand, within Psyr_1006 at 1140.829 kb on - strand, within Psyr_1006 at 1140.829 kb on - strand, within Psyr_1006 at 1140.829 kb on - strand, within Psyr_1006 at 1140.937 kb on + strand, within Psyr_1006 at 1140.937 kb on + strand, within Psyr_1006 at 1140.937 kb on + strand, within Psyr_1006 at 1140.938 kb on - strand, within Psyr_1006 at 1140.938 kb on - strand, within Psyr_1006 at 1140.954 kb on + strand, within Psyr_1006 at 1140.954 kb on + strand, within Psyr_1006 at 1140.954 kb on + strand, within Psyr_1006 at 1140.955 kb on - strand, within Psyr_1006 at 1140.955 kb on - strand, within Psyr_1006 at 1140.955 kb on - strand, within Psyr_1006 at 1141.005 kb on + strand, within Psyr_1006 at 1141.005 kb on + strand, within Psyr_1006 at 1141.005 kb on + strand, within Psyr_1006 at 1141.006 kb on - strand, within Psyr_1006 at 1141.330 kb on - strand at 1141.330 kb on - strand at 1141.352 kb on + strand at 1141.353 kb on - strand at 1141.375 kb on - strand at 1141.375 kb on - strand at 1141.375 kb on - strand at 1141.375 kb on - strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.485 kb on + strand at 1141.486 kb on - strand at 1141.486 kb on - strand at 1141.486 kb on - strand at 1141.486 kb on - strand at 1141.486 kb on - strand at 1141.486 kb on - strand at 1141.493 kb on - strand at 1141.596 kb on + strand, within Psyr_1007 at 1141.597 kb on - strand, within Psyr_1007 at 1141.597 kb on - strand, within Psyr_1007 at 1141.619 kb on + strand, within Psyr_1007 at 1141.620 kb on - strand, within Psyr_1007 at 1141.620 kb on - strand, within Psyr_1007 at 1141.620 kb on - strand, within Psyr_1007 at 1141.620 kb on - strand, within Psyr_1007 at 1141.704 kb on + strand, within Psyr_1007 at 1141.705 kb on - strand, within Psyr_1007 at 1141.821 kb on + strand, within Psyr_1007 at 1141.822 kb on - strand, within Psyr_1007 at 1141.822 kb on - strand, within Psyr_1007 at 1141.822 kb on - strand, within Psyr_1007 at 1142.074 kb on - strand, within Psyr_1007 at 1142.074 kb on - strand, within Psyr_1007 at 1142.074 kb on - strand, within Psyr_1007 at 1142.463 kb on + strand, within Psyr_1007 at 1142.463 kb on + strand, within Psyr_1007 at 1142.463 kb on + strand, within Psyr_1007 at 1142.464 kb on - strand, within Psyr_1007 at 1142.579 kb on + strand, within Psyr_1007 at 1142.727 kb on + strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1142.728 kb on - strand, within Psyr_1007 at 1143.075 kb on + strand, within Psyr_1007 at 1143.075 kb on + strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.076 kb on - strand, within Psyr_1007 at 1143.323 kb on + strand at 1143.323 kb on + strand at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.525 kb on + strand, within Psyr_1008 at 1143.525 kb on + strand, within Psyr_1008 at 1143.667 kb on + strand, within Psyr_1008 at 1143.775 kb on + strand at 1143.799 kb on - strand at 1143.799 kb on - strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.858 kb on - strand at 1143.919 kb on - strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1144.029 kb on + strand, within Psyr_1009 at 1144.315 kb on + strand at 1144.316 kb on - strand at 1144.402 kb on + strand at 1144.403 kb on - strand at 1144.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,140,828 + Psyr_1006 0.51 +3.9 1,140,829 - Psyr_1006 0.52 +3.0 1,140,829 - Psyr_1006 0.52 -1.4 1,140,829 - Psyr_1006 0.52 -0.5 1,140,937 + Psyr_1006 0.63 +4.4 1,140,937 + Psyr_1006 0.63 +1.0 1,140,937 + Psyr_1006 0.63 -1.0 1,140,938 - Psyr_1006 0.64 +3.0 1,140,938 - Psyr_1006 0.64 +0.6 1,140,954 + Psyr_1006 0.65 -1.1 1,140,954 + Psyr_1006 0.65 -3.4 1,140,954 + Psyr_1006 0.65 +3.9 1,140,955 - Psyr_1006 0.65 +4.8 1,140,955 - Psyr_1006 0.65 +1.6 1,140,955 - Psyr_1006 0.65 -0.6 1,141,005 + Psyr_1006 0.71 +1.5 1,141,005 + Psyr_1006 0.71 +3.0 1,141,005 + Psyr_1006 0.71 -1.9 1,141,006 - Psyr_1006 0.71 -1.9 1,141,330 - +2.6 1,141,330 - +2.6 1,141,352 + +0.4 1,141,353 - -1.0 1,141,375 - +2.8 1,141,375 - +1.3 1,141,375 - -2.3 1,141,375 - +1.0 1,141,485 + +2.6 1,141,485 + +4.0 1,141,485 + +2.6 1,141,485 + -0.6 1,141,485 + -0.9 1,141,485 + +2.4 1,141,485 + -3.0 1,141,485 + +0.3 1,141,485 + +1.4 1,141,485 + -0.2 1,141,485 + -1.1 1,141,485 + +3.7 1,141,485 + -2.3 1,141,486 - -1.4 1,141,486 - -2.5 1,141,486 - +1.0 1,141,486 - -1.9 1,141,486 - -1.3 1,141,486 - -0.6 1,141,493 - +1.6 1,141,596 + Psyr_1007 0.13 -2.4 1,141,597 - Psyr_1007 0.13 -2.0 1,141,597 - Psyr_1007 0.13 -1.8 1,141,619 + Psyr_1007 0.14 -2.2 1,141,620 - Psyr_1007 0.14 -3.9 1,141,620 - Psyr_1007 0.14 +1.6 1,141,620 - Psyr_1007 0.14 +2.4 1,141,620 - Psyr_1007 0.14 -1.3 1,141,704 + Psyr_1007 0.18 +2.0 1,141,705 - Psyr_1007 0.18 +2.0 1,141,821 + Psyr_1007 0.23 +2.8 1,141,822 - Psyr_1007 0.23 +3.5 1,141,822 - Psyr_1007 0.23 +0.0 1,141,822 - Psyr_1007 0.23 +2.6 1,142,074 - Psyr_1007 0.36 -1.7 1,142,074 - Psyr_1007 0.36 +2.8 1,142,074 - Psyr_1007 0.36 +2.0 1,142,463 + Psyr_1007 0.54 +4.9 1,142,463 + Psyr_1007 0.54 -2.1 1,142,463 + Psyr_1007 0.54 +0.3 1,142,464 - Psyr_1007 0.54 +3.2 1,142,579 + Psyr_1007 0.60 +1.1 1,142,727 + Psyr_1007 0.67 +0.7 1,142,728 - Psyr_1007 0.67 -0.9 1,142,728 - Psyr_1007 0.67 -1.3 1,142,728 - Psyr_1007 0.67 -1.0 1,142,728 - Psyr_1007 0.67 -1.7 1,143,075 + Psyr_1007 0.83 -1.2 1,143,075 + Psyr_1007 0.83 +0.2 1,143,076 - Psyr_1007 0.83 -0.2 1,143,076 - Psyr_1007 0.83 +3.7 1,143,076 - Psyr_1007 0.83 -0.3 1,143,076 - Psyr_1007 0.83 +3.2 1,143,076 - Psyr_1007 0.83 -0.5 1,143,323 + +2.4 1,143,323 + +0.0 1,143,461 + +0.6 1,143,461 + +1.0 1,143,461 + +1.6 1,143,462 - +0.0 1,143,462 - -1.9 1,143,462 - +3.2 1,143,462 - -0.8 1,143,462 - -0.9 1,143,462 - -0.3 1,143,462 - +1.0 1,143,525 + Psyr_1008 0.11 -0.7 1,143,525 + Psyr_1008 0.11 +0.2 1,143,667 + Psyr_1008 0.74 +4.2 1,143,775 + +2.0 1,143,799 - -1.6 1,143,799 - -1.1 1,143,855 + +1.0 1,143,855 + +1.5 1,143,855 + -0.3 1,143,855 + +5.0 1,143,856 - +4.0 1,143,856 - +0.0 1,143,856 - -1.2 1,143,856 - -1.0 1,143,856 - -0.8 1,143,856 - +3.4 1,143,858 - -1.3 1,143,919 - Psyr_1009 0.21 -0.7 1,143,979 + Psyr_1009 0.33 -2.0 1,143,979 + Psyr_1009 0.33 +3.4 1,144,029 + Psyr_1009 0.43 +4.8 1,144,315 + +3.6 1,144,316 - -0.4 1,144,402 + -0.1 1,144,403 - -2.2 1,144,403 - -0.5
Or see this region's nucleotide sequence